NULL

NULL

NULL

NULL

NULL

A simple and sensitive high performance thin-layer chromatographic method (HPTLC) was developed and validated for the analysis of two binary mixtures of antiepileptic drugs. The first mixture consists of eslicarbazepine acetate (ESL) and oxcarbazepine (OXC). The second one consists of carbamazepine (CBZ) and OXC. These drugs have intercalated metabolic pathways, which was the prime reason for the development of the proposed analytical methodology for their determination. The two mixtures were separated on pre-coated silica gel HPTLC plates G60 F254 utilizing a mixture of n-hexane: methylene chloride: ethanol: glacial acetic acid in the ratio (50:40:10:0.1 v/v/v/v) as a mobile phase. The first mixture was detected at 217 nm, while the second one was detected at 265 nm. The achieved sensitivity is very high as reflected by the limit of detection values ranging from 5.03 to 12.60 ng/spot for the studied drugs. The developed method is the first HPTLC method for separation of these drugs in their dosage forms and in human plasma. Good recovery results were obtained from pharmaceutical tablets in the range from 97.75 to 100.40% without any interference from co-formulated excipients. In addition, high recovery results from plasma samples after utilizing salting out assisted liquid-liquid extraction technique (SALLE) were obtained, which ranges from 88.17 to 101.95%, ensuring the efficiency of the proposed approach. Furthermore, the developed method was successfully applied to detect CBZ clearly in real patients' plasma without any interference from matrix components.

A simple and sensitive high performance thin-layer chromatographic method (HPTLC) was developed and validated for the analysis of two binary mixtures of antiepileptic drugs. The first mixture consists of eslicarbazepine acetate (ESL) and oxcarbazepine (OXC). The second one consists of carbamazepine (CBZ) and OXC. These drugs have intercalated metabolic pathways, which was the prime reason for the development of the proposed analytical methodology for their determination. The two mixtures were separated on pre-coated silica gel HPTLC plates G60 F254 utilizing a mixture of n-hexane: methylene chloride: ethanol: glacial acetic acid in the ratio (50:40:10:0.1 v/v/v/v) as a mobile phase. The first mixture was detected at 217 nm, while the second one was detected at 265 nm. The achieved sensitivity is very high as reflected by the limit of detection values ranging from 5.03 to 12.60 ng/spot for the studied drugs. The developed method is the first HPTLC method for separation of these drugs in their dosage forms and in human plasma. Good recovery results were obtained from pharmaceutical tablets in the range from 97.75 to 100.40% without any interference from co-formulated excipients. In addition, high recovery results from plasma samples after utilizing salting out assisted liquid-liquid extraction technique (SALLE) were obtained, which ranges from 88.17 to 101.95%, ensuring the efficiency of the proposed approach. Furthermore, the developed method was successfully applied to detect CBZ clearly in real patients' plasma without any interference from matrix components.

A simple and sensitive high performance thin-layer chromatographic method (HPTLC) was developed and validated for the analysis of two binary mixtures of antiepileptic drugs. The first mixture consists of eslicarbazepine acetate (ESL) and oxcarbazepine (OXC). The second one consists of carbamazepine (CBZ) and OXC. These drugs have intercalated metabolic pathways, which was the prime reason for the development of the proposed analytical methodology for their determination. The two mixtures were separated on pre-coated silica gel HPTLC plates G60 F254 utilizing a mixture of n-hexane: methylene chloride: ethanol: glacial acetic acid in the ratio (50:40:10:0.1 v/v/v/v) as a mobile phase. The first mixture was detected at 217 nm, while the second one was detected at 265 nm. The achieved sensitivity is very high as reflected by the limit of detection values ranging from 5.03 to 12.60 ng/spot for the studied drugs. The developed method is the first HPTLC method for separation of these drugs in their dosage forms and in human plasma. Good recovery results were obtained from pharmaceutical tablets in the range from 97.75 to 100.40% without any interference from co-formulated excipients. In addition, high recovery results from plasma samples after utilizing salting out assisted liquid-liquid extraction technique (SALLE) were obtained, which ranges from 88.17 to 101.95%, ensuring the efficiency of the proposed approach. Furthermore, the developed method was successfully applied to detect CBZ clearly in real patients' plasma without any interference from matrix components.

A simple and sensitive high performance thin-layer chromatographic method (HPTLC) was developed and validated for the analysis of two binary mixtures of antiepileptic drugs. The first mixture consists of eslicarbazepine acetate (ESL) and oxcarbazepine (OXC). The second one consists of carbamazepine (CBZ) and OXC. These drugs have intercalated metabolic pathways, which was the prime reason for the development of the proposed analytical methodology for their determination. The two mixtures were separated on pre-coated silica gel HPTLC plates G60 F254 utilizing a mixture of n-hexane: methylene chloride: ethanol: glacial acetic acid in the ratio (50:40:10:0.1 v/v/v/v) as a mobile phase. The first mixture was detected at 217 nm, while the second one was detected at 265 nm. The achieved sensitivity is very high as reflected by the limit of detection values ranging from 5.03 to 12.60 ng/spot for the studied drugs. The developed method is the first HPTLC method for separation of these drugs in their dosage forms and in human plasma. Good recovery results were obtained from pharmaceutical tablets in the range from 97.75 to 100.40% without any interference from co-formulated excipients. In addition, high recovery results from plasma samples after utilizing salting out assisted liquid-liquid extraction technique (SALLE) were obtained, which ranges from 88.17 to 101.95%, ensuring the efficiency of the proposed approach. Furthermore, the developed method was successfully applied to detect CBZ clearly in real patients' plasma without any interference from matrix components.

A simple and highly sensitive ultra-high-performance liquid chromatographic–diode array (UHPLC-DAD) detection method was developed and validated for the simultaneous estimation of levetiracetam (LEV) and lacosamide (LAC). It was clinically proven that the combination of LEV and LAC exhibits a synergistic effect against refractory seizures in mice, which was the motivation for the analysis of this binary mixture both in bulk and in human urine samples. The binary mixture was resolved on a Hypersil BDS C18 analytical column, utilizing a mobile phase of 0.050 mol L−1 phosphate buffer (pH 5.60), methanol and acetonitrile in the ratio (80:10:10 v/v/v) using catechol as an internal standard. The mobile phase was pumped at a flow rate of 1.2 mL min−1 with diode array detection at 205 nm for both drugs and 270 nm for IS. Calibration curves were linear with correlation coefficient >0.9990 over the studied concentration range of 0.1–70.0 μg mL−1 for both drugs. The developed method was reproducible with low relative standard deviation values for intra- and inter-day precision (2.0%). Both drugs were determined in bulk, pharmaceutical formulations and human urine samples without any interference from complex matrices.