Background: Giardia lamblia is one of the most common protozoal infections in human especially children. Metronidazol (MTZ) is the drug of choice for treatment of giardiasis; its chemical composition possesses major threats and is becoming less sensitive. This study aimed to search for natural extracts alternative to MTZ. Methods: In-vivo effects of dichloromethane extracts of ginger and cinnamon in doses of 10 and 20 mg/kg/day separately were studied on 30 experimentally infected albino rats divided into 6 groups (5 rats each). Plant extracts were started on the 6th day post infection for 7 successive days. The study was evaluated by fecal cyst and intestinal trophozoite counts, histopathology, scanning and transmission electron microscopic examinations of the small intestinal mucosa. Results: Ginger and cinnamon caused reduction of fecal cyst and trophozoites counts. Histopathology, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) after exposure to each extract revealed evident improvement of intestinal mucosal damage produced by G. lamblia infection and direct structural injury to the trophozoites. However, these results were more obvious after exposure to cinnamon extracts. Conclusion: We confirmed the potential therapeutic effects of ginger and cinnamon extracts on G. lamblia infection in albino rats as a promising alternative therapy to the commonly used antigiardial drugs

Background: Giardia lamblia is one of the most common protozoal infections in human especially children. Metronidazol (MTZ) is the drug of choice for treatment of giardiasis; its chemical composition possesses major threats and is becoming less sensitive. This study aimed to search for natural extracts alternative to MTZ. Methods: In-vivo effects of dichloromethane extracts of ginger and cinnamon in doses of 10 and 20 mg/kg/day separately were studied on 30 experimentally infected albino rats divided into 6 groups (5 rats each). Plant extracts were started on the 6th day post infection for 7 successive days. The study was evaluated by fecal cyst and intestinal trophozoite counts, histopathology, scanning and transmission electron microscopic examinations of the small intestinal mucosa. Results: Ginger and cinnamon caused reduction of fecal cyst and trophozoites counts. Histopathology, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) after exposure to each extract revealed evident improvement of intestinal mucosal damage produced by G. lamblia infection and direct structural injury to the trophozoites. However, these results were more obvious after exposure to cinnamon extracts. Conclusion: We confirmed the potential therapeutic effects of ginger and cinnamon extracts on G. lamblia infection in albino rats as a promising alternative therapy to the commonly used antigiardial drugs

Background: Giardia lamblia is one of the most common protozoal infections in human especially children. Metronidazol (MTZ) is the drug of choice for treatment of giardiasis; its chemical composition possesses major threats and is becoming less sensitive. This study aimed to search for natural extracts alternative to MTZ. Methods: In-vivo effects of dichloromethane extracts of ginger and cinnamon in doses of 10 and 20 mg/kg/day separately were studied on 30 experimentally infected albino rats divided into 6 groups (5 rats each). Plant extracts were started on the 6th day post infection for 7 successive days. The study was evaluated by fecal cyst and intestinal trophozoite counts, histopathology, scanning and transmission electron microscopic examinations of the small intestinal mucosa. Results: Ginger and cinnamon caused reduction of fecal cyst and trophozoites counts. Histopathology, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) after exposure to each extract revealed evident improvement of intestinal mucosal damage produced by G. lamblia infection and direct structural injury to the trophozoites. However, these results were more obvious after exposure to cinnamon extracts. Conclusion: We confirmed the potential therapeutic effects of ginger and cinnamon extracts on G. lamblia infection in albino rats as a promising alternative therapy to the commonly used antigiardial drugs

Background: Giardia lamblia is one of the most common protozoal infections in human especially children. Metronidazol (MTZ) is the drug of choice for treatment of giardiasis; its chemical composition possesses major threats and is becoming less sensitive. This study aimed to search for natural extracts alternative to MTZ. Methods: In-vivo effects of dichloromethane extracts of ginger and cinnamon in doses of 10 and 20 mg/kg/day separately were studied on 30 experimentally infected albino rats divided into 6 groups (5 rats each). Plant extracts were started on the 6th day post infection for 7 successive days. The study was evaluated by fecal cyst and intestinal trophozoite counts, histopathology, scanning and transmission electron microscopic examinations of the small intestinal mucosa. Results: Ginger and cinnamon caused reduction of fecal cyst and trophozoites counts. Histopathology, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) after exposure to each extract revealed evident improvement of intestinal mucosal damage produced by G. lamblia infection and direct structural injury to the trophozoites. However, these results were more obvious after exposure to cinnamon extracts. Conclusion: We confirmed the potential therapeutic effects of ginger and cinnamon extracts on G. lamblia infection in albino rats as a promising alternative therapy to the commonly used antigiardial drugs

