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The effect of the electrical currents 3, 5 and 10 mA on glycogen, nonglycogen polysaccharides and total protein contents of hind limb regeneration in a premetamorphic stage (stage 56) and a metamorphic stage (stage 58) of the Egyptian toad, Bufo regularis Reuss was studied after amputation at the distal third of the thigh and mid shank levels. The amount of glycogen, mucopolysaccharides and total protein in the various tissues of limb regenerates was increased after 15 days of amputation that those of after five days both in control and experimental cases. It was suggested that the increase in polysaccharides and proteins are due to the restoration of aerobic cellular respiration and the relatively slow rate of building up new cells. Stimulation of the amputated limbs at the shank level was slightly more obvious than that at the thigh level.

The effect of the electrical currents 3, 5 and 10 mA on glycogen, nonglycogen polysaccharides and total protein contents of hind limb regeneration in a premetamorphic stage (stage 56) and a metamorphic stage (stage 58) of the Egyptian toad, Bufo regularis Reuss was studied after amputation at the distal third of the thigh and mid shank levels. The amount of glycogen, mucopolysaccharides and total protein in the various tissues of limb regenerates was increased after 15 days of amputation that those of after five days both in control and experimental cases. It was suggested that the increase in polysaccharides and proteins are due to the restoration of aerobic cellular respiration and the relatively slow rate of building up new cells. Stimulation of the amputated limbs at the shank level was slightly more obvious than that at the thigh level.

Glycogen and polysaccharides were found to be lesser after seven days of amputation than those after fifteen days due to the absence of aerobic cellular respiration in the former series.
Proteins also were found to be lesser in amount after seven days of amputation than those of fifteen days due to the high rate of cell proliferation in the former series

Glycogen and polysaccharides were found to be lesser after seven days of amputation than those after fifteen days due to the absence of aerobic cellular respiration in the former series.
Proteins also were found to be lesser in amount after seven days of amputation than those of fifteen days due to the high rate of cell proliferation in the former series

The fully developed oral disc of tadpoles of Bufo regularis consists of dorsal and ventral labia bearing, respectively, two and three ridges with numerous horny denticles having a dental formula , many lateral papillae on the dorsal and ventral labia that may have a sensory function, and a horny beak provided with jaw sheath serrations. At metamorphosis, these structures regress and disappear. Keratinization of the labial denticles and the beak serrations of the labial teeth occurs by the activity of the tonofilaments, while keratinization of the beak serration takes place by the cytoplasmic free ribosomes.

The fully developed oral disc of tadpoles of Bufo regularis consists of dorsal and ventral labia bearing, respectively, two and three ridges with numerous horny denticles having a dental formula , many lateral papillae on the dorsal and ventral labia that may have a sensory function, and a horny beak provided with jaw sheath serrations. At metamorphosis, these structures regress and disappear. Keratinization of the labial denticles and the beak serrations of the labial teeth occurs by the activity of the tonofilaments, while keratinization of the beak serration takes place by the cytoplasmic free ribosomes.