Objectives: To evaluate the prognostic role of post-therapy 99mTc-DMSA-V brain SPECT/CT scans in patients with glioma. Methods: Patients with glioma underwent post-treatment SPECT/CT scanning using 99mTc-DMSA-V in this prospective study. Scan results were reported as positive or negative for tumor residual/recurrence. Three -year overall survival (OS) was calculated from the date of diagnosis to the date of death or last follow-up. Results: A total of 37 patients were eligible for analysis with mean survival of 37.5 months (range = 28.6 – 46.5). At the end of follow-up, 13 patients died (3-year OS = 42%). Ten out of 17 patients with positive DMSA-V scan findings died compared to 3 out of 20 patients with negative scans. Three-year OS were 13% for the positive group with mean survival of 22.2 months compared to 74% and mean survival of 50.3 months for the negative group (P = 0.003). Additionally, patients with negative scans survived significantly longer than positive group after their date of scanning (mean survival 20.7 months vs. 11.0 months; P = 0.001). DMSA-V scan results were still significant predictor of OS after adjusting for age, gender and pathology in Cox proportional hazard model (P = 0.03; Hazards ratio [HR] = 4.8 [95% CI = 1.2 – 19.7]). However, DMSA-V scan results were strongly associated with Karnofsky performance scale (KPS) (P = 0.002). A scoring system which includes both DMSA-V scan results and KPS (cut-off 80) identified 3 different groups with significant difference in OS; score 0 = negative scan & KPS 蠅 80 (reference group), score 1 = positive scan or KPS 80 and score 2 = positive scan with KPS 80 (P = 0.006). Patients with score 2 have HR of 11.4 compared to 3.8 for score 1. Conclusion: Post-therapy SPECT/CT scanning with 99mTc-DMSA-V in patients with glioma is a potential prognostic factor for OS and may be helpful in risk stratification of glioma patients after their treatment. Supporting graph: Supporting Tables

Background and aim of work: We aimed from this study to determine the response and local relapse free survival (RFS) of pediatric patients with localized Ewing’s sarcoma treated with accelerated hyper-fractionated RT. Patient and methods: This study was a nonrandomized uncontrolled phase II study and was conducted at clinical oncology department and south Egypt cancer institute; it involved 28 patients with histologically confirmed Ewing’s sarcoma; all of them were subjected to PET/CT whenever possible or MRI with contrast of the primary site, MSCT chest, bone scan, and LDH to ensure absence of metastasis followed by the protocol of accelerated hyper fractionated RT. Results: The overall response rate (ORR) was 92.9% by MRI with significant effect of the type of response on local RFS (P 0.002). The median local RFS of 28 patients with localized Ewing’s sarcoma was 30 ± 8.599 months with 95% CI = 13.147 - 46.853; the 3-year local control was 35%. Conclusion: Accelerated hyper fractionated RT didn’t achieve better results than standard fractionation RT, but it is recommended to be done on a large sample size, and multiple centers, and continued follow up is also recommended to evaluate 5-year LRFS, 5-year OS.

Background: Tuberculosis (TB) has been a major health problem in developing countries. Rapid diagnosis of Mycobacterium tuberculosis infection plays a critical role in controlling the spread of tuberculosis. Conventional methods may take up to several weeks or longer to produce results. This study aimed to compare different methods like culture on Bio F-M media, automated BacT/ ALERT MP bottles, and urine lipoarabinomnnan by ELISA. Subjects and Methods: This study was done on 100 patients suspected to have tuberculosis, cases divided into two groups: (Group A): 68 clinically suspected pulmonary TB cases. (Group B): 32 clinically suspected extrapulmonary TB cases. All samples from Group A and B were subjected to direct staining by Zeihl-Neelsen stain, culture of the samples on Lowenstein Jensen media, Bio FM media and automated BacT/ALERT MP bottles and detection of urine lipoarabinomannan by ELISA. Results: In suspected pulmonary tuberculosis cases the sensitivity and specificity of Bio FM were 87.2%, 100% respectively, sensitivity and specificity of BacT/ALERT MP were 74.4%, 100% respectively, sensitivity and specificity of urine lipoarabinomannan were 20.5%, 96.9% respectively and in suspected extrapulmonary tuberculosis cases were the sensitivity and specificity of Bio FM were 87.5%, 100% respectively, sensitivity and specificity of BacT/ALERT MP were 50%, 100% respectively, sensitivity and specificity of urine lipoarabinomannan were 12.5%, 100% respectively. Conclusion: The sensitivity of both solid (Lowenstein Jensen) and liquid (Bio FM) media were the same and better than BacT/ALERT MP. Bio FM media and BacT/ALERT MP show shorter time than Lowenstein Jensen for detection of mycobacterial growth. Detection of urine lipoarabinomannan by ELISA is insensitive for the diagnosis of TB.

