Terbium based cyclic voltammetric (CV) method was developed for analysis of imipenem (IMP) using pencil graphite electrode (PGE). Firstly, complexation process was confirmed and evaluated via spectrofluorimetric approach. Then CV method was developed for the determination of IMP for the first time. The oxidation peak current was found to be linear in the range of 15–70 μM. The limits of detection and quantitation (LOD and LOQ) were found to be 2.44× 10−6 and 7.41× 10−6 M, respectively. The methodwas validated according to ICH guidelines. Furthermore, the method was applied successfully for the analysis of intact IMP in presence of either its potential hydrolytic degradation products or concomitantly administrated cilastatin. The proposed method was also applied successfully to bulk powder, dosage forms and biological samples.