The present work rationalizes the construction of a cutting-edge electrochemical sensor and its utilization in establishing the unique square wave voltammetric (SWV) procedure for the concurrent analysis of a ternary mixture of anti-Parkinson drugs (L-dopa (LVD), Carbidopa (CRD), and Istradefylline (ISF)) in bulk forms and plasma specimens. The sensor, denoted as TbNPs@poly-PRS/PGE, was designed which based on terbium nanoparticles (TbNPs) decorated Pyrosin (PRS) stain polymer platform on the surface of pencil graphite electrode (PGE). The improved electrochemical efficiency and morphological features of the sensor was assured through various investigations including cyclic voltammetry, scanning electron microscopy, electrochemical impedance spectroscopy, and theoretical computational modelling. This sensor was devoted to electrooxidization and simultaneous quantitation of LVD-CRD-ISF …

Based on a previous study from our group, fifteen new tetrahydrocarbazoles (THC) incorporating 5-arylidene-4-thiazolinone scaffolds at one position were synthesized and characterized by spectroscopic, elemental and X-ray crystallographic techniques. The new hybrids were evaluated for their anti-proliferative activity against a panel of eight human cancer cell lines utilizing Sulforhodamine B (SRB) assay with doxorubicin as a reference. Among all synthesized THC based hybrids, compounds 11c and 12c revealed the highest potency and selectivity against Jurkat (IC₅₀ 1.44 ± 0.09 μM), U937 (IC₅₀ 1.77 ± 0.08 μM) and HCT-116 (IC₅₀ 6.75 ± 0.08 μM) cell lines, while against normal cell line HME1 (IC₅₀ > 50 μM). To validate the multitarget approach, topoisomerases, tubulin and EGFR inhibition assays were performed. Results indicate that compound 11c inhibited Topo Iα (IC₅₀ 52.12 μM), Topo IIα …

Two series of benzofuran-based thiosemicarbazides 7, 8(a-l) are evaluated as P. aeruginosa quorum sensing inhibitors (QSIs). The studied compounds resulted in reduction of violacein production of C. violaceum, at ¼ MIC, up to 26.04 – 67.36%, relative to the reference drug azithromycin (54%). In vitro evaluation of QS and antivirulence activities, at the same concentration, against P. aeruginosa, showed promising inhibitory effect of biofilms formation, bacterial motility and virulence factors production (pyocyanin, and rhamnolipids). Compounds 7a, 7d, and 7k inhibit biofilm formation up to 57.31%, 67.54%, 78.38%, respectively, in addition to > 50% inhibition of hemolysin production. On the other hand, compounds 8a, 8d, 8f and 8k of the bromobenzofuran series showed, 55.66%, 50.67%, 73.34%, 79.93%, reduction in biofilm formation, respectively. Compounds 8d and 8f exhibit > 50% reduction of hemolysin

Accumulating evidence has indicated that immune regulatory cells are involved in the establishment of the anti-tumor activity, however; the role of regulatory B cells (B-regs) in breast cancer (BC) remains unclear. This study intended to assess the frequency of peripheral B-regs phenotypes in patients with BC, and to determine the relation between these phenotypes and the patient's clinicopathological characters. The expressions of the immune cell populations were analyzed by four-color flow cytometry in 40 naïve BC patients and 10 age-matched apparently healthy individuals as controls attending the department of Clinical Oncology and Nuclear Medicine at Assiut University Hospitals. The percentages of B-regs phenotypes CD19+IL10+ and CD19+CD24hiCD27+IL10+ were higher in BC patients than in the controls. The percentage of CD19+IL10+ B cells phenotype was significantly associated with the HER-2 expression levels, T, and N stages of BC. In conclusion, high percentage of B-regs phenotypes CD19+IL10+ and CD19+CD24hiCD27+IL10+ in BC patients indicates a possible role in immune suppression during the development of BC.