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Genetic variation in stem characters and their relation to 1000 kernel weight and spike yield in wheat under abiotic stresses

Research Abstract
The frequency, duration, and severity of heat and drought stresses singly or in combination can be aggravated by climate change. Under these stresses, the stored carbohydrates in stems play an important role in grain filling in wheat (Triticum aestivum L.) post-anthesis. This study examines the genetic variation of stem characters in wheat and their role in supporting grain weight under favorable and drought, combined drought, and heat conditions. Field experiments were conducted with seven spring wheat genotypes along with their F1 and F2 progenies. Stem diameter (SD), stem density (SDN), and stem weight (SW) were measured in 21 F1 (half-diallel analysis) and 12 F2 (3 x 4 North Carolina Design II) crosses. Among stem characters, SD was significantly correlated with thousand kernel weight (TKW) and grain yield per spike (GYPS) in all environments in F1 and F2. All characters showed high heritability estimates. For stem characters, the highest heritability was found for SD in both generations. High positive phenotypic and genotypic correlations were found between stem characters and both TKW and GYPS. The correlation between reductions due to stress (%) in stem characters and reduction due to stress in TKW and GYPS confirmed the strong relationships between stem characters and yield attributes in the stressed environments. We conclude that stem characters, especially SD, may play an important role in sustaining grain filling under such conditions. This is possibly due to a greater stem capacity for assimilates storage post-anthesis and subsequent remobilization to the grains
Research Authors
Sallam, A., M. Hashad, EL. Hamed, M. Kadry
Research Department
Research Journal
Journal of Crop Science and Biotechnology
Research Member
Research Pages
137-146
Research Publisher
Springer
Research Rank
1
Research Vol
18 - 3
Research Website
http://link.springer.com/article/10.1007%2Fs12892-015-0014-z
Research Year
2015

Genetic variation in stem characters and their relation to 1000 kernel weight and spike yield in wheat under abiotic stresses

Research Abstract
The frequency, duration, and severity of heat and drought stresses singly or in combination can be aggravated by climate change. Under these stresses, the stored carbohydrates in stems play an important role in grain filling in wheat (Triticum aestivum L.) post-anthesis. This study examines the genetic variation of stem characters in wheat and their role in supporting grain weight under favorable and drought, combined drought, and heat conditions. Field experiments were conducted with seven spring wheat genotypes along with their F1 and F2 progenies. Stem diameter (SD), stem density (SDN), and stem weight (SW) were measured in 21 F1 (half-diallel analysis) and 12 F2 (3 x 4 North Carolina Design II) crosses. Among stem characters, SD was significantly correlated with thousand kernel weight (TKW) and grain yield per spike (GYPS) in all environments in F1 and F2. All characters showed high heritability estimates. For stem characters, the highest heritability was found for SD in both generations. High positive phenotypic and genotypic correlations were found between stem characters and both TKW and GYPS. The correlation between reductions due to stress (%) in stem characters and reduction due to stress in TKW and GYPS confirmed the strong relationships between stem characters and yield attributes in the stressed environments. We conclude that stem characters, especially SD, may play an important role in sustaining grain filling under such conditions. This is possibly due to a greater stem capacity for assimilates storage post-anthesis and subsequent remobilization to the grains
Research Authors
Sallam, A., M. Hashad, EL. Hamed, M. Kadry
Research Department
Research Journal
Journal of Crop Science and Biotechnology
Research Pages
137-146
Research Publisher
Springer
Research Rank
1
Research Vol
18 - 3
Research Website
http://link.springer.com/article/10.1007%2Fs12892-015-0014-z
Research Year
2015

Genetic variation in stem characters and their relation to 1000 kernel weight and spike yield in wheat under abiotic stresses

