The Role of CD44 Cancer Stem Cell Marker in the Development and Progression of Lymph Node Metastasis in Oral Squamous Cell Carcinoma

Research Abstract

Abstract

Introduction: Oral squamous cell carcinoma (OSCC) management is challenging due to high tendency of local invasion and metastasis. Cancer stem cells hold high significance as they have self-renewal ability, which further allows cancer progression and metastasis. Hence, it is crucial to evaluate different specialised markers for stem cells, such as CD44, to detect their role in tumour metastasis. Flow cytometry (FCM) offers a quick and automated assessment of ploidy status and cell proliferation of the neoplasm by resolving the nuclear DNA contents.

Aim of the study: The objective of this paper is to evaluate the CD44 expression and analyse DNA content by FCM to predict the expansion of lymph node metastasis in patients with OSCC.

Material and Methods: 50paraffin-embedded tissues of metastatic and non-metastatic lymph node OSCC were immuno stained by CD44 for assessing cancer stem cell activity in each lesion. Furthermore, each selected tissue underwent flow cytometric analysis to demonstrate the DNA activity between the tested groups.

Results: The CD44 expression in OSCCs showed a marked difference between the metastatic and non-metastatic lymph node cases. Furthermore, flow cytometric analysis of the DNA parameters between the tested groups revealed a powerful difference of DNA ploidy. The S-phase fraction (SPF) between the groups showed no compelling result. All specimens had a higher CD44 expression, aneuploid DNA content and high SPF, which demonstrated deposits of cervical metastatic lymph nodes.

Conclusions: The CD44 and the flow cytometric analysis of the DNA ploidy correlation offer a significant prediction to determine the OSCC competence.

Research Authors
Ahmed M. Hussein1 , Asmaa M. Zahran2 , Mohamed Badawy3 , Hany G. Gobran4 , Mohamed F. Edrees5 , Enas M. Omar6*
Research Date
Research Department
Department of Oral Biology
Research File
TheRoleofCD44CancerStemCellMarker.pdf (2 MB)
Research Journal
International Journal of Dentistry and Oral Science (IJDOS)
Research Member
Mohammed Badawy Abdel Hamied Ali
Research Pages
4917-4922
Research Publisher
International Journal of Dentistry and Oral Science (IJDOS)
Research Rank
International
Research Vol
8
Research Website
https://www.researchgate.net/publication/357168072
Research Year
2021

Advanced-Platelet-Rich Fibrin Combination with Metformin for Surgical Correction of Periodontal Intrabony Defects: A Randomized Double Blinded Clinical Trial

Research Abstract

ABSTRACT

Objective: This trial preformed to evaluate clinically and radiographically, the efficacy of growth factors producing/inducing materials; Advanced-Platelet-Rich Fibrin (A-PRF) and Metformin (MF) respectively, in the surgical treatment of intrabony periodontal defects.

Methods: Forty-eight systemically healthy chronic periodontitis patients with intrabony defect were divided equally into 4 groups. First Group, patients treated by open flap debridement (OFD). Second Group, patients treated by OFD + 1% MF gel. Third Group, patients treated by A-PRF, which inserted into the intra bony defect (IBD). Fourth Group treated by (1% MF + A-PRF). Parameters were gathered at baseline 6 and 9 months.

Results: The reduction in probing depth (PD) and clinical attachment level (CAL) was greater in the A-PRF + 1% MF patients group than other groups. Combination of A-PRF + 1% MF showed statistically significant reduction of IBD greater than all other groups.

Conclusions: Usage of combination of A-PRF + 1% MF seems to be superior in gaining bone than surgical treatment by OFD, OFD + A-PRF or OFD + MF only

Research Authors
Asem M. Kamel 1 , Ahmed O. A. Abdelrahman 2 , Hany G. Gobran3 , Hesham A . Dameer4 , Mohamed Badawy4 , Ahmed M. Hussein 5
Research Date
Research Department
Department of Oral Biology
Research File
OMX-Volume 12-Issue 4- Page 267-277.pdf (486.85 KB)
Research Journal
Egyptian Journals
Research Member
Mohammed Badawy Abdel Hamied Ali
Research Pages
267-277
Research Publisher
Egyptian Journals
Research Rank
Local
Research Vol
12
Research Website
https://journals.ekb.eg/article_235387.html
Research Year
2021

Digital Egypt Pioneers Initiative DEPI


The Digital Egypt Pioneers Initiative (DEPI) is a program launched by the Ministry of Communications and Information Technology (MCIT) in Egypt. It aims to cultivate highly skilled and innovative individuals who can lead the charge in the country's digital transformation.

 

Here are some key features of DEPI:

  • Focus on cutting-edge technologies: DEPI provides training in various in-demand fields like artificial intelligence, cloud computing, cybersecurity, and data science.
  • Skills development and leadership: The program goes beyond technical skills, offering training in leadership, management, communication, and entrepreneurship.
  • Collaboration with industry leaders: DEPI partners with international and local tech companies to offer hands-on experience and practical learning opportunities.
  • Multiple tracks and scholarships: The program offers different tracks catering to specific needs and career aspirations, with scholarships available for qualified candidates.
  • Focus on youth empowerment: DEPI prioritizes equipping young Egyptians with the necessary skills and knowledge to thrive in the digital economy.

To register, visit the program website: https://debi.gov.eg/

 

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اعلان عن عقدالمؤتمر الدولي الثاني عشر للتنمية والبيئة في الوطن العربي .

‎Assiut University will host the 12th International Conference For Development and Environment in the Arab World
‎“Sustainable Development and Confronting‏ ‏Environmental ‎& Climate Changes...... Challenges and Solutions"‎, during
‎25-27 February 2024,


under the Auspices of His Excellency
Prof.Dr. Mohamed Ayman Ashour
Minister of Higher Education and Scientific Research

Her Excellency
Dr. Yasmine Fouad
Minister of Environment


His Excellency
Major General. Essam Saad
Governor Of Assiut

His Excellency
‎ Prof.Dr. Ahmed Kamal El-Minshawy
President of Assiut University

Conference Chairman
Prof. Dr. Mahmoud Ahmed Abdel-Aleem
Vice-President for Community Services and Environmental Affairs

Conference Secretary General
Prof. Dr-Ing. Ahmed Hamza Hossieny
Director of Assiut University Center for Environmental Studies and Research

Conference Coordinator
Prof. Dr.- Ing. Adel Abdou H. Ahmed
Director of Assiut University Center for Sustainable Development
 

 

 

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Dentistry Honors October and November 2023 Employees of the Month

Prof./ Safaa Tohamy, Dean of the Faculty, along with Prof/ Heba Rashed, Vice Dean for Community Services and Environmental Development, Prof/ Muhammad Badawy, Vice Dean for Education and Student Affairs, and Mr. Montaser Hussien, Moderator of the Faculty, honored distinguished employees in October and November on Monday December 18,2023, for their outstanding work.  

Winners of the Employee of October are:

Alshimaa Gamal (Quality Assurance Unit)

Ahmad Hassan Ahmad (Youth Welfare Administration)

Winners of the Employee of November are:

Rasha Makram Hanin (Student Affairs Office)

Muhammad Saber (Biology Department)

The Faculty of Dentistry wishes them all the best.

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Dentistry Honors Student Participants in First Scientific Conference

The Faculty of Dentistry would like to express its sincere gratitude to the first- and second-year students who participated in the first scientific conference entitled, “Future Dentistry by Future Dentists, that was held on December 25, 2023.

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