From the methanolic extract of the leaves of Juglans nigra L. (Black Walnut) Family Juglandaceae (Walnut family), kaempferol-3-O-ß-glucoside quercetin-3-O-ß-glucoside and quercetin-3-O- ß-glucoside-6``-ethyl ester were isolated for the first time from the family Juglandaceae, in addition to stigmasterol-3-O-ß-glucoside. The structures were established on the basis of UV, MS and NMR (1H, 13C and DEPT) spectroscopic data. Moreover, the hypotensive and toxicological studies were done.