Research Abstract
The frequency, duration, and severity of heat and drought stresses singly or in combination can be aggravated by climate change. Under these stresses, the stored carbohydrates in stems play an important role in grain filling in wheat (Triticum aestivum L.) post-anthesis. This study examines the genetic variation of stem characters in wheat and their role in supporting grain weight under favorable and drought, combined drought, and heat conditions. Field experiments were conducted with seven spring wheat genotypes along with their F1 and F2 progenies. Stem diameter (SD), stem density (SDN), and stem weight (SW) were measured in 21 F1 (half-diallel analysis) and 12 F2 (3 x 4 North Carolina Design II) crosses. Among stem characters, SD was significantly correlated with thousand kernel weight (TKW) and grain yield per spike (GYPS) in all environments in F1 and F2. All characters showed high heritability estimates. For stem characters, the highest heritability was found for SD in both generations. High positive phenotypic and genotypic correlations were found between stem characters and both TKW and GYPS. The correlation between reductions due to stress (%) in stem characters and reduction due to stress in TKW and GYPS confirmed the strong relationships between stem characters and yield attributes in the stressed environments. We conclude that stem characters, especially SD, may play an important role in sustaining grain filling under such conditions. This is possibly due to a greater stem capacity for assimilates storage post-anthesis and subsequent remobilization to the grains
Research Authors
Sallam, A., M. Hashad, EL. Hamed, M. Kadry
Research Department
Research Journal
Journal of Crop Science and Biotechnology
Research Pages
137-146
Research Publisher
Springer
Research Rank
1
Research Vol
18 - 3
Research Website
http://link.springer.com/article/10.1007%2Fs12892-015-0014-z
Research Year
2015

Genetic variation in stem characters and their relation to 1000 kernel weight and spike yield in wheat under abiotic stresses

Research Abstract
The frequency, duration, and severity of heat and drought stresses singly or in combination can be aggravated by climate change. Under these stresses, the stored carbohydrates in stems play an important role in grain filling in wheat (Triticum aestivum L.) post-anthesis. This study examines the genetic variation of stem characters in wheat and their role in supporting grain weight under favorable and drought, combined drought, and heat conditions. Field experiments were conducted with seven spring wheat genotypes along with their F1 and F2 progenies. Stem diameter (SD), stem density (SDN), and stem weight (SW) were measured in 21 F1 (half-diallel analysis) and 12 F2 (3 x 4 North Carolina Design II) crosses. Among stem characters, SD was significantly correlated with thousand kernel weight (TKW) and grain yield per spike (GYPS) in all environments in F1 and F2. All characters showed high heritability estimates. For stem characters, the highest heritability was found for SD in both generations. High positive phenotypic and genotypic correlations were found between stem characters and both TKW and GYPS. The correlation between reductions due to stress (%) in stem characters and reduction due to stress in TKW and GYPS confirmed the strong relationships between stem characters and yield attributes in the stressed environments. We conclude that stem characters, especially SD, may play an important role in sustaining grain filling under such conditions. This is possibly due to a greater stem capacity for assimilates storage post-anthesis and subsequent remobilization to the grains
Research Authors
Sallam, A., M. Hashad, EL. Hamed, M. Kadry
Research Department
Research Journal
Journal of Crop Science and Biotechnology
Research Pages
137-146
Research Publisher
Springer
Research Rank
1
Research Vol
18 - 3
Research Website
http://link.springer.com/article/10.1007%2Fs12892-015-0014-z
Research Year
2015

Genetic variation of morpho-physiological traits associated with frost tolerance in faba bean

Research Abstract
Frost stress is one of the most significant abiotic stresses affecting crops in Europe and limits e.g. winter faba bean growth. Faba bean is therefore mainly sown as a spring crop in cool-temperate regions. The objective of this study is to study the genetic variation in frost tolerance between elite winter faba beans. To address this challenge, 200 highly homozygous lines were tested in a frost growth chamber. Two different experiments, with frost stress and with hardening conditions were conducted. A number of morphological traits of shoots (loss of leaf turgidity, loss of leaf color, regrowth after frost and disposition to survive) and of roots (root frost susceptibility and root length) were visually scored in the frost experiments. Moreover, total leaf fatty acid (after hardening) and water content (after hardening and after frost stress) in shoots as physiological parameters were analyzed. The frost tolerance index was calculated to improve two traits: disposition to survive and regrowth after frost. High significant genetic variation was found between genotypes in all traits scored and analyzed. Disposition to survive showed high significant phenotypic (r = 0.77**) and genotypic (r = 0.85) correlations with regrowth after frost test. Moreover, roots traits showed higher significant phenotypic and genotypic correlations with frost tolerance than traits scored on shoots (loss of leaf turgidity and loss of leaf color). For physiological traits, water content before and after frost showed stronger significant correlation with frost tolerance than leaf fatty acid composition. Repeatability estimates (h2) ranged from 0.58 to 0.95 for morphological traits and from 0.63 to 0.89 for physiological traits. The best 20 genotypes for each morphological trait were determined. As a result, 12 genotypes were identified with superior frost tolerance. The results indicate the possibility of genetically improving the frost tolerance in winter faba bean through selection programs.
Research Authors
Sallam, A., R. Martsch, Y. Moursi
Research Department
Research Journal
Euphytica
Research Member
Research Pages
395-407
Research Publisher
Springer
Research Rank
1
Research Vol
205
Research Website
http://link.springer.com/article/10.1007%2Fs10681-015-1395-2#/page-1
Research Year
2015

