Rotavirus ribonucleic acid was extracted from 16 fecal samples of the serologically positive diarrheic calves using Latex agglutination test (LAT) and Immunochrmatographic assay (ICA). The extracted RNA was submitted to Reverse transcriptase polymerase chain reaction (RT-PCR) to detect VP7 and VP4 genes and the positive samples were 100% (16/16) and 81.25% (13/16), respectively. The amplified products were subjected to G and P-genotyping by semi-nested multiplex PCR using of G6, G8 and G10 genotyping and P1, P5 and P11 genotyping primers, respectively. G6 was detected in 10 (62.50%) of 16 samples and G10 was diagnosed in 5 (31.25%) of 16 samples and one (6.25%) sample did not react with any G primer used. P5 was detected in 9 (56.25%) of 16 samples, P11 was diagnosed in 3 (18.75%) of 16 samples, mixed infection with P5+P11 was observed in 1 (6.25%) of 16 samples and 3 (18.75%) samples did not react with any P primer used. G and P genotypes combination revealed that G6P5 was in 50% (8/16), G10P11 in 12.50% (2/16), G10P5 in 6.25% (1/16), G6P11 in 6.25% (1/16), G10 (P5+P11) in 6.25% (1/16), G6P? in 6.25% (1/16), G10P? in 6.25% (1/16), and G?P? in 6.25% (1/16). These results suggest that the detected genotypes can used as dominant strains for the formulation of an appropriate vaccine against BRV in Assiut Governorate. In conclusion, RT-PCR and Semi-nested multiplex PCR can used as rapid and confirmatory test for detection of nucleic acid and genotypes of Rotavirus, G and P genotypes combination in the present study revealed that G6P5, G6P11, G10P5 and G10P11 were circulating genotypes in bovine population in Assiut governorate. G6P5 strain was the most common of all strain diagnosed in other fecal samples. The presence of various combinations of G and P genotypes among field isolates of BRV suggests that genetic reassortment frequently occurred between viral strains with genes encoding different G and P genotypes. Finally, presence of different genotypes of Rotaviruses emphasizes their simultaneous monitoring in animals for the development and optimization of Rotavirus vaccines.

Background

Although the jugular vein is a major important blood vessel in equine, the literature lacks this vessel's normal B-mode and Doppler ultrasonographic examinations in donkeys. Therefore, this study aimed to determine the reference ranges of B-mode and Doppler ultrasonographic indices of jugular veins in healthy adult donkeys (Equus asinus) and the possible effect of examination side (left and right), gender, and body condition on the ultrasonographic measurements of this vessel. B-mode and Doppler ultrasound imaging of the external jugular vein was conducted on 20 adult healthy donkeys of both sexes.

Results

In all donkeys, the jugular vein was 4.01 to 8.1 mm from the body surface. The longitudinal and transverse venous diameters ranged from 3.94 to 10.5 mm and from 0.88 to 1.9 cm, respectively. Moreover, the vein areas varied from 0.61 to 2.83 cm². The reference values of superficial and deep wall thickness (SWT and DWT) were 0.56 ± 0.2 and 0.6 ± 0.13 mm, respectively. The blood velocity, blood follow rate, and congestion index of the external jugular vein can be expected in adult healthy donkeys as a range value from 8.4 to 13.5 cm/sec, from 0.33 to 1.78 ml/min, and from 0.06 to 0.27 cm.sec, respectively. Generally, the vein showed laminar monophasic waveforms. The examination side and gender have no significant effect on the ultrasound measurements of the vein (P > 0.05). Donkeys with a body condition score (BCS) ≥ 3 revealed increases in the depth of the vein (P < 0.05).

Conclusions

The results of this study can be used as reference values and provide a basis for comparison when evaluating donkeys with diseases that affect blood flow in the external jugular vein.

