Abstract
The present study aims to investigate the variation
in vegetation and species diversity in three
inland wadis (Wadi Solaf, W. Romana and W. El-
Akhdar) which drain their water from surrounding
high mountains to the main channel
of Wadi Feiran in South Sinai. It attempted to
compare the floristic diversity between these
wadis to recognize the different distribution
patterns of species, and to assess the role of the
edaphic factors which control the distribution
of the plant communities. Forty-five sample
plots were selected to represent as much as possible
the variation in the vegetation, and georeferenced
using GPS techniques. A total of 116
species (45 annuals and 71 perennials) belonging
to 95 genera and 37 families were recorded,
with Asteraceae, Brassicaceae, Fabaceae, and
Zygophyllaceae represented the species-rich
families. Therophytes constituted the main bulk
of the flora, followed by chamaephytes,
phanerophytes and hemicryptophytes. As part
of the Saharo-Arabian region, the Saharo-Arabian
chorotype dominated the others. Classification
of the vegetation resulted in 5 vegetation
groups: (A) Zilla spinosa, (B) Artemisia
judaica-Zilla spinosa, (C) Artemisia judaica, (D)
Anabasis articulata-Artemisia judaica-Fagonia
mollis, and (E) Fagonia mollis-Zilla spinosa.
Species richness was significantly negatively correlated
with chlorides, while the Shannon’s
diversity index showed significant negative correlation
with chlorides and positively correlated
with calcium. Vegetation-soil relationships were
assessed by Canonical Correspondence Analysis
(CCA) using 13 soil factors indicated that gravel,
coarse sand, fine sand, silt, clay, sodium and
chlorides were the key soil variables that affect
the distribution of plant communities in the
inland wadis of South Sinai.

Egyptian soils are generally characterized by slightly alkaline to alkaline pH values (7.5–8.7) which are
mainly due to its dry environment. In arid and semi-arid regions, salts are less concentrated and sodium
dominates in carbonate and bicarbonate forms, which enhance the formation of alkaline soils. Alkaline
soils have fertility problems due to poor physical properties which adversely affect the growth and the
yield of crops. Therefore, this study was devoted to investigating the synergistic interaction of Rhizobium
and arbuscular mycorrhizal fungi for improving growth of faba bean grown in alkaline soil. A total of
20 rhizobial isolates and 4 species of arbuscular mycorrhizal fungi (AMF) were isolated. The rhizobial
isolates were investigated for their ability to grow under alkaline stress. Out of 20 isolates 3 isolates were
selected as tolerant isolates. These 3 rhizobial isolates were identified on the bases of the sequences of
the gene encoding 16S rRNA and designated as Rhizobium sp. Egypt 16 (HM622137), Rhizobium sp. Egypt
27 (HM622138) and Rhizobium leguminosarum bv. viciae STDF-Egypt 19 (HM587713). The best alkaline
tolerant was R. leguminosarum bv. viciae STDF-Egypt 19 (HM587713). The effect of R. leguminosarum
bv. viciae STDF-Egypt 19 and mixture of AMF (Acaulospora laevis, Glomus geosporum, Glomus mosseae
and Scutellospora armeniaca) both individually and in combination on nodulation, nitrogen fixation and
growth of Vicia faba under alkalinity stress were assessed. A significant increase over control in number
and mass of nodules, nitrogenase activity, leghaemoglobin content of nodule, mycorrhizal colonization,
dry mass of root and shoot was recorded in dual inoculated plants than plants with individual inoculation.
The enhancement of nitrogen fixation of faba bean could be attributed to AMF facilitating the
mobilization of certain elements such as P, Fe, K and other minerals that involve in synthesis of nitrogenase
and leghaemoglobin. Thus it is clear that the dual inoculation with Rhizobium and AMF biofertilizer
is more effective for promoting growth of faba bean grown in alkaline soils than the individual treatment,
reflecting the existence of synergistic relationships among the inoculants.