The transgenerational impact of benzo(a)pyrene on murine male fertility.

Research Abstract
Abstract BACKGROUND: Benzo(a)pyrene (BaP) is an endocrine toxicant that is widely distributed in the environment. The adverse effects of BaP on fertility are well documented, however its effects on fertility in the subsequent generations are not known. We aimed to investigate the transgenerational effects of BaP on male fertility in mice. METHODS: Six-week-old male mice (F0) were orally administered BaP (1 or 10 mg/kg body weight) or corn oil, daily for 6 weeks. The male mice were mated with untreated female mice to produce F1 offspring. The F2 and F3 progeny were produced in a similar manner. Testes and spermatozoa were collected from 14-week-old F0, F1, F2 and F3 males in order to assess male fertility parameters, namely testis histology, sperm count, sperm motility and sperm penetration (sperm penetration assay). RESULTS: Oral administration of a high dose of BaP induced testicular malformation and decreased numbers of seminiferous tubules with elongated spermatids for three generations studied (i.e. F0 to F2) with significant decreases in F0 and F2. It also significantly decreased sperm motility in F0. BaP significantly decreased sperm count in the group treated with a high dose of BaP in all generations except the F3 generation. The sperm fertility index (SFI) also decreased significantly for two generations. Of the fertility parameters measured, sperm count and SFI were the more sensitive parameters in our study. CONCLUSIONS: Exposure to BaP decreases the fertilization potential of exposed males and has an adverse impact on sperm function and fertility in subsequent generations. The BaP effect on fertility can be described as a transgenerational effect for F2 generation.
Research Authors
El-Sayed A. Mohamed, Won-Hee Song, Shin-Ae Oh, Yoo-Jin Park, Young-Ah You, Sangmi Lee, Ji-Young Choi, Young-Ju Kim, Inho Jo, Myung-Geol Pang.
Research Department
Research Journal
Human Reproduction
Research Pages
2427-2433.
Research Rank
1
Research Vol
25
Research Year
2010

Voltage-dependent anion channels are a key factor of male fertility

Research Abstract
Abstract OBJECTIVE: To examine how voltage-dependent anion channels (VDACs) regulate sperm function in capacitation conditions. DESIGN: Experimental prospective study. SETTING: Academic research laboratory. ANIMAL(S): Male ICR and female B6D2F1/CrljOri mice (8-12 weeks old). INTERVENTION(S): Female mice were superovulated with 5 IU of pregnant mare serum gonadotropin given IP and 5 IU of hCG given IP 48 hours later. Oocytes were applied to assess fertilization and embryo development. MAIN OUTCOME MEASURE(S): Immunofluorescence assay, computer-assisted sperm analysis, hypo-osmotic swelling test, combined Hoechst 33258/chlortetracycline fluorescence assessment of capacitation status, measurement of [Ca(2+)](i) and [pH](i), Western blotting, and IVF. RESULT(S): VDAC2 was localized on the acrosomal region and principal piece, while VDAC3 was localized on the acrosomal region and midpiece. Blocking VDAC with DIDS (500 μM) significantly decreased motility, viability, acrosome reaction, capacitation, tyrosine phosphorylation, fertilization, and embryo development regardless of Ca(2+). However, the most severe decreases were observed in the presence (+) of DIDS and absence (-) of Ca(2+), respectively. A significant decrease in [Ca(2+)](i) concentration was observed in (-) DIDS, while [pH](i) was significantly increased in (-) DIDS regardless of Ca(2+). However, a significantly elevated [pH](i) was observed in (+) Ca(2+). CONCLUSION(S): Abnormal regulation of VDACs negatively affected sperm function. Thus, VDACs may be key regulators of the fertilization ability of spermatozoa.
Research Authors
2- Woo-Sung Kwon, Yoo-Jin Park, El-Sayed A. Mohamed, Myung-Geol Pang.
Research Department
Research Journal
Fertility and Sterility
Research Pages
354-361
Research Rank
1
Research Vol
99
Research Year
2013