Broiler production is the most intensive branch of animal husbandry. This reproduction method is relatively
quick, which allows for faster turnover of the resources involved. Recent addition of Probiotics with broilers feed
shows good efficacy to improve its production. So this expermintal study aimed to examine the influence of the
addition of probiotics in broiler diets on the economic outcomes of production. Total 120 broiler chicks were divided
into three groups of fourty birds each (C, L, & H) fed with diverse concentrations of Probiotics. After the first week
of treatment, the two groups (L and H) displayed a probiotics effect that manifested as a significant increase in body
weight (BW) than the control group. After 28 days of treatment, the effect of high levels of probiotics (group H)
demonstrated a significant increase of group (H) and either group (L) and control; meanwhile, both treated groups
(L and H) exhibited a significant increase in feed intake (FI) compared with the control group. At the end of the 42-
day study period, the data showed a significant increase in BW and FI in group H compared with both group L and
the control group. Finally, the results found that the two groups L and H showed a significant increase in the most
productive performance parameters at the most periods time. The results also endly indicated that the 1 g/kg dose
(0.5 concentration) of probiotics provided for group H was better than the low concentration of probiotics provided
for group L (0.25 concentration). The study concluded that extention of the investigated probiotic (L. delbrueckii) to
broiler feed can plays an important role in improving the economic and productive efficiency of poultry farms, even
though it represents only a small part of the total or variable cost of the production of poultry.

Sepsis is a systemic inflammatory response syndrome (SIRS) that occurs when the body's immunity overreacts to an infection. It is followed by life-threatening medical consequences, including multiple organ failure (MOD). Dexmedetomidine (DEX) is a selective 2 adrenergic agonist that is used as a short-term sedative in the ICU. Apart from improving sepsis prognosis, it is believed to have an organ protecting function. Our study aimed at confirming DEX ameliorative role in sepsis-induced organ damage. We also studied DEX mitigating effect on sepsis-induced acute lung injury (ALI) and elucidated the possible mechanism. Thirty rats were randomly assigned into three groups (n=10): sham, cecal ligation and puncture (CLP)-induced sepsis, or DEX-treated CLP (DEX + CLP). 15 minutes before the CLP procedure, a prophylactic dosage of DEX (5g/kg) was given intraperitoneally (IP). Animals were slaughtered 48 hours after the surgery was completed. Histological examination for tissue samples from lung, liver and kidney. CD54 expression in lung tissue was also investigated. Blood was also taken for hematological analysis. CLP rats showed different pathological lesions in lung, kidney and liver. We reported severe pulmonary tissue damage in CLP group accompanied with enhanced CD54 expression. DEX decreased the severity of histopathological changes in the affected organs and reduced the expression of CD54 in the lung tissue as well. However, DEX could not improve sepsis-induced hematological impairment. DEX attenuated sepsis through decreased CD54 expression in the lung as well as its hepato-renal protective effect in the CLP model.

The current work was undertaken to evaluate the nephrotoxic effect of Ketoprofen on adult male rats. Eighteen rats were divided into two groups. Ketoprofen- received group (I) included 10 rats were administered Ketoprofen at a therapeutic dose of 13.5 mg/kg by I/M injection daily for 4 successive weeks. Five rats were randomly selected from group I and sacrificed at 2 and 4 weeks of the experiment. The control group (II) that received olive oil included 8 rats, where 4 rats were sacrificed after 2 weeks and the rest of rats were sacrificed after 4 weeks. Tissue specimens from kidneys of all groups were collected for histopathological examination as well as the serum was obtained for the determination of biochemical parameters. The histopathological examination of group I showed glomerular changes such as expanding of glomerular matrix, glomerular sclerosis and congestion of glomerular capillary in the cortex. Renal tubular degeneration and necrosis accompanied with infiltration of inflammatory cells in interstitial tissue in both cortex and medulla were also observed. The biochemical results revealed that animals in group I showed a significant increase in malondialdehyde, creatinine, and urea compared to the control group, while total antioxidant capacity was numerically decreased. In conclusion, the therapeutic dose of Ketoprofen caused damage in kidney tissue even if was taken for a short period as well as altered biochemical parameters.

Using an effective natural virucidal substance may be a feasible approach for preventing food-borne viral contamination. Here, the virucidal efficacy of theaflavins (TFs)-enriched tea leaf extract (TY-1) against feline calicivirus (FCV) and murine norovirus (MNV), surrogates of human norovirus (HuNoV), was evaluated. The virus solutions were mixed with various dosages of TY-1 and incubated at 25 ◦C for different contact times. TY-1 reduced the viral titer of both surrogate viruses in a time- and dosage-dependent manner. A statistically significant reduction in the viral titer of FCV by 5.0 mg/mL TY-1 and MNV by 25.0 mg/mL TY-1 was observed in 10 s and 1 min, respectively. Furthermore, TY-1 reduced the viral titer of FCV and MNV on the dry surface in 10 min. The multiple compounds in TY-1, including TFs and catechins, contributed to its overall virucidal activity. Furthermore, the effect of TY-1 on viral proteins and genome was analyzed using Western blotting, RT-PCR, and transmission electron microscopy. TY-1 was found to promote the profound disruption of virion structures, including the capsid proteins and genome. Our finding demonstrates the potential of using TY-1 as a nature-derived disinfectant in food processing facilities and healthcare settings to reduce viral load and HuNoV transmission.

