infections. Enterococci may be the cause of diarrhea in children. Multidrug-resistant (MDR) Enterococci limits treatment options. So, the aim of our study was to detect the frequency and the antibiotic resistance profile, both phenotypically and genotypically, of MDR Enterococci isolated from the stools of children suffering from gastroenteritis who needed hospitalization. Materials and Methods: Enterococci were isolated from stool samples from children. One hundred ten (110) infants (in patients) with typical signs of gastroenteritis (nausea, vomiting, abdominal pain, diarrhea). The Vitek 2 Compact System is used for identification and antimicrobial susceptibility testing. Drug resistance genes (ermB, aph(3')IIIa, and tetM) and virulence genes (esp) were identified using molecular techniques. Results: Thirty-six isolates of Enterococci