Effect of duration of feed withdrawal versus ad libitum feeding during high environmental temperature on broiler chicken performance.

Research Abstract
This experiment was conducted to investigate the effect of duration of feed withdrawal and full-feeding during high environmental temperature condition on broiler chicken performance and mortality rate. One hundred and twenty one-day old brown Shaver broilers were used in this study. The experiment consisted of four groups, each group included three replicates of 10 chicks each. The broilers were full-fed ad libitum in the control group 1 (FFC) while feeders were removed for three (at 9.00 a.m. and return it at 12.00 p.m.), six (at 9.00 a.m. and return it at 3.00 p.m.) or nine (at 9.00 a.m. and return it at 6.00 p.m.) hours per day in groups 2, 3 and 4, (FW3, FW6, and FW9) respectively from 2 to 7 weeks of age. The average indoor temperature was 26-38 °C. The obtained results could be summarized as follows: No significant differences in the final body weight, feed consumption and cumulative feed conversion ratio were observed between full-fed and all restricted broilers. The broilers of all restricted-fed groups had fewer deaths than that of the control group. The mortality rate was 13.3, 10.0, 0.0 and 10.0% for groups 1, 2, 3, and 4, respectively. No significant differences in carcass weight, its percentage, as well as the percentage of femurs, breast, wings, blood, feet & shank, heart, liver, gizzard, giblets, intestine, drumsticks & femurs fat, and total fat weight were found among all groups. However, significant (P≤0.05) differences were detected in the percentage of head, back, skin, proventriculus, spleen, gallbladder, abdominal fat, subcutaneous fat and breast fat of the groups. The broilers of all restricted-fed groups had significantly lower (P≤0.05) percentage of drumsticks & femurs as compared with that of FFC. It could be observed that, the FFC group had significantly higher (P≤0.05) neck fat as compared with all restricted groups, while it had significantly lower (P≤0.05) heart fat. The FW6 birds had insignificantly lower total fat than that of FFC birds. All restricted fed broilers (groups 2, 3 and 4) increased economical efficiency by 13, 99 and 2%, respectively as compared with FFC broilers. The FW6 group had the best economic efficiency (EE) value. It could be concluded that, the most suitable feeding regime during high environmental temperature is the withdrawal of feed for 6 hours per day at 9.00 a.m. and return it at 3.00 p.m. from 2 to 7 weeks of age because it was best economically efficient than other feed restriction regimes. Also, using this regime was associated with a reduction in fat deposition and with no mortalities.
Research Authors
El-Sagheer M., and Makled M.N.,
Research Department
Research Journal
Egyptian Poultry Science Journal,
Research Pages
333-350.
Research Rank
2
Research Vol
Vol. 25, (II):
Research Year
2005

Effect of daily feeding time restriction on broiler chicken performance under summer season conditions of Upper Egypt.

