Aim: Hepatotoxicity is the most serious adverse effect of rifampicin (RIF). We aimed to investigate the potential hepatoprotective effect of mannose-functionalized poly(lactic-co-glycolic acid)(PLGA)/RIF nanoparticles (NPs) in rats as a possible promising approach to minimize RIF-induced hepatotoxicity. Ma- terials & methods: Mannose-functionalized PLGA/RIF NPs were fabricated and characterized in vitro, then the hepatoprotective effect of optimized NPs was studied on rat and cell culture models. Results: Follow- ing intraperitoneal administration of RIF NPs into rats, highly significant differences in levels of serum transaminases and oxidative stress markers, associated with significant differences in expression of Bax and Bcl-2 genes between NP- and free RIF-treated groups, revealing the hepatoprotective potential of NPs. Conclusion: RIF NPs may represent a promising therapeutic approach for tuberculosis via reducing dose frequency and consequently, RIF-induced hepatotoxicity.

Aim: Hepatotoxicity is the most serious adverse effect of rifampicin (RIF). We aimed to investigate the potential hepatoprotective effect of mannose-functionalized poly(lactic-co-glycolic acid)(PLGA)/RIF nanoparticles (NPs) in rats as a possible promising approach to minimize RIF-induced hepatotoxicity. Ma- terials & methods: Mannose-functionalized PLGA/RIF NPs were fabricated and characterized in vitro, then the hepatoprotective effect of optimized NPs was studied on rat and cell culture models. Results: Follow- ing intraperitoneal administration of RIF NPs into rats, highly significant differences in levels of serum transaminases and oxidative stress markers, associated with significant differences in expression of Bax and Bcl-2 genes between NP- and free RIF-treated groups, revealing the hepatoprotective potential of NPs. Conclusion: RIF NPs may represent a promising therapeutic approach for tuberculosis via reducing dose frequency and consequently, RIF-induced hepatotoxicity.

Aim: Hepatotoxicity is the most serious adverse effect of rifampicin (RIF). We aimed to investigate the potential hepatoprotective effect of mannose-functionalized poly(lactic-co-glycolic acid)(PLGA)/RIF nanoparticles (NPs) in rats as a possible promising approach to minimize RIF-induced hepatotoxicity. Ma- terials & methods: Mannose-functionalized PLGA/RIF NPs were fabricated and characterized in vitro, then the hepatoprotective effect of optimized NPs was studied on rat and cell culture models. Results: Follow- ing intraperitoneal administration of RIF NPs into rats, highly significant differences in levels of serum transaminases and oxidative stress markers, associated with significant differences in expression of Bax and Bcl-2 genes between NP- and free RIF-treated groups, revealing the hepatoprotective potential of NPs. Conclusion: RIF NPs may represent a promising therapeutic approach for tuberculosis via reducing dose frequency and consequently, RIF-induced hepatotoxicity.

Herbal medicines are widely used worldwide and much appreciated because of their fewer side effects and the ability to fight diseases at the root cause. Active ‘phyto’ ingredients require a scientific approach and a mechanism to distribute components at the target site for better therapeutic results. Nanotechnology, on the other hand, has created new hope for cancer treatment but is still far from being proven in clinical settings. This article combines a unique approach to synthesis with the use of Pleurotus sajor-caju, followed by microwave irritation of silver and gold nanoparticles that ensures the capping of the active phyto ingredient and further enhances the effects of nanomedicine to fight colon cancer, thus opening a new era of what we call herbonanoceutics. The article also compares the characteristics and properties of silver (Au) and gold (Ag) nanoparticles synthesized by an in house developed novel microwave-assisted rapid green synthesis method. The as-prepared Ag NPs and Au NPs were compared using ultraviolet-visible spectroscopy (UV-Vis), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and energy dispersive spectroscopy (EDS). Our comparative study revealed that both assemblies display face-centred cubic structures (FCCs) and are nanocrystalline in nature. The advantage of the approach was that the sizes of gold and silver were identical in range with a similar distribution pattern. This has helped us to study the activity against colon cancer cell line (HCT-116) without incoherence since size plays a key role in the application. More specifically, morphological changes, cell viability, the production of reactive oxygen species (ROS) and the fragmentation of DNA have been further reported to assess better the results obtained with the two metals. Our results suggest that the newly adopted synthesis method may ensure the dual benefits from phyto ingredients which further enhances the effectiveness of advanced nanomedicine.

