GBM, the most common primary malignant brain tumor in adults, has an overall dismal prognosis. Immunotherapy targeting the PD-1/PD-L1 axis has shown limited success in GBM. Resistance to therapies involves different pathways like autophagy. Detecting LC3B expression provides a simple technique for monitoring autophagy. Our goal was to understand the interplay between PD-L1 and LC3B in GBM prognosis and treatment strategies. The study analyzed 61 GBM specimens to assess the immunohistochemical expression of PD-L1 and LC3B with investigating their correlation with various clinicopathological parameters with assessing the impact of PD-L1 and LC3B expression on patients’ survival and the relation between both markers. Both PD-L1 and LC3B were significantly associated with clinicopathological parameters, including Karnofsky performance score (KPS)(P = 0.028 and 0.004 respectively …