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Biodegradation of Palisada perforata (Rhodophyceae) and Sargassum sp.(Phaeophyceae) biomass by crude enzyme preparations from algicolous fungi

Research Abstract
There is a growing demand for the efficient treatment of seaweed biomass and the production of seaweeddegrading enzymes. Fifteen algicolous fungi were isolated from the Red Sea macroalgae in Egypt and were studied for their capability of utilizing Sargassum and Palisada biomass and subsequent production of different thallus-degrading enzymes. Most of the fungi efficiently saccharified the macroalgal extracts that amounted to more than 70 % of the sugars in the unfermented macroalgal extract. Algicolous fungi fermented Sargassum through extracellular fucoidanases and alginases that were negatively correlated as revealed by principal component analysis (PCA), suggesting an antagonistic degradation of Sargassum polysaccharides. Extracellular agarases resulted in efficient fermentation of Palisada biomass. Fungi expressed also amylase and protease activities that were low or nonexistent and biomass-dependent.Amylase showed positive correlation with agarase as indicated by PCA, which suggests that the two enzymes synergistically degrade Palisada biomass. The enzymatic cocktails were also able to release reducing sugars from the powdered macroalgal thalli, indicating the importance of enzymes in the saprophytic growth of fungi. Generally, enzymatic activities and specific activities of fucoidanase, alginase, and agarase in algicolous fungi were higher than reported previously for non-algicolous fungi. Enzymatic activities of the marine brown algal pathogen Lindra thalassiae indicated that infection by this pathogen might occur through alginases. The results shed light into production of less expensive enzymatic mixtures from an understudied group of microorganisms targeting seaweeddegradation. These enzymes have many biotechnological and industrial applications as thallus maceration and protoplast isolation despite bioactivity of obtained oligosaccharides
Research Authors
Mohamed Gomaa & Awatief F. Hifney &
Mustafa A. Fawzy & Ahmed A. Issa &
Khayria M. Abdel-Gawad
Research Journal
Journal of Applied Phycology
Research Pages
pp. 2395–2404
Research Publisher
Springer
Research Rank
1
Research Vol
Vol. 27
Research Website
NULL
Research Year
2014

Biodegradation of Palisada perforata (Rhodophyceae) and Sargassum sp.(Phaeophyceae) biomass by crude enzyme preparations from algicolous fungi

Research Abstract
There is a growing demand for the efficient treatment of seaweed biomass and the production of seaweeddegrading enzymes. Fifteen algicolous fungi were isolated from the Red Sea macroalgae in Egypt and were studied for their capability of utilizing Sargassum and Palisada biomass and subsequent production of different thallus-degrading enzymes. Most of the fungi efficiently saccharified the macroalgal extracts that amounted to more than 70 % of the sugars in the unfermented macroalgal extract. Algicolous fungi fermented Sargassum through extracellular fucoidanases and alginases that were negatively correlated as revealed by principal component analysis (PCA), suggesting an antagonistic degradation of Sargassum polysaccharides. Extracellular agarases resulted in efficient fermentation of Palisada biomass. Fungi expressed also amylase and protease activities that were low or nonexistent and biomass-dependent.Amylase showed positive correlation with agarase as indicated by PCA, which suggests that the two enzymes synergistically degrade Palisada biomass. The enzymatic cocktails were also able to release reducing sugars from the powdered macroalgal thalli, indicating the importance of enzymes in the saprophytic growth of fungi. Generally, enzymatic activities and specific activities of fucoidanase, alginase, and agarase in algicolous fungi were higher than reported previously for non-algicolous fungi. Enzymatic activities of the marine brown algal pathogen Lindra thalassiae indicated that infection by this pathogen might occur through alginases. The results shed light into production of less expensive enzymatic mixtures from an understudied group of microorganisms targeting seaweeddegradation. These enzymes have many biotechnological and industrial applications as thallus maceration and protoplast isolation despite bioactivity of obtained oligosaccharides
Research Authors
Mohamed Gomaa & Awatief F. Hifney &
Mustafa A. Fawzy & Ahmed A. Issa &
Khayria M. Abdel-Gawad
Research Journal
Journal of Applied Phycology
Research Member
Research Pages
pp. 2395–2404
Research Publisher
Springer
Research Rank
1
Research Vol
Vol. 27
Research Website
NULL
Research Year
2014

Biodegradation of Palisada perforata (Rhodophyceae) and Sargassum sp.(Phaeophyceae) biomass by crude enzyme preparations from algicolous fungi

