Root-rot of cotton (Gossypium spp.) is one of the most important diseases in Upper Egypt. Isolation has been done from diseased cotton roots and seeds which were collected from 11 counties in Assiut province, Egypt. Identification procedures of the isolated fungi confirmed that the isolated fungi were for Fusarium solani, F. moniliforme and F. oxysporum and Rhizoctonia solani. Thirty six isolates of Fusarium spp. and 10 isolates of Rhizoctonia solani were tested for their pathogenicity on both Giza 80 and Giza 83 cotton seedlings to verify their virulence on seedlings. The pathogenicity test results have grouped the Fusarium spp. isolates into three groups; highly virulent that caused 91-100% mortalities; moderately virulent that caused 81-90% mortalities and low virulent that caused lower than 81.0% mortalities. Data also shows that, in general Giza 80 cotton cultivar was more susceptible for infection with Fusarium spp. when compared with Giza 83 cotton cultivar. In case of Rhizoctonia solani, data revealed that the infection percentage was significantly affected by isolates while cotton cultivars had no significant influence on infection. Four 10-mer primers (1:6-d, 2:6-d, 4:6-d and 5:6-d) were used in RAPD-PCR to determine the genetic variability between six isolates, one virulent and one less virulent, of F. moniliforme, F. oxysporum and F. solani. Our results showed that the primer 2:6-d clearly separated F. moniliforme, F. oxysporum and F. solani and proved to be quite powerful in distinguishing the three different species and isolates of Fusarium spp.

Root-rot of cotton (Gossypium spp.) is one of the most important diseases in Upper Egypt. Isolation has been done from diseased cotton roots and seeds which were collected from 11 counties in Assiut province, Egypt. Identification procedures of the isolated fungi confirmed that the isolated fungi were for Fusarium solani, F. moniliforme and F. oxysporum and Rhizoctonia solani. Thirty six isolates of Fusarium spp. and 10 isolates of Rhizoctonia solani were tested for their pathogenicity on both Giza 80 and Giza 83 cotton seedlings to verify their virulence on seedlings. The pathogenicity test results have grouped the Fusarium spp. isolates into three groups; highly virulent that caused 91-100% mortalities; moderately virulent that caused 81-90% mortalities and low virulent that caused lower than 81.0% mortalities. Data also shows that, in general Giza 80 cotton cultivar was more susceptible for infection with Fusarium spp. when compared with Giza 83 cotton cultivar. In case of Rhizoctonia solani, data revealed that the infection percentage was significantly affected by isolates while cotton cultivars had no significant influence on infection. Four 10-mer primers (1:6-d, 2:6-d, 4:6-d and 5:6-d) were used in RAPD-PCR to determine the genetic variability between six isolates, one virulent and one less virulent, of F. moniliforme, F. oxysporum and F. solani. Our results showed that the primer 2:6-d clearly separated F. moniliforme, F. oxysporum and F. solani and proved to be quite powerful in distinguishing the three different species and isolates of Fusarium spp.

Root-rot of cotton (Gossypium spp.) is one of the most important diseases in Upper Egypt. Isolation has been done from diseased cotton roots and seeds which were collected from 11 counties in Assiut province, Egypt. Identification procedures of the isolated fungi confirmed that the isolated fungi were for Fusarium solani, F. moniliforme and F. oxysporum and Rhizoctonia solani. Thirty six isolates of Fusarium spp. and 10 isolates of Rhizoctonia solani were tested for their pathogenicity on both Giza 80 and Giza 83 cotton seedlings to verify their virulence on seedlings. The pathogenicity test results have grouped the Fusarium spp. isolates into three groups; highly virulent that caused 91-100% mortalities; moderately virulent that caused 81-90% mortalities and low virulent that caused lower than 81.0% mortalities. Data also shows that, in general Giza 80 cotton cultivar was more susceptible for infection with Fusarium spp. when compared with Giza 83 cotton cultivar. In case of Rhizoctonia solani, data revealed that the infection percentage was significantly affected by isolates while cotton cultivars had no significant influence on infection. Four 10-mer primers (1:6-d, 2:6-d, 4:6-d and 5:6-d) were used in RAPD-PCR to determine the genetic variability between six isolates, one virulent and one less virulent, of F. moniliforme, F. oxysporum and F. solani. Our results showed that the primer 2:6-d clearly separated F. moniliforme, F. oxysporum and F. solani and proved to be quite powerful in distinguishing the three different species and isolates of Fusarium spp.

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A field experiment was carried out during 2017/18 and 2018/19 seasons at the Agronomy Department Experimental Farm, Agriculture Faculty, Assiut University, to study the effect of foliar spray by some Nano micro-nutrients on production of three bread wheat cultivars. The experiment was laid out in randomized complete block design (RCBD) using strip plot arrangement with three replications. Foliar spray with tap water (solvent as a control) and Foliar spray with Fe, Mn, Zn, Fe+Mn, Fe+Zn, Mn+Zn and Fe+Mn+Zn in Nano form at 200 ppm were allocated horizontally, while bread wheat cultivars (Sids-1, Sids-12 and Gemmeaza-11) were arranged vertically. The obtained results show that the foliar spray treatment by some micro-nutrients Nano-particles had a significant effect on plant height, kernels number spike-1 , 1000 kernel weight, kernel weight spike-1 and grain yield in both seasons. Thus, wheat plants which were sprayed by Fe+Mn+Zn produced the highest mean values of the most previous traits. Furthermore the studied cultivars had a significant effect on all studied traits. In addition, Gemmeaza-11 cultivar produced the highest mean values of most studied traits in the two growing seasons. Here too, the interaction between some micro-nutrients Nano-particles and bread wheat cultivars had a highly significant (P ≤ 0.01) and significant (P ≤ 0.05) influence on grain yield in the first and second seasons respectively. Thus, the highest mean values of grain yield (26.80 and 26.95 ardab fed.-1 in the tow respective seasons) were obtained from Gemmeaza11 cultivar which was sprayed by Fe+Mn+Zn Nano-particles in both seasons.

A field experiment was carried out during 2017/18 and 2018/19 seasons at the Agronomy Department Experimental Farm, Agriculture Faculty, Assiut University, to study the effect of foliar spray by some Nano micro-nutrients on production of three bread wheat cultivars. The experiment was laid out in randomized complete block design (RCBD) using strip plot arrangement with three replications. Foliar spray with tap water (solvent as a control) and Foliar spray with Fe, Mn, Zn, Fe+Mn, Fe+Zn, Mn+Zn and Fe+Mn+Zn in Nano form at 200 ppm were allocated horizontally, while bread wheat cultivars (Sids-1, Sids-12 and Gemmeaza-11) were arranged vertically. The obtained results show that the foliar spray treatment by some micro-nutrients Nano-particles had a significant effect on plant height, kernels number spike-1 , 1000 kernel weight, kernel weight spike-1 and grain yield in both seasons. Thus, wheat plants which were sprayed by Fe+Mn+Zn produced the highest mean values of the most previous traits. Furthermore the studied cultivars had a significant effect on all studied traits. In addition, Gemmeaza-11 cultivar produced the highest mean values of most studied traits in the two growing seasons. Here too, the interaction between some micro-nutrients Nano-particles and bread wheat cultivars had a highly significant (P ≤ 0.01) and significant (P ≤ 0.05) influence on grain yield in the first and second seasons respectively. Thus, the highest mean values of grain yield (26.80 and 26.95 ardab fed.-1 in the tow respective seasons) were obtained from Gemmeaza11 cultivar which was sprayed by Fe+Mn+Zn Nano-particles in both seasons.