Research Abstract
A total number of one hundred and fifty of one-day old brown shaver broilers chicks were used to study the effect of daily feeding time restriction on broiler chicken performance and mortality rate under summer season condition of Upper Egypt. The chicks were randomly divided into 5 experimental groups, (control and 4 treatments). Each group included three replicates of 10 chicks. Group 1 (FFC) was used as control in which the birds were fed ad libitum from 1 to 49 days of age. In groups 2 (EFR6) and 3 (EFR9), the feed was removed for 6 or 9 hours per day from 2 to 4 weeks of age, respectively. In groups 4 (LFR6) and 5 (LFR9), the feed was removed for 6 or 9 hours per day from 5 to 7 weeks of age, respectively. The birds were subjected to heat stress during the experiment since the temperature ranged between 26 and 38 °C. The obtained results could be summarized as follows: At 7 weeks of age, broilers of EFR6 group had significantly (P≤0.05) higher body weight (BW) than those of FFC, EFR9 and LFR9 groups, while the broilers of LFR6 group had an intermediate BW. Also, the broilers of EFR6 group showed a significantly higher (P≤0.05) daily weight gain than those of FFC, EFR9 and LFR9 groups, but not than those of LFR6 group. Time of feed restriction had no effect on the overall mean of feed consumption (FC) and in cumulative feed conversion ratio (FCR) among all groups, however birds in LFR-6H, EFR6 and EFR9 groups had better cumulative FCR by about 5.4, 3.6 and 2.3 %, respectively than that of FFC group. The broilers of all restricted fed groups had fewer deaths than their controls, while the mortality rate was 13.3, 0.00, 6.70, 0.00 and 6.70% for FFC, EFR6, EFR9, LFR6 and LFR9 groups, respectively. Broilers of EFR6 and EFR9 groups had significantly heavier (P≤0.05) carcass weight compared to those of FFC, LFR6 and LFR9 groups. It was found that the birds of EFR9 had significantly heavier breast (P≤0.05) percentage than those of LFR6 and LFR9, while FFC and EFR6 groups had an intermediate percentages. The broilers of EFR6 had significantly lower (P≤0.05) percentage of liver than those of FFC and LFR9; gizzard and giblets than those of FFC, LFR6 and LFR9, but there are significant (P≤0.05) differences in the fat percentages of abdominal, subcutaneous, neck and total of those groups. The EFR6 and EFR9 groups had insignificantly lower percentage of total fat by about 21.1 and 19.9%, respectively than that of FFC group. Time of feed restriction at different ages had no significant effect on the percentage of other carcass (yield and parts) and body organs weight. All restricted fed groups (EFR6, EFR9, LFR6 and LFR9) showed increased economical efficiency by 70, 36, 85 and 28%, respectively as compared with FFC one. It could be concluded that, the most suitable and economically efficient feeding program during high environmental temperature was feed restriction for 6 hours per day (from 9 a.m. to 3 p.m.) from 5 to 7 weeks of age.
Research Authors
El-Sagheer M., and Makled M.N.,
Research Department
Research Journal
Egyptian Poultry Science Journal,
Research Pages
863-877
Research Rank
2
Research Vol
(Vol. 25, (III)
Research Year
2005

أثر السياسة السعرية الزراعية على إنتاج وإستهلاك محصول القمح فى محافظة أسيوط بإستخدام نموذج التوازن الجزئى

