Dual-emissive gold and silver nanoclusters stabilized by bovine serum albumin (BSA@Au-Ag NCs) were engineered for the precise and sensitive estimation of acrylamide (ACM). The probe demonstrated emissions at 630 nm and 405 nm upon excitation at 320 nm. Decreasing the fluorescence of the probe at 630 nm was observed upon cysteine (Cys) addition while the emission band at 405 nm experienced a slight increase. The introduction of ACM into the probe led to the recovery of fluorescence at 630 nm, while the fluorescence at 405 nm remained largely unaffected. This selective response provides a ratiometric method for determining ACM. A linear relationship between the response ratio (F₆₃₀/F₄₀₅) and ACM concentration was established in the range of 0.12–450 µM, with LOD of 0.03 µM. The fluorescent probe boasts several advantages, including a low LOD, robustness against interference, and …

For the first time, we introduce a new disposable and ultrasensitive sensing electrode made up of nanostructured gold anchored porous carbon black (Au@PCB) sol–gel nanoceramic conductive film (CF) coated eco-friendly and inexpensive pencil graphite rods (PGRs) to explore their electro-catalytic sensing of dopamine (DA) in the absence and presence of uric acid (UA). X-ray diffraction (XRD) and scanning electron microscopy combined with energy-dispersive X-ray spectroscopy (SEM-EDX) were used for evaluating the Au@PCB nanocomposite. The CV analysis revealed that the disposable Au@PCB-CF/PGRE sensor exhibited outstanding redox activities in the optimal probe [Fe(CN)₆]^3−/4−. The conductive Au@PCB nanocomposite exhibits exceptional electrochemical characteristics, a larger electrochemically active surface area, and remarkable electro-catalytic activity. As a result, the oxidation current of

A novel fluorometric method for the determination of L-asparaginase, an enzyme crucial in cancer therapy and food industry applications, is presented. This sensitive and selective approach utilizes L-asparagine and two pH-sensitive carbon dots (blue-N-CDs and red-N-CDs) as probes. The interaction between L-asparagine and L-asparaginase liberates ammonia, causing an increase in pH. This pH change simultaneously decreases the fluorescence of blue-N-CDs while enhancing the emission of red-N-CDs, enabling ratiometric detection of L-asparaginase. Comprehensive characterization of both carbon dots and investigation of their response mechanism towards L-asparaginase were conducted using ultraviolet–visible spectrophotometry, fluorescence spectroscopy, and transmission electron microscopy (TEM) imaging techniques. The designed approach demonstrates outstanding linearity (20 to 2000 U L^-1 …

A new fluorescence sensing approach has been proposed for the precise determination of the anti-cancer drug oxaliplatin (Oxal-Pt). This method entails synthesizing blue-emitting copper nanoclusters (CuNCs) functionalized with bovine serum albumin (BSA) as the stabilizing agent. Upon excitation at 360 nm, the resultant probe exhibits emission at 460 nm. Notably, the fluorescence response of BSA@CuNCs substantially increases upon incubation with Oxal-Pt due to multiple binding interactions between the drug and the fluorescent probe. These interactions involve hydrogen bonding, hydrophobic interaction, and the high affinity between the SH groups (cysteine residues of BSA) and platinum (in Oxal-Pt). Consequently, this interaction induces aggregation-induced emission enhancement (AIEE) of BSA@CuNCs. The probe demonstrates a broad response range from 0.08 to 140.0 μM, along with a low detection