This paper presents the one‐pot reaction synthesis of coordination calcium and strontium compounds [Ca(L)2(H2O)2 1 and Sr(L)2(H2O)2 2] with the 2‐(4‐ chlorophenylamino) benzoic acid ligand (HL = C13H10NO2Cl). The complexes were obtained in good yields, and their structures were corroborated according to their CHN, spectroscopic (IR and UV/Vis.) and solution molar conductivity data as the latter confirmed their molecular nature. Additionally, the powder X‐ray diffraction pattern of the complexes was used to determine the cell parameters and the hkl indices together with their corresponding 2θ values. The microbial resistance against the currently available antifungal agents requires searching for new antifungal compounds. Promising fungicidal activities of these coordination complexes were determined against the Fusarium corn disease as complexes 1 and 2 at 30°C inhibited the grain pathogenicity by 70% and 66%, respectively. Moreover, 1 and 2 retarded the amylase enzymes that are responsible for the pathogenicity by 35.13% and 26.22% at 30°C.