Improvement of medium components for high riboflavin
production by Aspergillus terreus using response surface
methodology

Research Abstract
The current study was designed to improve riboflavin production utilizing the filamentous fungus Aspergillus terreus. The effect of different nutritional and environmental factors was carried out by applying onefactor- at-a-time method. The selected high producer fungal strain was identified on the bases of the sequences of the gene encoding 18S rRNA and designated as A. terreus ASU1 (KP793450). In order to investigate the quantitative, optimal levels and the reciprocal interactions between five main medium components, response surface methodology (RSM) was subsequently employed. The results proved that optimal concentrations for enhancement of riboflavin production were: corn starch 30 (g/l); tryptone 0.5 (g/l) and 1 (g/l) for each glycine, KH2PO4 and MgSO4. The maximum riboflavin production of 344.60 mg/l (predicted 342.86 mg/l) was determined under the optimal conditions and the regression analysis (R2) of RSM showed 97.7 %. In comparison to the production of original level (63 mg/l), 5.4-fold increase had been obtained.
Research Authors
Nivien Allam Nafady Magdy Mohamed Khalil Bagy, Mohamed Hemida Abd-Alla,Fatthy Mohamed Morsy• Ghada Abd-Elmonsef Mahmoud
Research Department
Botany and Microbiology Department
Research Journal
Rendiconti Lincei
Research Member
Ghada Abd-Elmonsef Mahmoud
Research Pages
1-10
Research Publisher
Springer
Research Rank
1
Research Vol
DOI 10.1007/s12210-015-0449-7
Research Website
http://link.springer.com/article/10.1007/s12210-015-0449-7
Research Year
2015

Improvement of medium components for high riboflavin
production by Aspergillus terreus using response surface
methodology

Research Abstract
The current study was designed to improve riboflavin production utilizing the filamentous fungus Aspergillus terreus. The effect of different nutritional and environmental factors was carried out by applying onefactor- at-a-time method. The selected high producer fungal strain was identified on the bases of the sequences of the gene encoding 18S rRNA and designated as A. terreus ASU1 (KP793450). In order to investigate the quantitative, optimal levels and the reciprocal interactions between five main medium components, response surface methodology (RSM) was subsequently employed. The results proved that optimal concentrations for enhancement of riboflavin production were: corn starch 30 (g/l); tryptone 0.5 (g/l) and 1 (g/l) for each glycine, KH2PO4 and MgSO4. The maximum riboflavin production of 344.60 mg/l (predicted 342.86 mg/l) was determined under the optimal conditions and the regression analysis (R2) of RSM showed 97.7 %. In comparison to the production of original level (63 mg/l), 5.4-fold increase had been obtained.
Research Authors
Nivien Allam Nafady Magdy Mohamed Khalil Bagy, Mohamed Hemida Abd-Alla,Fatthy Mohamed Morsy• Ghada Abd-Elmonsef Mahmoud
Research Department
Botany and Microbiology Department
Research Journal
Rendiconti Lincei
Research Member
Mohamed Hemida Abd-Alla
Research Pages
1-10
Research Publisher
Springer
Research Rank
1
Research Vol
DOI 10.1007/s12210-015-0449-7
Research Website
http://link.springer.com/article/10.1007/s12210-015-0449-7
Research Year
2015

Improvement of medium components for high riboflavin
production by Aspergillus terreus using response surface
methodology

Research Abstract
The current study was designed to improve riboflavin production utilizing the filamentous fungus Aspergillus terreus. The effect of different nutritional and environmental factors was carried out by applying onefactor- at-a-time method. The selected high producer fungal strain was identified on the bases of the sequences of the gene encoding 18S rRNA and designated as A. terreus ASU1 (KP793450). In order to investigate the quantitative, optimal levels and the reciprocal interactions between five main medium components, response surface methodology (RSM) was subsequently employed. The results proved that optimal concentrations for enhancement of riboflavin production were: corn starch 30 (g/l); tryptone 0.5 (g/l) and 1 (g/l) for each glycine, KH2PO4 and MgSO4. The maximum riboflavin production of 344.60 mg/l (predicted 342.86 mg/l) was determined under the optimal conditions and the regression analysis (R2) of RSM showed 97.7 %. In comparison to the production of original level (63 mg/l), 5.4-fold increase had been obtained.
Research Authors
Nivien Allam Nafady Magdy Mohamed Khalil Bagy, Mohamed Hemida Abd-Alla,Fatthy Mohamed Morsy• Ghada Abd-Elmonsef Mahmoud
Research Department
Botany and Microbiology Department
Research Journal
Rendiconti Lincei
Research Member
Magdy Mohamed Khalil Bagy
Research Pages
1-10
Research Publisher
Springer
Research Rank
1
Research Vol
DOI 10.1007/s12210-015-0449-7
Research Website
http://link.springer.com/article/10.1007/s12210-015-0449-7
Research Year
2015

