Due to the development of praziquantel (PZQ) schistosomes resistant strains, the discovery of new antischistosomal agents is of high priority in research. This work reported the in vitro and in vivo effects of the edible and ornamental pomegranate extracts against Schistosoma mansoni. Leaves and stem bark ethanolic extracts of both dried pomegranates were prepared at 100, 300, and 500

Due to the development of praziquantel (PZQ) schistosomes resistant strains, the discovery of new antischistosomal agents is of high priority in research. This work reported the in vitro and in vivo effects of the edible and ornamental pomegranate extracts against Schistosoma mansoni. Leaves and stem bark ethanolic extracts of both dried pomegranates were prepared at 100, 300, and 500

Due to the development of praziquantel (PZQ) schistosomes resistant strains, the discovery of new antischistosomal agents is of high priority in research. This work reported the in vitro and in vivo effects of the edible and ornamental pomegranate extracts against Schistosoma mansoni. Leaves and stem bark ethanolic extracts of both dried pomegranates were prepared at 100, 300, and 500

Giardia and Cryptosporidium are wide spread pathogens of human and many species of mammals. This study aimed to investigate the potential direct transmission of Giardia and Cryptosporidium between cattle calves and the surrounding living children and to improve the knowledge of zoonotic Giardiasis and cryptosporidiosis situation in Assiut Governorate, Egypt. Faecal samples from 70 diarrheic children (above 5 years) and 62 diarrheic calves (1-3 months) were collected from the same villages at Assiut Governorate, Egypt. Samples were subjected to conventional microscopic examination for Giardia and Cryptosporidium. Positive samples were subjected to molecular identification. By conventional microscopic examination, Giardia cysts were detected in 27 out of 70 (38.57%) child’s stool samples, while Cryptosporidium oocyst were detected in 12 samples (17.14%). Mixed infection of Giardia and Cryptosporidium were detected in all Cryptosporidium positive samples. In children, 81.48% of Giardia samples were G. intestinalis assemblage B meanwhile, all samples were negative by PCR for C. parvum. In calves 29.03% of faecal samples were positive for Giardia by microscopic examination, while only 12.90% of those samples were positive for Cryptosporidium oocysts. 21 out of 26 (80.76%) Giardia positive samples were positive to G. intestinalis assemblage B and 22 out of 26 Cryptosporidium positive samples (84.61%) were positive for C. parvum. High prevalence of G. intestinalis assemblage B infection detected among children was significantly associated with contact with calves explaining the existence of its zoonotic transmission and more studies are needed to investigate the zoonotic potential of Cryptosporidium in the study area.

Giardia and Cryptosporidium are wide spread pathogens of human and many species of mammals. This study aimed to investigate the potential direct transmission of Giardia and Cryptosporidium between cattle calves and the surrounding living children and to improve the knowledge of zoonotic Giardiasis and cryptosporidiosis situation in Assiut Governorate, Egypt. Faecal samples from 70 diarrheic children (above 5 years) and 62 diarrheic calves (1-3 months) were collected from the same villages at Assiut Governorate, Egypt. Samples were subjected to conventional microscopic examination for Giardia and Cryptosporidium. Positive samples were subjected to molecular identification. By conventional microscopic examination, Giardia cysts were detected in 27 out of 70 (38.57%) child’s stool samples, while Cryptosporidium oocyst were detected in 12 samples (17.14%). Mixed infection of Giardia and Cryptosporidium were detected in all Cryptosporidium positive samples. In children, 81.48% of Giardia samples were G. intestinalis assemblage B meanwhile, all samples were negative by PCR for C. parvum. In calves 29.03% of faecal samples were positive for Giardia by microscopic examination, while only 12.90% of those samples were positive for Cryptosporidium oocysts. 21 out of 26 (80.76%) Giardia positive samples were positive to G. intestinalis assemblage B and 22 out of 26 Cryptosporidium positive samples (84.61%) were positive for C. parvum. High prevalence of G. intestinalis assemblage B infection detected among children was significantly associated with contact with calves explaining the existence of its zoonotic transmission and more studies are needed to investigate the zoonotic potential of Cryptosporidium in the study area.

