The interactions of nanoparticles (NPs) with “protein corona” and live cells are likely to become important in bionanoscience. These interactions play the central role in nanomedicine and nanosafety issues. The protein-adsorption layers located on the surface of colloidal NPs play an important role in their interaction with living cells, so characterization of the protein corona is of the utmost importance for understanding how exposure to NPs affects the biological responses of cells and organisms. This review deals with the interaction of NPs with proteins, live cells and organelles, and considers the proteomics analysis by which these interactions affect cytotoxicity. We offer an overview of the cytotoxicity of different NPs using proteomics analysis. We also review proteomics analysis of natural mineralo-protein NPs. Among the different approaches, proteomics analysis is simple, informative and cheap

Photothermal treatment of graphene oxide (GO) for antibacterial, antifungal and controlling the wound infection treatment using near infrared laser (NIR, Nd-YAG (λ = 1064 nm) were reported. Various pathogenic bacteria (Pseudomonas aeruginosa, Staphylococcus aureus) and fungi (Saccharomyces cerevisiae and Candida utilis) were investigated. The cytotoxicity was measured using the proteomic analysis by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), optical density (OD600), standard microdilution procedures, transmission electron microscopy (TEM) and epifluorescence microscopy. The laser mediated the surface activation of GO offer high efficiency for antifungal and antibacterial. Wide broad cells with various instruments approved that graphene oxide is promising material for nanomedicine in the near future.

Flutamide (2-methyl-N-[4-nitro-3-(trifluoromethyl)phenyl]-propanamide) is a non-steroidal drug that has a specific anti-androgenic activity so that it is used in the treatment of prostate cancer. A simple method for extraction and detection of flutamide from pharmaceutical forms are reported using Soxhlet apparatus combined electrospray ionization tandem mass spectrometry (ESI(−)-MSn, n = 1−4) in negative ion mode. Spectra show a fingerprint peaks corresponding to flutamide. Propanamide moiety plays important role in structure elucidation. The diagnostic ions produced a useful fingerprint maps to identify flutamide in unknown samples. Furthermore, the proposed ions may help in the metabolite studies of the drug. The method proposed here is easy, cheap and applicable for large quantity.

The syntheses and antibacterial activity of AgFeO2 and AgFO2 modified polyethylene glycols (PEGs) are reported. The antibacterial activity is investigated against different pathogenic bacteria for possible water treatment applications. The antibacterial activities are quantified by counting the colonies formed in a petri dish, a method called plate counting, and by measuring the turbidity using optical density at wavelength 600 nm (OD600). AgFeO2 nanoparticles (NPs) offer high antibacterial efficiency and can be easily separated from the solution using a small magnetic bar. The data revealed that higher concentrations led to high potency. PEG not only improves the biocompatibility of AgFeO2 by mitigating the cytotoxicity of the core but also improves the dispersion of the AgFeO2 NPs. Because of their superior cytotoxicity and magnetic property, this antibacterial material may have great potential in biomedical …

Detection of mefenamic acid (M, non-steroidal anti-inflammatory drug, NSAIDs) and its metallodrug was investigated using electrospray ionization mass spectrometry (ESI-MS) and fluorescence spectroscopy. ESI-MS data (500 µL, 1× 10-3 M) revealed high detection sensitivity for the drug and metallodrug. ESI-MS spectra revealed peaks at 242, 580, and 777 Da corresponding to [M+ H]+,[63Cu (MH) 2 (H2O) 2+ H]+, and [56Fe (MH) 3+ H]+, respectively. The metal: mefenamic ratios of ESIMS spectra are in complete agreement with the fluorescence spectroscopy results (1: 2 for Cu (II) and 1: 3 for Fe (III)). ESI is a soft ionization technique that can be used on labile metallo-mefenamic acids and is promising for the detection of these species in environmental samples and biological fluids.

We report on a highly sensitive and remarkably selective fluorometric assay for mercury(II) ion. It is based on the formation of a complex between thymine (T) and Hg(II) to form the [Hg(T)2(H2O)2]n complex on the surface of reduced graphene oxide (rGO). This causes the fluorescence emission of rGO (peaking at 550 nm) to be quenched. Under optimal conditions, quenching is selective and fluorescence intensity is linearly related to the concentration of Hg(II) in the range between 100 and 700 nM. The limit of detection is 5 nM. In addition to its sensitivity and selectivity, the method is simple and direct.

A series of highly porous isoreticular lanthanide-based metal–organic frameworks (LnMOFs) denoted as SUMOF-7I to SUMOF-7IV (SU = Stockholm University; Ln = La, Ce, Pr, Nd, Sm, Eu, and Gd) have been synthesized using tritopic carboxylates as the organic linkers. The SUMOF-7 materials display one-dimensional pseudohexagonal channels with the pore diameter gradually enlarged from 8.4 to 23.9 Å, as a result of increasing sizes of the organic linkers. The structures have been solved by single crystal X-ray diffraction or rotation electron diffraction (RED) combined with powder X-ray diffraction (PXRD). The SUMOF-7 materials exhibit robust architectures with permanent porosity. More importantly, they exhibit exceptionally high thermal and chemical stability. We show that, by inclusion of organic dye molecules, the luminescence properties of the MOFs can be elaborated and modulated, leading to promising …

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One-pot synthesis of cuprous ferrite (CuFeO2) nanoparticles modified with glycerol (glycerol@CuFeO2 NPs) using oligol and reducing agent was reported. The antifungal activities of the nanoparticles for Candida utilis using matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) are also reported. Glycerol@CuFeO2 NPs are synthesized via hydrothermal method and characterized using TEM, SEM, EDX, XRD, FTIR, DLS, and zeta potential. The data revealed that glycerol@CuFeO2 NPs show an efficient ability to degrade contaminants, such as fungi in the aquatic environment. The proteomic analysis of the pathogenic fungus upon addition of different concentrations of glycerol@CuFeO2 NPs and different incubation times reveals the degradation of high molecular weight protein to small proteins or peptides.