Research Abstract
There is a growing demand for the efficient treatment of seaweed biomass and the production of seaweeddegrading enzymes. Fifteen algicolous fungi were isolated from the Red Sea macroalgae in Egypt and were studied for their capability of utilizing Sargassum and Palisada biomass and subsequent production of different thallus-degrading enzymes. Most of the fungi efficiently saccharified the macroalgal extracts that amounted to more than 70 % of the sugars in the unfermented macroalgal extract. Algicolous fungi fermented Sargassum through extracellular fucoidanases and alginases that were negatively correlated as revealed by principal component analysis (PCA), suggesting an antagonistic degradation of Sargassum polysaccharides. Extracellular agarases resulted in efficient fermentation of Palisada biomass. Fungi expressed also amylase and protease activities that were low or nonexistent and biomass-dependent.Amylase showed positive correlation with agarase as indicated by PCA, which suggests that the two enzymes synergistically degrade Palisada biomass. The enzymatic cocktails were also able to release reducing sugars from the powdered macroalgal thalli, indicating the importance of enzymes in the saprophytic growth of fungi. Generally, enzymatic activities and specific activities of fucoidanase, alginase, and agarase in algicolous fungi were higher than reported previously for non-algicolous fungi. Enzymatic activities of the marine brown algal pathogen Lindra thalassiae indicated that infection by this pathogen might occur through alginases. The results shed light into production of less expensive enzymatic mixtures from an understudied group of microorganisms targeting seaweeddegradation. These enzymes have many biotechnological and industrial applications as thallus maceration and protoplast isolation despite bioactivity of obtained oligosaccharides
Research Authors
Mohamed Gomaa & Awatief F. Hifney &
Mustafa A. Fawzy & Ahmed A. Issa &
Khayria M. Abdel-Gawad
Research Journal
Journal of Applied Phycology
Research Member
Research Pages
pp. 2395–2404
Research Publisher
Springer
Research Rank
1
Research Vol
Vol. 27
Research Website
NULL
Research Year
2014

Spatio-temporal, environmental factors, and host identity shape culturable-epibiotic fungi of seaweeds in the Red Sea, Egypt

Research Abstract
The study of fungal species diversity from marine algae is in its infancy; as now no studies have been carried out on the distribution and diversity of fungi on the surfaces of marine macroalgae where all fungal–algal interactions tend to begin. The aim of this study was to isolate and describe the culturable part of mycobiota associated with the surface of benthic marine macroalgae (epiphytic or epibiotic fungi). This is an important step in understanding their abundance, diversity and factors influencing their variability and composition. The fungal community was dominated by Ascomycetes (89%) with Eurotiales as the most abundant fungal order followed by Capnodiales, Pleosporales, and Hypocreales, while Zygomycetes was less frequent. The nature of occurrence of fungal genera on different macroalgal hosts suggests that a mix of generalists’ framework applies to fungal epiphytes of seaweeds, but the abundance of fungal taxa varied among ecological functional groups of algae, as well as macroalgal taxonomic groups, which imply host filtering. The fungal assemblages were also characterized by temporal variation with variation in temperature, pH, and salinity as the most important abiotic factors. The structure of fungal assemblages showed high beta diversity and low similarity between hosts.
Research Authors
Khayria M. Abdel-Gawad • Awatief F. Hifney •
Ahmed A. Issa • Mohamed Gomaa
Research Journal
Hydrobiologia
Research Pages
37-49
Research Publisher
Springer
Research Rank
1
Research Vol
740
Research Website
NULL
Research Year
2014

Spatio-temporal, environmental factors, and host identity shape culturable-epibiotic fungi of seaweeds in the Red Sea, Egypt

Research Abstract
The study of fungal species diversity from marine algae is in its infancy; as now no studies have been carried out on the distribution and diversity of fungi on the surfaces of marine macroalgae where all fungal–algal interactions tend to begin. The aim of this study was to isolate and describe the culturable part of mycobiota associated with the surface of benthic marine macroalgae (epiphytic or epibiotic fungi). This is an important step in understanding their abundance, diversity and factors influencing their variability and composition. The fungal community was dominated by Ascomycetes (89%) with Eurotiales as the most abundant fungal order followed by Capnodiales, Pleosporales, and Hypocreales, while Zygomycetes was less frequent. The nature of occurrence of fungal genera on different macroalgal hosts suggests that a mix of generalists’ framework applies to fungal epiphytes of seaweeds, but the abundance of fungal taxa varied among ecological functional groups of algae, as well as macroalgal taxonomic groups, which imply host filtering. The fungal assemblages were also characterized by temporal variation with variation in temperature, pH, and salinity as the most important abiotic factors. The structure of fungal assemblages showed high beta diversity and low similarity between hosts.
Research Authors
Khayria M. Abdel-Gawad • Awatief F. Hifney •
Ahmed A. Issa • Mohamed Gomaa
Research Journal
Hydrobiologia
Research Pages
37-49
Research Publisher
Springer
Research Rank
1
Research Vol
740
Research Website
NULL
Research Year
2014

Thermal Radiation, Heat Source/Sink and Work Done by
Deformation Impacts on MHD Viscoelastic Fluid over a
Nonlinear Stretching Sheet

Research Abstract
This work is focused on the effects of heat source/sink, viscous dissipation, radiation and work done by deformation on flow and heat transfer of a viscoelastic fluid over a nonlinear stretching sheet. The similarity transformations have been used to convert the governing partial differential equations into a set of nonlinear ordinary differential equations. These equations are then solved numerically using a very efficient implicit finite difference method. Favorable comparison with previously published work is performed and it is found to be in excellent agreement. The results of this parametric study are shown in several plots and tables and the physical aspects of the problem are highlighted and discussed
Research Authors
F. M. Hady1, R. A. Mohamed2, Hillal M. ElShehabey2,3
Research Department
Research Journal
World Journal of Mechanics,
Research Member
Research Pages
203 - 214
Research Publisher
2014 Scientific Advances Publishers
Research Rank
1
Research Vol
3
Research Year
2013
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