Khirshyat 1.0 is a simple, dynamic and informative micro-program designed mainly to provide scientists, researchers and students in research systems and universities in developing countries, a useful tool on some molecular biology protocols. The program includes the most commonly used molecular markers (AFLP, SRAP and RAPD), PCR optimization, oligonucleotide properties, calculations of concentration and quantity using the molecular weight of most commonly used chemicals, plant DNA extraction, DNA concentration and purity, primer preparation, polyacrylamide gel electrophoresis and silver nitrate staining protocols. It accelerates the process of preparing stocks, buffers, solutions as well as provides important tips and general information with simple illustrations about the most common protocols. The program and documentation are freely available online