Mycoflora and mycotoxins of sunflower (Helianthus annuus L.) seeds in Egypt. I- Sugar fungi and natural occurrence of mycotoxins

Research Abstract
NULL
Research Authors
S.S. Mohamed El-Maraghy & O.M.O. El-Maghraby
Research Department
Botany and Microbiology Department
Research Journal
Qatar Univ. Bull. Sci.
Research Member
Saad Shehata Mohamed Ahmed El-Maraghy
Research Pages
107-121
Research Publisher
NULL
Research Rank
1
Research Vol
6
Research Website
NULL
Research Year
1986

Some biofertilziers relieved the stressful draw backs of calcareous soil upon seed (Nigella sativa L.) through inhibiting stress markers and antioxidant enzymes with enhancing plant growth

Research Abstract
NULL
Research Authors
Manal A. Zohri, Noha M, Full-Elnda M., S.S.M. El-Maraghy
Research Department
Botany and Microbiology Department
Research Journal
Egyptian J. Botany (In press).
Research Member
Saad Shehata Mohamed Ahmed El-Maraghy
Research Pages
NULL
Research Publisher
NULL
Research Rank
1
Research Vol
NULL
Research Website
NULL
Research Year
2016

A preliminary record of the entomopathogenic fungus Beauvaria bassiana (Balsamo) viullemin as endophyte in Egypt

Research Abstract
NULL
Research Authors
S.S. Mohamed El-Maraghy, M.A. Abdel-Rahman, K.A. Hussein and Shima A. Mohamed
Research Department
Botany and Microbiology Department
Research Journal
Pyrex J. Microbiology and Biotechnology Res.
Research Member
Saad Shehata Mohamed Ahmed El-Maraghy
Research Pages
9-14
Research Publisher
NULL
Research Rank
1
Research Vol
2 (2)
Research Website
NULL
Research Year
2016

Incidence of entomopathogenic fungi of the oat bird-cherry aphid, Rhopalosiphum padi L. (Homoptera: Phididae) infesting wheat plants in Assiut, Egypt

Research Abstract
NULL
Research Authors
S.M. El-Maraghy, Asmaa H. Mohamed, M.A. Abdel-Rahman, Azza A. Awad and Y.M. Omar
Research Department
Botany and Microbiology Department
Research Journal
J. Basic and Applied Mycology (Egypt)
Research Member
Saad Shehata Mohamed Ahmed El-Maraghy
Research Pages
43-47
Research Publisher
NULL
Research Rank
1
Research Vol
6
Research Website
NULL
Research Year
2015

Pathogenicity of Beauveria bassiana and Metarhizium anisopliae against Galleria mellanella

Research Abstract
NULL
Research Authors
Khalid, A. Hussein, M.A. Abdel-Rahman, A.Y. Abdel-Mallek, S.S. El-Maraghy & Jin Ho Joo
Research Department
Botany and Microbiology Department
Research Journal
Phytoparasitica, (In press).
Research Member
Saad Shehata Mohamed Ahmed El-Maraghy
Research Pages
NULL
Research Publisher
NULL
Research Rank
1
Research Vol
NULL
Research Website
NULL
Research Year
2010

Climatic factors interference with the occurrence of Beauveria bassiana and Metarhizium anisopliae in cultivated soil

Research Abstract
NULL
Research Authors
Khalid, A. Hussein, M.A. Abdel-Rahman, A.Y. Abdel-Mallek, S.S. El-Maraghy & Jin Ho Joo
Research Department
Botany and Microbiology Department
Research Journal
African J. Biotechnology,
Research Member
Saad Shehata Mohamed Ahmed El-Maraghy
Research Pages
7674-7682
Research Publisher
NULL
Research Rank
1
Research Vol
9 (45)
Research Website
NULL
Research Year
2010

Screening of fungi associated with grains and animal feeds in Al-Bayda Governorate, Libya

Research Abstract
NULL
Research Authors
Idres, H.A.; Laith, K.T.; Muftah, A.N.; Ibrahim, A.A.; Mazian, Z., El-Maraghy, S.S.M. and Razaul Karim, S.M.
Research Department
Botany and Microbiology Department
Research Journal
World Applied Sciences J.
Research Member
Saad Shehata Mohamed Ahmed El-Maraghy
Research Pages
746-756
Research Publisher
NULL
Research Rank
1
Research Vol
9 (7)
Research Website
NULL
Research Year
2010
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