Egyptian soils are generally characterized by slightly alkaline to alkaline pH values (7.5–8.7) which are
mainly due to its dry environment. In arid and semi-arid regions, salts are less concentrated and sodium
dominates in carbonate and bicarbonate forms, which enhance the formation of alkaline soils. Alkaline
soils have fertility problems due to poor physical properties which adversely affect the growth and the
yield of crops. Therefore, this study was devoted to investigating the synergistic interaction of Rhizobium
and arbuscular mycorrhizal fungi for improving growth of faba bean grown in alkaline soil. A total of
20 rhizobial isolates and 4 species of arbuscular mycorrhizal fungi (AMF) were isolated. The rhizobial
isolates were investigated for their ability to grow under alkaline stress. Out of 20 isolates 3 isolates were
selected as tolerant isolates. These 3 rhizobial isolates were identified on the bases of the sequences of
the gene encoding 16S rRNA and designated as Rhizobium sp. Egypt 16 (HM622137), Rhizobium sp. Egypt
27 (HM622138) and Rhizobium leguminosarum bv. viciae STDF-Egypt 19 (HM587713). The best alkaline
tolerant was R. leguminosarum bv. viciae STDF-Egypt 19 (HM587713). The effect of R. leguminosarum
bv. viciae STDF-Egypt 19 and mixture of AMF (Acaulospora laevis, Glomus geosporum, Glomus mosseae
and Scutellospora armeniaca) both individually and in combination on nodulation, nitrogen fixation and
growth of Vicia faba under alkalinity stress were assessed. A significant increase over control in number
and mass of nodules, nitrogenase activity, leghaemoglobin content of nodule, mycorrhizal colonization,
dry mass of root and shoot was recorded in dual inoculated plants than plants with individual inoculation.
The enhancement of nitrogen fixation of faba bean could be attributed to AMF facilitating the
mobilization of certain elements such as P, Fe, K and other minerals that involve in synthesis of nitrogenase
and leghaemoglobin. Thus it is clear that the dual inoculation with Rhizobium and AMF biofertilizer
is more effective for promoting growth of faba bean grown in alkaline soils than the individual treatment,
reflecting the existence of synergistic relationships among the inoculants.

Egyptian soils are generally characterized by slightly alkaline to alkaline pH values (7.5–8.7) which are
mainly due to its dry environment. In arid and semi-arid regions, salts are less concentrated and sodium
dominates in carbonate and bicarbonate forms, which enhance the formation of alkaline soils. Alkaline
soils have fertility problems due to poor physical properties which adversely affect the growth and the
yield of crops. Therefore, this study was devoted to investigating the synergistic interaction of Rhizobium
and arbuscular mycorrhizal fungi for improving growth of faba bean grown in alkaline soil. A total of
20 rhizobial isolates and 4 species of arbuscular mycorrhizal fungi (AMF) were isolated. The rhizobial
isolates were investigated for their ability to grow under alkaline stress. Out of 20 isolates 3 isolates were
selected as tolerant isolates. These 3 rhizobial isolates were identified on the bases of the sequences of
the gene encoding 16S rRNA and designated as Rhizobium sp. Egypt 16 (HM622137), Rhizobium sp. Egypt
27 (HM622138) and Rhizobium leguminosarum bv. viciae STDF-Egypt 19 (HM587713). The best alkaline
tolerant was R. leguminosarum bv. viciae STDF-Egypt 19 (HM587713). The effect of R. leguminosarum
bv. viciae STDF-Egypt 19 and mixture of AMF (Acaulospora laevis, Glomus geosporum, Glomus mosseae
and Scutellospora armeniaca) both individually and in combination on nodulation, nitrogen fixation and
growth of Vicia faba under alkalinity stress were assessed. A significant increase over control in number
and mass of nodules, nitrogenase activity, leghaemoglobin content of nodule, mycorrhizal colonization,
dry mass of root and shoot was recorded in dual inoculated plants than plants with individual inoculation.
The enhancement of nitrogen fixation of faba bean could be attributed to AMF facilitating the
mobilization of certain elements such as P, Fe, K and other minerals that involve in synthesis of nitrogenase
and leghaemoglobin. Thus it is clear that the dual inoculation with Rhizobium and AMF biofertilizer
is more effective for promoting growth of faba bean grown in alkaline soils than the individual treatment,
reflecting the existence of synergistic relationships among the inoculants.

