The present study aimed to investigate the hiso-morphological appearance and some biochemial changes of CL in relation to its functional activity during the estrous cycle and early pregnancy in buffalo- cows. The obtained results indicated the presence of two morphologically distinct steriogenic cells. There was a relationship between the average surface area of lueal component, the fine structure of luteal cells and serum progestron at different luteal phases and at early pregnancy. Changes in LPO, NO and SOD activity, played a significant role in regulation of CL function.

The present study aimed to investigate the hiso-morphological appearance and some biochemial changes of CL in relation to its functional activity during the estrous cycle and early pregnancy in buffalo- cows. The obtained results indicated the presence of two morphologically distinct steriogenic cells. There was a relationship between the average surface area of lueal component, the fine structure of luteal cells and serum progestron at different luteal phases and at early pregnancy. Changes in LPO, NO and SOD activity, played a significant role in regulation of CL function.

The tropical parasite Schistoma mansoni causes granulomatous inflammation after its eggs lodge in hepatic portal capillaries. The role of melatonin as antioxidants inprotection against oxidative stress in mice infected with S. mansoni was investigated. The results suggest that oxidative processes occur at the site of inflammation and are involved in the damaging effects of Schistomasiasis.Also, melatonin, due to its free radical scavenging activity, is highly protective against the pathological changes associated with Schistomasiasis.

The tropical parasite Schistoma mansoni causes granulomatous inflammation after its eggs lodge in hepatic portal capillaries. The role of melatonin as antioxidants inprotection against oxidative stress in mice infected with S. mansoni was investigated. The results suggest that oxidative processes occur at the site of inflammation and are involved in the damaging effects of Schistomasiasis.Also, melatonin, due to its free radical scavenging activity, is highly protective against the pathological changes associated with Schistomasiasis.

The stydy done on a total number of 48 cyclic corpora lutea were dissected from ovaries obtained from local abattoir and classified according to physico-anatomy to developing, fully developed and regressing. The study concluded that changes in LPO and NO production and antioxidant activities is a dynamic and regulated process, which could play an important role in regulating luteal function during esterous cycle in buffalo cows.

The stydy done on a total number of 48 cyclic corpora lutea were dissected from ovaries obtained from local abattoir and classified according to physico-anatomy to developing, fully developed and regressing. The study concluded that changes in LPO and NO production and antioxidant activities is a dynamic and regulated process, which could play an important role in regulating luteal function during esterous cycle in buffalo cows.

The results of differential scanning calorimetry (DSC) under non-isothermal conditions of the chalco-genide Inx(Se0.75Te0.25)100-x(where 0≤x≤10 at.%) glasses are reported and discussed. The dependence of the characteristic temperatures "Glass transition temperature (Tg), the crystallization onset temperature (Tc) and the crystallization peak temperature (Tp) on the heating rate (α) utilized in the determination of the activation (Eg), the activation energy for crystallization (Ec) and the Avrami,s exponent (n). The composition dependence of the Tg , Eg , and Ec were discussed in terms of the chemical bond approach, the average heats of atomization (Hs) and the cohesive energy (CE). The diffractogram of the transformed of the transformed material shows the presence of some crystallites of Se-Te and In-Se in the residual amorphous matrix.

A fraction with bradykinin potentinating activity was chemically isolated from the venom of the Egyptian scorpion Buthus occitanus. The pharamacological activity of the fraction (BPF) was bioassayed by the ability to potentiate added BK on the isolated guinea pig ileum as well as its inhibitory activity on ACE. The mode of action of this fraction on gonads of immature female guinea pigs was measured. The fraction was i.p. injected 1 ug/g body weight for five times in animalsat successive time intervals of 7 days each. Total body, ovaries, and uterine tissue weights were significantly increased as well as uterine total protein, RNA and cyclic nucleotides contents. Concomitantly, PGE2 level showed a significant elevation in both uterine and ovarian tissues. The uterine tissue homogenates in vitro showed an enhancing effect in response to the added fraction (1 ug/ml) and BK (i ug/ml) for PG biosynthesis from radiolabelled precursor 14 C-linoleic acid into its labelled metabolites AA, PGD2, TxB2, PGE1, PGF1 a and 6-keyo PGF1a.This enhancement effect was abolished in the presence of BK inhibitor , but the labelled PGF2a was still high. The results clearly indicate that the increase of PGs resulting from both in vivo & in vitro experiment may contribute to the inhibition of ACE and potentiation of exogenous and endognous BK.

A fraction with bradykinin potentinating activity was chemically isolated from the venom of the Egyptian scorpion Buthus occitanus. The pharamacological activity of the fraction (BPF) was bioassayed by the ability to potentiate added BK on the isolated guinea pig ileum as well as its inhibitory activity on ACE. The mode of action of this fraction on gonads of immature female guinea pigs was measured. The fraction was i.p. injected 1 ug/g body weight for five times in animalsat successive time intervals of 7 days each. Total body, ovaries, and uterine tissue weights were significantly increased as well as uterine total protein, RNA and cyclic nucleotides contents. Concomitantly, PGE2 level showed a significant elevation in both uterine and ovarian tissues. The uterine tissue homogenates in vitro showed an enhancing effect in response to the added fraction (1 ug/ml) and BK (i ug/ml) for PG biosynthesis from radiolabelled precursor 14 C-linoleic acid into its labelled metabolites AA, PGD2, TxB2, PGE1, PGF1 a and 6-keyo PGF1a.This enhancement effect was abolished in the presence of BK inhibitor , but the labelled PGF2a was still high. The results clearly indicate that the increase of PGs resulting from both in vivo & in vitro experiment may contribute to the inhibition of ACE and potentiation of exogenous and endognous BK.

Transferrin receptor mediates internalization of transferrin with bound ferric ions through the clathrindependent
pathway. We found that binding of transferrin to the receptor induced rapid generation of cell
surface ceramide which correlated with activation of acid, but not neutral, sphingomyelinase. At the onset
of transferrin internalization both ceramide level and acid sphingomyelinase activity returned to their
basic levels. Down-regulation of acid sphingomyelinase in cells with imipramine or silencing of the enzyme
expression with siRNA stimulated transferrin internalization and inhibited its recycling. In these
conditions colocalization of transferrin with clathrin was markedly reduced. Simultaneously, K+ depletion
of cells which interfered with the assembly of clathrin-coated pits inhibited the uptake of transferrin
much less efficiently than it did in control conditions. The down-regulation of acid sphingomyelinase activity
led to the translocation of transferrin receptor to the raft fraction of the plasma membrane upon
transferrin binding. The data suggest that lack of cell surface ceramide, generated in physiological conditions
by acid sphingomyelinase during transferrin binding, enables internalization of transferrin/transferrin
receptor complex by clathrin-independent pathway.