Background: Tuberculosis (TB) has been a major health problem in developing countries. Rapid diagnosis of Mycobacterium tuberculosis infection plays a critical role in controlling the spread of tuberculosis. Conventional methods may take up to several weeks or longer to produce results. This study aimed to compare different methods like culture on Bio F-M media, automated BacT/ ALERT MP bottles, and urine lipoarabinomnnan by ELISA. Subjects and Methods: This study was done on 100 patients suspected to have tuberculosis, cases divided into two groups: (Group A): 68 clinically suspected pulmonary TB cases. (Group B): 32 clinically suspected extrapulmonary TB cases. All samples from Group A and B were subjected to direct staining by Zeihl-Neelsen stain, culture of the samples on Lowenstein Jensen media, Bio FM media and automated BacT/ALERT MP bottles and detection of urine lipoarabinomannan by ELISA. Results: In suspected pulmonary tuberculosis cases the sensitivity and specificity of Bio FM were 87.2%, 100% respectively, sensitivity and specificity of BacT/ALERT MP were 74.4%, 100% respectively, sensitivity and specificity of urine lipoarabinomannan were 20.5%, 96.9% respectively and in suspected extrapulmonary tuberculosis cases were the sensitivity and specificity of Bio FM were 87.5%, 100% respectively, sensitivity and specificity of BacT/ALERT MP were 50%, 100% respectively, sensitivity and specificity of urine lipoarabinomannan were 12.5%, 100% respectively. Conclusion: The sensitivity of both solid (Lowenstein Jensen) and liquid (Bio FM) media were the same and better than BacT/ALERT MP. Bio FM media and BacT/ALERT MP show shorter time than Lowenstein Jensen for detection of mycobacterial growth. Detection of urine lipoarabinomannan by ELISA is insensitive for the diagnosis of TB.

Background: Tuberculosis (TB) has been a major health problem in developing countries. Rapid diagnosis of Mycobacterium tuberculosis infection plays a critical role in controlling the spread of tuberculosis. Conventional methods may take up to several weeks or longer to produce results. This study aimed to compare different methods like culture on Bio F-M media, automated BacT/ ALERT MP bottles, and urine lipoarabinomnnan by ELISA. Subjects and Methods: This study was done on 100 patients suspected to have tuberculosis, cases divided into two groups: (Group A): 68 clinically suspected pulmonary TB cases. (Group B): 32 clinically suspected extrapulmonary TB cases. All samples from Group A and B were subjected to direct staining by Zeihl-Neelsen stain, culture of the samples on Lowenstein Jensen media, Bio FM media and automated BacT/ALERT MP bottles and detection of urine lipoarabinomannan by ELISA. Results: In suspected pulmonary tuberculosis cases the sensitivity and specificity of Bio FM were 87.2%, 100% respectively, sensitivity and specificity of BacT/ALERT MP were 74.4%, 100% respectively, sensitivity and specificity of urine lipoarabinomannan were 20.5%, 96.9% respectively and in suspected extrapulmonary tuberculosis cases were the sensitivity and specificity of Bio FM were 87.5%, 100% respectively, sensitivity and specificity of BacT/ALERT MP were 50%, 100% respectively, sensitivity and specificity of urine lipoarabinomannan were 12.5%, 100% respectively. Conclusion: The sensitivity of both solid (Lowenstein Jensen) and liquid (Bio FM) media were the same and better than BacT/ALERT MP. Bio FM media and BacT/ALERT MP show shorter time than Lowenstein Jensen for detection of mycobacterial growth. Detection of urine lipoarabinomannan by ELISA is insensitive for the diagnosis of TB.

Background: Tuberculosis (TB) has been a major health problem in developing countries. Rapid diagnosis of Mycobacterium tuberculosis infection plays a critical role in controlling the spread of tuberculosis. Conventional methods may take up to several weeks or longer to produce results. This study aimed to compare different methods like culture on Bio F-M media, automated BacT/ ALERT MP bottles, and urine lipoarabinomnnan by ELISA. Subjects and Methods: This study was done on 100 patients suspected to have tuberculosis, cases divided into two groups: (Group A): 68 clinically suspected pulmonary TB cases. (Group B): 32 clinically suspected extrapulmonary TB cases. All samples from Group A and B were subjected to direct staining by Zeihl-Neelsen stain, culture of the samples on Lowenstein Jensen media, Bio FM media and automated BacT/ALERT MP bottles and detection of urine lipoarabinomannan by ELISA. Results: In suspected pulmonary tuberculosis cases the sensitivity and specificity of Bio FM were 87.2%, 100% respectively, sensitivity and specificity of BacT/ALERT MP were 74.4%, 100% respectively, sensitivity and specificity of urine lipoarabinomannan were 20.5%, 96.9% respectively and in suspected extrapulmonary tuberculosis cases were the sensitivity and specificity of Bio FM were 87.5%, 100% respectively, sensitivity and specificity of BacT/ALERT MP were 50%, 100% respectively, sensitivity and specificity of urine lipoarabinomannan were 12.5%, 100% respectively. Conclusion: The sensitivity of both solid (Lowenstein Jensen) and liquid (Bio FM) media were the same and better than BacT/ALERT MP. Bio FM media and BacT/ALERT MP show shorter time than Lowenstein Jensen for detection of mycobacterial growth. Detection of urine lipoarabinomannan by ELISA is insensitive for the diagnosis of TB.

NULL

NULL

NULL

NULL