Egyptian soils are generally characterized by slightly alkaline to alkaline pH values (7.5–8.7) which are
mainly due to its dry environment. In arid and semi-arid regions, salts are less concentrated and sodium
dominates in carbonate and bicarbonate forms, which enhance the formation of alkaline soils. Alkaline
soils have fertility problems due to poor physical properties which adversely affect the growth and the
yield of crops. Therefore, this study was devoted to investigating the synergistic interaction of Rhizobium
and arbuscular mycorrhizal fungi for improving growth of faba bean grown in alkaline soil. A total of
20 rhizobial isolates and 4 species of arbuscular mycorrhizal fungi (AMF) were isolated. The rhizobial
isolates were investigated for their ability to grow under alkaline stress. Out of 20 isolates 3 isolates were
selected as tolerant isolates. These 3 rhizobial isolates were identified on the bases of the sequences of
the gene encoding 16S rRNA and designated as Rhizobium sp. Egypt 16 (HM622137), Rhizobium sp. Egypt
27 (HM622138) and Rhizobium leguminosarum bv. viciae STDF-Egypt 19 (HM587713). The best alkaline
tolerant was R. leguminosarum bv. viciae STDF-Egypt 19 (HM587713). The effect of R. leguminosarum
bv. viciae STDF-Egypt 19 and mixture of AMF (Acaulospora laevis, Glomus geosporum, Glomus mosseae
and Scutellospora armeniaca) both individually and in combination on nodulation, nitrogen fixation and
growth of Vicia faba under alkalinity stress were assessed. A significant increase over control in number
and mass of nodules, nitrogenase activity, leghaemoglobin content of nodule, mycorrhizal colonization,
dry mass of root and shoot was recorded in dual inoculated plants than plants with individual inoculation.
The enhancement of nitrogen fixation of faba bean could be attributed to AMF facilitating the
mobilization of certain elements such as P, Fe, K and other minerals that involve in synthesis of nitrogenase
and leghaemoglobin. Thus it is clear that the dual inoculation with Rhizobium and AMF biofertilizer
is more effective for promoting growth of faba bean grown in alkaline soils than the individual treatment,
reflecting the existence of synergistic relationships among the inoculants.

Egyptian soils are generally characterized by slightly alkaline to alkaline pH values (7.5–8.7) which are
mainly due to its dry environment. In arid and semi-arid regions, salts are less concentrated and sodium
dominates in carbonate and bicarbonate forms, which enhance the formation of alkaline soils. Alkaline
soils have fertility problems due to poor physical properties which adversely affect the growth and the
yield of crops. Therefore, this study was devoted to investigating the synergistic interaction of Rhizobium
and arbuscular mycorrhizal fungi for improving growth of faba bean grown in alkaline soil. A total of
20 rhizobial isolates and 4 species of arbuscular mycorrhizal fungi (AMF) were isolated. The rhizobial
isolates were investigated for their ability to grow under alkaline stress. Out of 20 isolates 3 isolates were
selected as tolerant isolates. These 3 rhizobial isolates were identified on the bases of the sequences of
the gene encoding 16S rRNA and designated as Rhizobium sp. Egypt 16 (HM622137), Rhizobium sp. Egypt
27 (HM622138) and Rhizobium leguminosarum bv. viciae STDF-Egypt 19 (HM587713). The best alkaline
tolerant was R. leguminosarum bv. viciae STDF-Egypt 19 (HM587713). The effect of R. leguminosarum
bv. viciae STDF-Egypt 19 and mixture of AMF (Acaulospora laevis, Glomus geosporum, Glomus mosseae
and Scutellospora armeniaca) both individually and in combination on nodulation, nitrogen fixation and
growth of Vicia faba under alkalinity stress were assessed. A significant increase over control in number
and mass of nodules, nitrogenase activity, leghaemoglobin content of nodule, mycorrhizal colonization,
dry mass of root and shoot was recorded in dual inoculated plants than plants with individual inoculation.
The enhancement of nitrogen fixation of faba bean could be attributed to AMF facilitating the
mobilization of certain elements such as P, Fe, K and other minerals that involve in synthesis of nitrogenase
and leghaemoglobin. Thus it is clear that the dual inoculation with Rhizobium and AMF biofertilizer
is more effective for promoting growth of faba bean grown in alkaline soils than the individual treatment,
reflecting the existence of synergistic relationships among the inoculants.

