The final carbonation mud resulted from sugar beet clarification process has been and still represents the most common waste problem in sugar industry. It contains mainly calcium carbonate beside other minor constituents. In this study, a novel technique was developed, which involves combination of Limex method and sol–gel method for the preparation of calcium oxide nanoparticles (CON) from the carbonation mud waste. The synthesized nanoparticles were characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), Fourier-transform infrared spectroscopy and X-ray diffraction. The SEM and TEM images showed that the CON particles are semi-hexagonal in morphology with particles size 60–275 nm. A high surface area 747.62 m²/g was also determined. Minerals analysis of CON showed high content of calcium oxide (92.81%) and significant reduction in the percentage of …

Men with non-obstructive azoospermia constitute a challenging subgroup of male infertility patients in whom a genetic cause of defective spermatogenesis may be a contributing factor. The aim of this prospective observational cohort study was to determine whether assessment of meiotic nuclear division 1 (MND1) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene expression (MND1/GAPDH) in testicular tissue could be a prognostic indicator for sperm retrieval and ICSI outcome in patients with non-obstructive azoospermia. The study participants underwent clinical evaluation, conventional semen analysis, serum follicular stimulating hormone (FSH), testosterone assay, scrotal ultrasound examination, microsurgical testicular sperm extraction (mTESE), and assessment of MND1/GAPDH gene expression levels in testicular tissue via quantitative polymerase chain reaction (qPCR) techniques

Few studies investigated yeasts potentiality as plant growth promoters as well as the yeasts ability to mitigate unfavorable conditions for plants by improving their compliance with stress. In this study, we tested eight yeast strains (Yarrowia lipolytica YEAST-1, Candida diddensiae YEAST-2, Trichosporon gamsii YEAST-5, T. ovoides YEAST-6, Y. lipolytica YEAST-16, C. subhashii YEAST-17, Saccharomyces cerevisiae YEAST-30, and S. cerevisiae YEAST-34) involving plant growth-promotion (PGP) traits to alleviate salinity stress on the early seed germination of Triticum aestivum L. Also, their ability to form biofilm was detected. Y. lipolytica YEAST-1 yeast strain enhanced the plumule length of T. aestivum seedling by more than 4.0, 3.0, and 2.0 cm at salinity stress of 50, 100, and 200 mM NaCl, respectively, after 96 h. The genes required for the synthesis of the extracellular amino-cyclopropane-1-carboxylate deaminase (ACCD) displayed the highest expression level in S. cerevisiae YEAST-34, at 5 mM ACC. S. cerevisiae YEAST-34, Y. lipolytica YEAST-1, and C. subhashii YEAST-17 exhibited a significant (P < 0.05) increase in the ACCD gene expression by adding different levels of ACC. Y. lipolytica YEAST-1 inoculation enhanced the radicle length of T. aestivum seedling significantly by 2.0 cm at 0.0 mM NaCl, 0.8 cm at 50 mM NaCl, 0.7 at 100 mM NaCl, and 0.06 cm at 200 mM NaCl stress. This improvement of plant growth under salinity stress may be due to the yeast's ability to secrete the extracellular amino-cyclopropane-1-carboxylate deaminase.