Schistosoma mansoni and fascioliasis are worldwide parasitic diseases infecting 83 and 17 million people respectively causing significant morbidity and mortality. The present work was carried out to evaluate the molluscicidal activities of the ethanolic extract of three plant species namely Euphorbia aphylla, Ziziphus spina-christi and Enterolobium contortisiliquum against Biomophalaria alexandrina and Lymnaea cailliaudi (natalensis), the snails intermediate hosts of Schistosoma mansoni and Fasciola respectively. The highest molluscicidal activity was recorded for Euphorbia aphylla on both snails’ species. The least activity was recorded for Enterolobium contortisiliquum which gave negative results against both snail species up to1000 ppm. The LC50 and LC90 of the ethanolic extract of Euphorbia aphylla against Biomophalaria alexandrina snails were 87.6 ppm and142.5ppm respectively while the LC50 and LC90 of Euphorbia aphylla against Lymnaea cailliaudi snails were 0.66 ppm and 0.88 ppm respectively. The LC50 and LC90 of Ziziphus spinachristi ethanolic extract against Lymnaea cailliaudi snails were 311ppm and 500 ppm respectively. However it showed no molluscicidal activity againist B. alexandrina snails up to 1000 ppm. The histopathological changes in hermaphrodite gland of B. alexandrina and Lymnaea cailliaudi snails 2 weeks post exposure to LC25 of the ethanolic extract Euphorbia aphylla and the histopathological effect of LC25 of the ethanolic extract Ziziphus spina- christi on Lymnaea cailliaudi snails were studied. Degenerative changes in hermaphrodite acini and their contents of ova and sperms were observed. The purification of active compounds present in Euphorbia aphylla and Ziziphus spina- christi plants may offers an alternative tool for the control of snails’ intermediate hosts of Schistosoma mansoni and Fasciola. Further investigations for Enterolobium contortisiliquum may increase its potential use as plant molluscicide are recommended

Schistosoma mansoni and fascioliasis are worldwide parasitic diseases infecting 83 and 17 million people respectively causing significant morbidity and mortality. The present work was carried out to evaluate the molluscicidal activities of the ethanolic extract of three plant species namely Euphorbia aphylla, Ziziphus spina-christi and Enterolobium contortisiliquum against Biomophalaria alexandrina and Lymnaea cailliaudi (natalensis), the snails intermediate hosts of Schistosoma mansoni and Fasciola respectively. The highest molluscicidal activity was recorded for Euphorbia aphylla on both snails’ species. The least activity was recorded for Enterolobium contortisiliquum which gave negative results against both snail species up to1000 ppm. The LC50 and LC90 of the ethanolic extract of Euphorbia aphylla against Biomophalaria alexandrina snails were 87.6 ppm and142.5ppm respectively while the LC50 and LC90 of Euphorbia aphylla against Lymnaea cailliaudi snails were 0.66 ppm and 0.88 ppm respectively. The LC50 and LC90 of Ziziphus spinachristi ethanolic extract against Lymnaea cailliaudi snails were 311ppm and 500 ppm respectively. However it showed no molluscicidal activity againist B. alexandrina snails up to 1000 ppm. The histopathological changes in hermaphrodite gland of B. alexandrina and Lymnaea cailliaudi snails 2 weeks post exposure to LC25 of the ethanolic extract Euphorbia aphylla and the histopathological effect of LC25 of the ethanolic extract Ziziphus spina- christi on Lymnaea cailliaudi snails were studied. Degenerative changes in hermaphrodite acini and their contents of ova and sperms were observed. The purification of active compounds present in Euphorbia aphylla and Ziziphus spina- christi plants may offers an alternative tool for the control of snails’ intermediate hosts of Schistosoma mansoni and Fasciola. Further investigations for Enterolobium contortisiliquum may increase its potential use as plant molluscicide are recommended