Giardia and Cryptosporidium are wide spread pathogens of human and many species of mammals. This study aimed to investigate the potential direct transmission of Giardia and Cryptosporidium between cattle calves and the surrounding living children and to improve the knowledge of zoonotic Giardiasis and cryptosporidiosis situation in Assiut Governorate, Egypt. Faecal samples from 70 diarrheic children (above 5 years) and 62 diarrheic calves (1-3 months) were collected from the same villages at Assiut Governorate, Egypt. Samples were subjected to conventional microscopic examination for Giardia and Cryptosporidium. Positive samples were subjected to molecular identification. By conventional microscopic examination, Giardia cysts were detected in 27 out of 70 (38.57%) child’s stool samples, while Cryptosporidium oocyst were detected in 12 samples (17.14%). Mixed infection of Giardia and Cryptosporidium were detected in all Cryptosporidium positive samples. In children, 81.48% of Giardia samples were G. intestinalis assemblage B meanwhile, all samples were negative by PCR for C. parvum. In calves 29.03% of faecal samples were positive for Giardia by microscopic examination, while only 12.90% of those samples were positive for Cryptosporidium oocysts. 21 out of 26 (80.76%) Giardia positive samples were positive to G. intestinalis assemblage B and 22 out of 26 Cryptosporidium positive samples (84.61%) were positive for C. parvum. High prevalence of G. intestinalis assemblage B infection detected among children was significantly associated with contact with calves explaining the existence of its zoonotic transmission and more studies are needed to investigate the zoonotic potential of Cryptosporidium in the study area.

We are hereby reporting a case where the eggs and adults of the mold mites; Tyrophagus putrescentiae (Shrank) and the trophozoites of Blastocystis sp. were found in stool of three years old child from Minia City, Egypt. Intestinal mite infection was diagnosed after repeated identification of mite’ stages from six consecutive stool samples to exclude the possibilities of contamination and spurious infection. The patient was suffering from severe colicky abdominal pain and burning sensation around the anus one month ago. All other members of his family were having the same acarine in their feces, but were all symptomless. The patient was treated with ivermectin 200 μg/kg body weight once every 10 days for three doses. His cure indicated that he was having asymptomatic blastocystosis.

We are hereby reporting a case where the eggs and adults of the mold mites; Tyrophagus putrescentiae (Shrank) and the trophozoites of Blastocystis sp. were found in stool of three years old child from Minia City, Egypt. Intestinal mite infection was diagnosed after repeated identification of mite’ stages from six consecutive stool samples to exclude the possibilities of contamination and spurious infection. The patient was suffering from severe colicky abdominal pain and burning sensation around the anus one month ago. All other members of his family were having the same acarine in their feces, but were all symptomless. The patient was treated with ivermectin 200 μg/kg body weight once every 10 days for three doses. His cure indicated that he was having asymptomatic blastocystosis.

We are hereby reporting a case where the eggs and adults of the mold mites; Tyrophagus putrescentiae (Shrank) and the trophozoites of Blastocystis sp. were found in stool of three years old child from Minia City, Egypt. Intestinal mite infection was diagnosed after repeated identification of mite’ stages from six consecutive stool samples to exclude the possibilities of contamination and spurious infection. The patient was suffering from severe colicky abdominal pain and burning sensation around the anus one month ago. All other members of his family were having the same acarine in their feces, but were all symptomless. The patient was treated with ivermectin 200 μg/kg body weight once every 10 days for three doses. His cure indicated that he was having asymptomatic blastocystosis.

Objective Validated methods for the screening and early diagnosis of systemic sclerosis (SSc; scleroderma)–related interstitial lung disease (ILD) are needed. The aim of this study was to evaluate the performance of pulmonary function tests (PFTs) compared with that of high-resolution computed tomography (HRCT) of the chest for the detection of SSc-related ILD in clinical practice, and to identify predictors of lung involvement that is functionally occult but significant on HRCT. Methods Prospectively enrolled patients with SSc were assessed according to the European League Against Rheumatism (EULAR)/EULAR Scleroderma Trial and Research standards. The assessment included PFTs and HRCT. The HRCT images were evaluated in a blinded manner by 2 experienced radiologists. The performance parameters of PFTs for the diagnosis of SSc-related ILD were calculated. Predictors of significant ILD as determined by HRCT in patients with normal forced vital capacity (FVC) values were identified through logistic regression. Results Among the 102 patients, 64 (63.0%) showed significant ILD on HRCT, while only 27 (26.0%) had an FVC 80% of predicted, and 54 (53.0%) had a decrease in the results of at least 1 PFT. Forty (62.5%) of 64 patients with significant ILD on HRCT had a normal FVC value, translating into a high false-negative rate. Notably, 5 of 40 patients with a normal FVC value had severe, functionally occult lung fibrosis; in 2 of these patients, the results of all of the PFTs were within normal limits. Patients with normal FVC values despite evidence of fibrosis on HRCT more frequently had anti–Scl-70 antibodies and diffuse SSc and less frequently had anticentromere antibodies (ACAs) compared with patients with both normal FVC values and normal HRCT results. Conclusion The derived evidence-based data reveal a high risk of missing significant SSc-related ILD when relying solely on PFTs. More comprehensive screening algorithms for early detection are warranted. In particular, additional imaging investigations for the early detection of SSc-related ILD should be considered in ACA-negative patients with normal FVC values.