Herbal medicines are widely used worldwide and much appreciated because of their fewer side effects and the ability to fight diseases at the root cause. Active ‘phyto’ ingredients require a scientific approach and a mechanism to distribute components at the target site for better therapeutic results. Nanotechnology, on the other hand, has created new hope for cancer treatment but is still far from being proven in clinical settings. This article combines a unique approach to synthesis with the use of Pleurotus sajor-caju, followed by microwave irritation of silver and gold nanoparticles that ensures the capping of the active phyto ingredient and further enhances the effects of nanomedicine to fight colon cancer, thus opening a new era of what we call herbonanoceutics. The article also compares the characteristics and properties of silver (Au) and gold (Ag) nanoparticles synthesized by an in house developed novel microwave-assisted rapid green synthesis method. The as-prepared Ag NPs and Au NPs were compared using ultraviolet-visible spectroscopy (UV-Vis), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and energy dispersive spectroscopy (EDS). Our comparative study revealed that both assemblies display face-centred cubic structures (FCCs) and are nanocrystalline in nature. The advantage of the approach was that the sizes of gold and silver were identical in range with a similar distribution pattern. This has helped us to study the activity against colon cancer cell line (HCT-116) without incoherence since size plays a key role in the application. More specifically, morphological changes, cell viability, the production of reactive oxygen species (ROS) and the fragmentation of DNA have been further reported to assess better the results obtained with the two metals. Our results suggest that the newly adopted synthesis method may ensure the dual benefits from phyto ingredients which further enhances the effectiveness of advanced nanomedicine.

In recent decades, H2O2 has been promoted as a health indicator because its moderate to high levels can cause some health problems. Herein, we developed a new fluorescent nanoprobe for rapid, selective and sensitive detection of H2O2. The fluorescent nanoprobe is composed of fluorescein dye (FLS) as a fluorescent probe and MnO2 nanosheets (MnO2 NS) as a quencher. In this study, H2O2 can reduce MnO2 NS in the synthesized composite and release FLS, causing sufficient recovery of fluorescent signal related to the concentration of H2O2. The nanoprobe, with λex/λem at 495/515 nm, has a linear range of 0.04–30 μM, with a limit of detection (LOD) of 7.5 nM and a limit of quantitation (LOQ) of 21 nM. The mean relative standard deviation (RSD) was 2.6% and the applicability of the method was demonstrated by the determination of H2O2 in water and cosmetic samples.

In recent decades, H2O2 has been promoted as a health indicator because its moderate to high levels can cause some health problems. Herein, we developed a new fluorescent nanoprobe for rapid, selective and sensitive detection of H2O2. The fluorescent nanoprobe is composed of fluorescein dye (FLS) as a fluorescent probe and MnO2 nanosheets (MnO2 NS) as a quencher. In this study, H2O2 can reduce MnO2 NS in the synthesized composite and release FLS, causing sufficient recovery of fluorescent signal related to the concentration of H2O2. The nanoprobe, with λex/λem at 495/515 nm, has a linear range of 0.04–30 μM, with a limit of detection (LOD) of 7.5 nM and a limit of quantitation (LOQ) of 21 nM. The mean relative standard deviation (RSD) was 2.6% and the applicability of the method was demonstrated by the determination of H2O2 in water and cosmetic samples.