Research Abstract
وقد استهدف هذا البحث قياس اثر تطبيق سياسة الإصلاح الاقتصادى على محصول القمح لكونه المحصول الأكبر مساحة فى محافظة أسيوط من خلال تطبيق نموذج التوازن الجزئى لتقدير صافى خسارة أو مكسب كل من المنتج والمستهلك الإقتصادية والتغير فى كل من فائض المنتج والمستهلك والإيراد الحكومى وحصيلة النقد الأجنبى. إعتمد البحث فى الحصول على بياناته على البيانات الإحصائية المنشورة وغير المنشورة التى تصدرها الإدارة المركزية للإقتصاد الزراعى بقطاع الشئون الإقتصادية التابع لوزارة الزراعة وإستصلاح الأراضى, والجهاز المركزى للتعبئة العامة والإحصاء, ومركز المعلومات ودعم إتخاذ القرار بمحافظة أسيوط. إعتمد البحث لتحقيق أهدافه على إسلوبى التحليل الوصفى والكمى من خلال إستخدام بعض الأساليب الرياضية والإحصائية المختلفة, ولتحقيق هدف البحث فقد تم تقسيم فترة الدراسة إلى ثلاث فترات هى فترة ما قبل تطبيق سياسات التحرر الإقتصادى (1980 – 1986), وفترة التحرر الجزئى (1987 – 1992), وفترة التحرر الكامل لقطاع الزراعة (1993 – 2010). وتبين النتائج أن الحد الثابت للمعادلة (الجزء المقطوع من المحور الرأسى) حدث له تغير ثبت معنويته احصائياً قدر بحوالى 111.16, 80.64, 71.11 ألف فدان للمساحة المزروعة, وحوالى 8.60, 14.75, 14.10 أردب للإنتاجية الفدانية, وحوالى 969.65, 1204.40, 739.56 ألف أردب للإنتاج الكلى لمحصول القمح لكل من الفترات الثلاث السابق الاشارة إليها على الترتيب, حيث يشير ذلك أن لكل معدل نمو سنوى اصبح هناك مستوى اعلى لمتغيرات الدراسة حيث يوضح ذلك اثر التحرر الاقتصادى على التغير الهيكلى فى متغيرات الدراسة السابق الاشارة إليها, وتبين النتائج أيضاً أن هناك تغير سنوى ثبت معنويته احصائياً قدر بحوالى -3.33 ألف فدان للمساحة المزروعة لمحصول القمح خلال الفترة الأولى. وعند تقدير معامل ومعدل الحماية الإسمى لمحصول القمح بالاسعار المثبتة فى محافظة أسيوط, تبين أن منتجى القمح قد حصلوا على حوالى 43%, 84%,40% من قيمة ناتجهم فى حالة بيعة بالسعر العالمى وذلك خلال الفترات الثلاث السابق الاشارة إليها على الترتيب, ومن نتائج نموذج التوازن الجزئى لمحصول القمح عند استخدام الأسعار المثبتة فى محافظة أسيوط تبين أن صافى خسارة المنتج الإقتصادية بلغ حوالى 11.35, 8.46, 197.48 مليون جنية, وأن صافى مكسب المستهلك الاقتصادى بلغ حوالى 14.99, 17.43, 316.33 مليون جنية, وبلغت أيضاً الخسارة فى فائض المنتج حوالى 34.98, 33.64, 454.50 مليون جنية, وكما تبين أن المكسب فى فائض المستهبلك بلغ حوالى 72.76, 59.04, 627.43 مليون جنية, وتبين أن خسارة الحكومة بلغت حوالى 34.14, 16.43, 54.08 مليون جنية, كما يتضح أنه تحقق مكسب فى حصيلة النقد الأجنبى بلغ حوالى 13.52, 51.68, 351.19 مليون جنية, وتبين أيضاً أن صافى المكسب الإقتصادى (صافى الأثر على الواردات) بلغ حوالى 3.65, 8.98, 118.85 مليون جنية وذلك من خلال الفترات الثلاث السابق الاشارة إليها على الترتيب. هذا وقد أوصت الدراسة بما يلى: 1- ضرورة العمل على ضمان حدود دنيا لأسعار التوريد المقدمة لمحصول القمح بحيث يتناسب فيها السعر المحلى مع السعر العالمى, مع الإعلان عنه قبل موعد الزراعة بوقت كافى حتى يسترشد بها المزارعين عند اتخاذ قراراتهم مما قد يشجعهم على التوسع فى زراعة هذا المحصول. 2- الإستمرار فى مراجعة وتعديل برنامج الإصلاح الإقتصادى خاصة فيما يتعلق بمعالجة الأثار السلبية للبرنامج والخاصة بتخفيض الضرائب المفروضة على المنتجين الزراعيين والتى تؤدى زيادتها إلى زيادة صافى الخسارة الإقتصادية فى إنتاج محصول القمح.
Research Authors
أ.د/على عبد الجليل عيسى, أ.د/أحمد عبد الحفيظ محمد
أحمد محمود عبد العزيز, السيد طه/ فاروق محمد البرعى
Research Journal
مجلة أسيوط للعلوم الزراعي
Research Pages
134 - 154
Research Publisher
كلية الزراعة، جامعة أسيوط
Research Rank
2
Research Vol
المجلد (43) العدد 4
Research Year
2012
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