Various biomaterials have been evaluated to enhance bone formation in critical-sized bone defects; however, the ideal scaffold is still missing. The objective of this study was to investigate the in vitro and in vivo regenerative capacity of graphitic carbon nitride (g-C₃N₄) and graphene oxide (GO) nanomaterials to stimulate critical-sized bone defect regeneration. The in vitro cytotoxicity and hemocompatibility of g-C₃N₄ and GO were evaluated, and their potential to induce the in vitro osteogenesis of human fetal osteoblast (hFOB) cells was assessed using qPCR. Then, bone defect in femoral condyles was created in rabbits and left empty as control or filled with either g-C₃N₄ or GO. The osteogenesis of the different implanted scaffolds was evaluated after 4, 8, and 12 weeks of surgery using X-ray, computed tomography (CT), macro/microscopic examinations, and qPCR analysis of osteocalcin (OC) and osteopontin (OP) expressions. Both materials displayed good cell viability and hemocompatibility with enhanced collagen type-I (Col-I), OC, and OP expressions of the hFOB cells. Compared to the control group, the bone healing process in g-C₃N₄ and GO groups was promoted in vivo. Moreover, complete healing of the bone defect was observed radiologically and grossly in g-C₃N₄ implanted group. Additionally, g-C3N4 implanted group showed higher percentages of osteoid tissue, mature collagen, biodegradation, and expressions of OC and OP. In conclusion, our results revealed that g-C₃N₄ and GO nanomaterials could induce osteogenesis in critical-sized bone defects.

Several volcanic buildups have been documented using gravity and magnetic data at specific locations in the Northern Red Sea (NRS). Most of these volcanoes were never sampled, and only a few were imaged by seismic or bathymetry data. Furthermore, the confidentiality of commercial datasets does not allow adequate knowledge of these structures and their morphology and physical properties. In this study, 2D and 3D seismic reflection data and 3D constrained inversion of magnetic and gravity data have been used to infer densities and magnetic susceptibilities of volcanic features in the central part of the NRS and to evaluate the distribution and geometry. This study indicates that the results of the 3D inverted models show a good fit with the volcanic structures inferred from seismic data and, thus, that the inversion technique adopted can be a reliable tool for detecting other edifices where seismic data are …

The Red Sea basin includes a thick Middle to Late Miocene evaporitic succession that underwent halokinesis and caused intensive reshaping of the seafloor and the development of salt-tectonic structures. However, the geometry and kinematics of these structures are still poorly understood. This study uses 2D and 3D seismic surveys and well data of the northern Egyptian Red Sea to systematically describe the distribution and morphology of salt structures, discuss their initiation, and construct a kinematic model for their origin. Our results indicate that the massive salt layer developed into five major NW-SE to NNE-SSW trending salt walls, characterized by relatively irregular crests and moderately dipping flanks. In addition, several symmetrical and asymmetrical folds and two categories of normal faults (subsalt and suprasalt) have been recognized. Based on our observations, salt mobilization in the study area …

The adverse impact of schistosomiasis on tissues is considered in generating a schistosomal vaccine. The purpose of this study was to evaluate the effectiveness of Schistosoma mansoni crude antigens as a therapeutic and prophylactic formulation in the inhibition of heat shock protein, apoptosis, and CD3/CD20 expression in a liver and spleen mouse models using the immunohistochemistry method. A total of 65 mice were divided into five groups: (i) infected untreated group (G1), (ii) therapeutic treated group (G2) with egg soluble egg antigen (SEA), and soluble worm antigen preparation (SWAP), (iii) prophylactically treated group (G3) with cercarial antigen preparation (CAP), (iv) combined treated group with three antigens (G4), and (v) control group (G5). The results we obtained showed that CAP, SEA, and SWAP antigens mitigated the deterioration and inflammation induced by infection. Apoptosis and …

Global crop yield and growth are severely impacted by hazardous heavy metal contamination of the soil and water. This study examined the effects on a range of physiological, biochemical, and morphological parameters of two canola (Brassica napus L.) cultivars, Punjab (V1) and Super (V2), when they were subjected to three different levels of cadmium (Cd) stress (0, 1.5, and 2.25 mM) and foliar application of biostimulant, ie, Moringa leaf extract (MLE). Plants in V1 (Punjab) collect more Cd in both their aerial and subterranean sections than in V2 (Super). The morphological and physiological indicators showed a substantial (p< 0.05) deterioration as a result of the Cd buildup. Ascorbic acid and malondialdehyde (MDA) and hydrogen peroxide (H2O2) were considerably (p< 0.05) elevated under Cd stress. V1 has considerably higher H2O2 values than Super (V2), which suggests that oxidative stress levels there are higher. Additionally, the activity of some antioxidant enzymes, such as catalase and peroxidase (65 and 40%, respectively), were reduced by a higher concentration of Cd (2.25 mM). V2 demonstrated stronger antioxidant defence mechanisms than V1, as seen by a 115% rise in POD activity, a 70% increase in shoot fresh weight, and a 49% increase in amino acids following MLE spray. Despite this, the V2 was still able to withstand Cd. Under Cd stress, foliar treatment of MLE increased both kinds' yield, growth, biochemical, and physiological characteristics. The findings indicated that applying MLE foliar spray can improve crop productivity and lessen the impacts of metals on B. napus L.