An innovative label free electrochemical aptasensor was developed for the analysis of oxaliplatin (OXAL) for the first time. The DNA oligonucleotide (aptamer) was successfully fabricated, by covalently attaching the amino terminus of the functional DNA on the glassy carbon electrode (GCE) surface modified with reduced graphene oxide (rGO) and multi-walled carbon nanotubes (MWCNTs) loaded with AuPd nanoparticles (AuPd NPs@rGO/MWCNTs/GCE). The stepwise assembly process of aptasensor on AuPd NPs@rGO/MWCNTs/GCE was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The aptamer-OXAL complex formation led to inhibition the electron transfer of Fe(CN)63−/4- on the electrode interface, which was clearly observed by decreasing the peak current of the redox probe. Furthermore, we managed to quantitatively measure OXAL by adding different concentrations of OXAL, while monitoring the decrease of differential pulse voltammogram (DPV) responses of the redox probe. Under the optimized conditions, the electrochemical aptasensor exhibited a linear range of 0.1–170.0 nmol L−1 with LOD of 60.0 pmol L−1. Next, we successfully applied the aptasensor calibrated system to determine OXAL in pharmaceutical injection and human biological samples.

In this study, an electrochemical aptamer based sensor (aptasensor) was proposed for specific recognition of histamine (HIS). The electrochemical aptasensor based on fabrication of glassy carbon electrode (GCE) with molecular imprinted polymer (MIP) and DNA aptamers on gold nanoparticles (AuNPs) and carboxylated carbon nanotubes (cCNTs) (MIP-apta/AuNPs/cCNTs/GCE). The aptasensor exhibits high selectivity towards HIS detection as it has two recognition elements which are MIP cavities and aptamer interaction. Upon exposure of MIP-apt/AuNPs/cCNTs/GCE to HIS, the current of redox probe was decreased that depends on the template (HIS) concentration. The effects of aptamer concentration, incubation time, pH and AuNPs electro-deposition time were optimized. Differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques were used to analyze HIS in complicated matrices. Favorable performance of MIP-apt/AuNPs/cCNTs/GCE was achieved with linearity ranges of 0.46–35 nmol L−1 and 0.35–35 nmol L−1 with limits of detection (LODs, S/N = 3) of 0.15 nmol L−1 and 0.11 nmol L−1 using DPV and EIS, respectively. The fabricated aptasensor displayed high selectivity, desirable reproducibility and stability. The MIP-apt/AuNPs/cCNTs/GCE was used to detect HIS in human plasma and canned tuna samples with good recoveries % and RSDs %.

In this study, an electrochemical aptamer based sensor (aptasensor) was proposed for specific recognition of histamine (HIS). The electrochemical aptasensor based on fabrication of glassy carbon electrode (GCE) with molecular imprinted polymer (MIP) and DNA aptamers on gold nanoparticles (AuNPs) and carboxylated carbon nanotubes (cCNTs) (MIP-apta/AuNPs/cCNTs/GCE). The aptasensor exhibits high selectivity towards HIS detection as it has two recognition elements which are MIP cavities and aptamer interaction. Upon exposure of MIP-apt/AuNPs/cCNTs/GCE to HIS, the current of redox probe was decreased that depends on the template (HIS) concentration. The effects of aptamer concentration, incubation time, pH and AuNPs electro-deposition time were optimized. Differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques were used to analyze HIS in complicated matrices. Favorable performance of MIP-apt/AuNPs/cCNTs/GCE was achieved with linearity ranges of 0.46–35 nmol L−1 and 0.35–35 nmol L−1 with limits of detection (LODs, S/N = 3) of 0.15 nmol L−1 and 0.11 nmol L−1 using DPV and EIS, respectively. The fabricated aptasensor displayed high selectivity, desirable reproducibility and stability. The MIP-apt/AuNPs/cCNTs/GCE was used to detect HIS in human plasma and canned tuna samples with good recoveries % and RSDs %.