A ratiometric-based fluorescence emission system was proposed for the determination of sulfide. It consists of blue emissive graphene quantum dots (GQDs) and self-assembled thiolate-protected gold nanoclusters driven by aluminum ion (Al³⁺@GSH-AuNCs). The two types of fluorophores are combined to form a ratiometric emission probe. The orange emission of Al³⁺ @GSH-AuNCs at 624 nm was quenched in the presence of sulfide ion owing to the strong affinity between sulfide and Au(I), while the blue GQDs fluorescence at 470 nm remained unaffected. Interestingly, the Al³⁺@GSH-AuNCs and GQDs were excited under the same excitation wavelength (335 nm). The response ratios (F₄₇₀/F₆₂₄) are linearly proportional to the sulfide concentration within the linear range of 0.02–200 µM under the optimal settings, with a limit of detection (S/N = 3) of 0.0064 µM. The proposed emission probe was applied to detect sulfide ions in tap water and wastewater specimens, with recoveries ranging from 95.3% to 103.3% and RSD% ranging from 2.3% to 3.4%, supporting the proposed method's accuracy.

A simple fluorescence method is described for measuring rutin dependent on the nitogen-doped carbon dots
(NCDs) prepared via simple pyrolysis method from chicken feet biowaste. The as-fabricated NCDs have unique
advantages including cost-effectiveness and high quantum yield (42.9 %). The as-prepared NCDs explore an
optimal emission band at 430 nm following exciting NCDs at 330 nm. Addition of rutin to blue-emissive NCDs
quenched their fluorescence emission by inner-filtration effect (IFE) and static quenching. Under optimized
conditions, the fluorescence responses increased as the rutin amount was raised from 100 to 1000 nmol/L with
5.3 nmol/L as a detection limit (S/N = 3). The probe selectivity was improved by adding bovine serum albumin
(BSA), which binds other structurally-related compounds (other flavonoids). The as-synthesized NCDs exhibited
some advantages towards rutin detection such as: lower LOD value, satisfactorily reproducibility, simplicity,
rapidity, selectivity, and stability. The sensing probe was efficiently utilized for determining rutin in different
real samples with acceptable results. The sensor offers an efficient biowaste-based approach for the determination
of (bio) molecules.

A highly responsive and selective coordinated molecularly imprinted polymer (MIP)-dependent ratiometric
electrochemical platform for ciprofloxacin (CIPX) detection was developed. It consists of porous carbon modified
with heteroatoms (N, S@C) built on pencil graphite electrode (PGE) surface. The N, S@C/PGE was then modified
using silver nanoparticles (AgNPs), which were employed to boost conductivity and surface area of the electrode,
as well as an internal reference signal. Molecularly imprinted film was built on AgNPS/N, S@C/PGE surface in
the existence of template (CIPX) via electro-deposition of Cu (II)-coordinated pyrrole-3-carboxylic acid. The
detection of CIPX is based on the measurement of ICIPX/IAgNPs ratios using differential pulse voltammetry (DPV),
which were linearly proportional to CIPX concentration in the range of 0.13–650 nM with LOD (S/N = 3) of 38
pM. The as-fabricated coordinated MIP-dependent ratiometric electrochemical platform exhibited many advantages
including low detection limit, long-term stability, detection reliability, and good reproducibility/
selectivity. The electrochemical probe was efficiently used for the analysis of CIPX-containing specimens with
recoveries % in the range of 97.6–101.6 % with RSDs % in the range of 2.6–3.2 %, proving accuracy of the
platform.

Herein, new carbon dots doped with nitrogen (N.C-dots) were fabricated from chicken feet bio-waste via facile pyrolysis method. It was utilized to determine diosmin (DIS) fluorometrically and electrochemically (dual-mode). Its fluorescence emission was decreased after DIS addition as a result of inner-filtration effect (IFE). In addition, DIS exhibited a redox activity on the carbon paste electrode (CPE) decorated with N.C-dots (N.C-dots/CPE), enabling its detection via electrochemical method. Different techniques were used to characterize the sensing interfaces. There was a linear decrease in the fluorescence emission of N.C-dots at λ_em. = 430 nm with increasing the amount of DIS over the range of 0.01–0.6 µg mL⁻¹, while there was a linear increase in the oxidation current (Ipa) of DIS with increasing its amount over the range of 0.1–12 µg mL⁻¹. The detection limits (LODs) were calculated to be 3.12 ng mL⁻¹, and 32.91 ng mL⁻¹ for the fluorometric and electrochemical methods, respectively. The as-synthesized sensor possesses many advantages including simplicity, cost-effectiveness, long-term stability, excellent sensitivity, and good selectivity. These advantages enabled the sensor(s) to be applied successfully for the dual-mode detection of DIS in human plasma samples with acceptable results.

This work presents a novel approach for the accurate determination of naringin (NAR) in grapefruit juice. In the proposed method, N-doped carbon dots were employed, which were synthesized via hydrothermal synthesis using eggplant peel food waste as a green precursor. Detection of NAR relied on measuring the probe's emission response, which exhibited a decrease at 430 nm in the presence of NAR. The bitterness caused by NAR in grapefruit juice significantly impacts customer acceptability and can lead to economic losses if its levels exceed a certain threshold. It was found that the fluorescence quenching method showed excellent linearity in the concentration range of 0.0–2500.0 ng mL⁻¹ (R² = 0.9990), with a remarkable limit of detection(S/N = 3) of 16.37 ng mL⁻¹. Furthermore, selective extraction of NAR from grapefruit juice samples was achieved using Amberlite IRA-400 ion exchange resin. The adsorption parameters were carefully selected, and adsorption isotherm models were evaluated to determine the adsorption mechanism. The efficacy of the developed method was demonstrated by its successful application in determining NAR levels in grapefruit juice at various stages of maturity. This study demonstrates the potential of the proposed method as a reliable and efficient tool for accurately determining NAR in grapefruit juice.

A novel and simple hybrid sensor was constructed for the electrochemical sensing of ciprofloxacin (CPX) and metanil yellow (MEY). The sensor consists of fired brick microparticles (FB) modified with a thin film of poly(pyrrole) (PPy), which were used as a modifier for carbon paste electrode (CPE). Adding HCl to FB (which is predominantly made of Fe₂O₃) resulted in the release of Fe³⁺ ions, which aided in the oxidative polymerization of pyrrole monomer. The polar groups and aromatic rings found in the composition of PPy@FB provide more anchoring sites for adsorption of CPX and MEY, facilitating their electro-chemical oxidation. Under optimum conditions, the anodic peak currents (Ipa) showed a linear increase with increasing the concentration of CPX over the concentration range of 0.011–16.0 µmol/L and MEY in the range of 0.012–16.0 µmol/L. Limit of detections (LODs, S/N = 3) were calculated as 0.0035 µmol/L and 0.0042 µmol/L for CPX in milk and water samples, respectively. LOD for MEY in turmeric and water samples were 0.0032 µmol/L and 0.0036 µmol/L, respectively. The advantages of the as-fabricated sensor compromise greenness, simplicity, low-cost, reproducibility, sensitivity, selectivity, and adequate stability. The fabricated sensor was efficiently applied to detect CPX and MEY in different matrices including industrial water, milk, and turmeric samples with satisfactory recoveries % and low RSDs values.