In the present study biodiesel was produced by various fungal species isolated from Egypt using sugarcane molasses as substrate. In the first stage 6 oleaginous fungi, namely, Alternaria alternata, Cladosporium cladosporioides, Epicoccum nigrum, Fusarium oxysporum, Aspergillus parasiticus and Emericella nidulans var. lata were used for lipid production. Subsequent to fungal cultivation on sugarcane molasses the cultures were filtered and biodiesel was prepared by direct esterification of dry fungal biomass. Methyl esters of palmitic, stearic, linoleic and elaidic represented the major components while palmitoleic represented a minor component of biodiesel produced from tested oleaginous fungi. In the second stage, the spent medium of fungal culture was used as the fermentation medium for hydrogen production by Clostridium acetobutylicum ATCC 824. The maximum total H2 yield was obtained with the spent medium of Epicoccum nigrum and Alternaria alternata. The results presented in this study suggest a possibility of interlinking the biodiesel production technology by fungi with hydrogen production by Clostridium acetobutylicum ATCC 824 to exploit the residual sugars in the spent media and therefore increase the economic feasibility of the biofuel production from molasses

In the present study biodiesel was produced by various fungal species isolated from Egypt using sugarcane molasses as substrate. In the first stage 6 oleaginous fungi, namely, Alternaria alternata, Cladosporium cladosporioides, Epicoccum nigrum, Fusarium oxysporum, Aspergillus parasiticus and Emericella nidulans var. lata were used for lipid production. Subsequent to fungal cultivation on sugarcane molasses the cultures were filtered and biodiesel was prepared by direct esterification of dry fungal biomass. Methyl esters of palmitic, stearic, linoleic and elaidic represented the major components while palmitoleic represented a minor component of biodiesel produced from tested oleaginous fungi. In the second stage, the spent medium of fungal culture was used as the fermentation medium for hydrogen production by Clostridium acetobutylicum ATCC 824. The maximum total H2 yield was obtained with the spent medium of Epicoccum nigrum and Alternaria alternata. The results presented in this study suggest a possibility of interlinking the biodiesel production technology by fungi with hydrogen production by Clostridium acetobutylicum ATCC 824 to exploit the residual sugars in the spent media and therefore increase the economic feasibility of the biofuel production from molasses

In the present study biodiesel was produced by various fungal species isolated from Egypt using sugarcane molasses as substrate. In the first stage 6 oleaginous fungi, namely, Alternaria alternata, Cladosporium cladosporioides, Epicoccum nigrum, Fusarium oxysporum, Aspergillus parasiticus and Emericella nidulans var. lata were used for lipid production. Subsequent to fungal cultivation on sugarcane molasses the cultures were filtered and biodiesel was prepared by direct esterification of dry fungal biomass. Methyl esters of palmitic, stearic, linoleic and elaidic represented the major components while palmitoleic represented a minor component of biodiesel produced from tested oleaginous fungi. In the second stage, the spent medium of fungal culture was used as the fermentation medium for hydrogen production by Clostridium acetobutylicum ATCC 824. The maximum total H2 yield was obtained with the spent medium of Epicoccum nigrum and Alternaria alternata. The results presented in this study suggest a possibility of interlinking the biodiesel production technology by fungi with hydrogen production by Clostridium acetobutylicum ATCC 824 to exploit the residual sugars in the spent media and therefore increase the economic feasibility of the biofuel production from molasses

In the present study biodiesel was produced by various fungal species isolated from Egypt using sugarcane molasses as substrate. In the first stage 6 oleaginous fungi, namely, Alternaria alternata, Cladosporium cladosporioides, Epicoccum nigrum, Fusarium oxysporum, Aspergillus parasiticus and Emericella nidulans var. lata were used for lipid production. Subsequent to fungal cultivation on sugarcane molasses the cultures were filtered and biodiesel was prepared by direct esterification of dry fungal biomass. Methyl esters of palmitic, stearic, linoleic and elaidic represented the major components while palmitoleic represented a minor component of biodiesel produced from tested oleaginous fungi. In the second stage, the spent medium of fungal culture was used as the fermentation medium for hydrogen production by Clostridium acetobutylicum ATCC 824. The maximum total H2 yield was obtained with the spent medium of Epicoccum nigrum and Alternaria alternata. The results presented in this study suggest a possibility of interlinking the biodiesel production technology by fungi with hydrogen production by Clostridium acetobutylicum ATCC 824 to exploit the residual sugars in the spent media and therefore increase the economic feasibility of the biofuel production from molasses