The annual spread of influenza A virus (IAV) infection is a global concern. We examined the IAV-inactivating potential of theaflavin-concentrated tea extract TY-1, which contains abundant polyphenols, including concentrated theaflavins and catechins. TY-1 exhibited concentration- and time-dependent virucidal activity against IAV. Specifically, 5.0 mg/mL TY-1 induced a 1.33 and ≥ 5.17 log₁₀ 50% tissue culture infective dose/mL reduction of the viral titer compared with dextrin as the diluent control within 30 min and 6 h reaction time, respectively. The high virucidal activity of TY-1 was attributed to the combined additive activities of multiple virucidal components, including theaflavins, which led to an investigation of the virucidal mechanism of action of TY-1. Western blotting revealed that TY-1 treatment reduced the band intensity of hemagglutinin and induced the appearance of additional high molecular mass bands/ladders. In addition, TY-1 treatment also reduced the band intensity of neuraminidase (NA). A hemagglutination assay revealed that TY-1 reduced hemagglutination activity, and an NA assay revealed reduced NA activity. These results indicated that TY-1 caused structural abnormalities in IAV spike proteins, possibly leading to their destruction. Reverse transcription polymerase chain reaction (PCR) targeting the IAV genome and electron microscopic observation of viral particles revealed that upon application of TY-1, the PCR products dissipated, which indicates that TY-1 destroyed the IAV genome, and the number of viral particles reduced. Overall, TY-1 exhibited multiple modes of IAV-inactivating activity. Our findings support the possible future practical use of TY-1 as a virucidal supplemental agent that can contribute to IAV infection control.

he vagina is part of the genitalia and constitutes part of the birth canal. Sperm is deposited into vagina, and it acts in transport of sperm. Pregnancy is a complex process involving different physiological changes in the body associated with hormonal and metabolic alterations that control maternal and fetal demands. During pregnancy, neuroendocrine cells in rabbit vagina are considered part of the diffuse neuroendocrine system observed throughout the body. Giant neuroendocrine cells in rabbit vagina during pregnancy have not been observed previously. This study detected the presence of giant neuroendocrine cells in the vagina of the pregnant rabbit. The presence of these cells was demonstrated with the use of different histological techniques, including hematoxylin and eosin, PAS, combined Alcian blue-PAS, Crossmon's trichrome, and the Grimelius silver method. Giant neuroendocrine cells were observed in late-pregnancy intraepithelial sites and on the lamina propria. These cells were characterized by vacuolated basophilic cytoplasm with PAS- and PAS-AB-positive granules. Moreover, neuroendocrine cells exhibited an argyrophilic character. Immunohistochemically, neuroendocrine cells in rabbit vagina during pregnancy demonstrated positive immunoreactivity to neuron-specific enolase (NSE) with different intensities, mild immunoreactivity to the vascular endothelial growth factor (VEGF), and negative immunoreactivity to CD68.

The primo vascular system (PVS) is of great interest to scientists in the last few years. The PVS plays a role in stem cell migration, immune function, erythropoiesis, tissue regeneration, and cancer metastasis. Discovered by Bong Han Kim in 1960, the PVS was described as a new, independent system that is distributed in the body of different animals. Since then, several cells have been discovered as important constituents of the PVS. Endothelial cells are hallmarks for the identification of the PVS, as they line the sinuses of the primo vessels and are characterized by their arrangement of rod-shaped nuclei in a parallel, broken-line fashion along the primo vessels. Immune cells as mast cells, eosinophils, neutrophils, and lymphocytes are also hallmarks for the identification of the PVS. One of the most interesting cells in the PVS are p-microcells. Primo nodes are a stem cell niche due to their p-microcells content, which express stem cell biomarkers CD133 and Oct 4. Mature erythrocytes and hematopoietic stem cells have also been observed in the PVS. Hematopoietic stem cells were observed in the PVS. Hematopoietic Stem Cells are the source of the cellular component of blood. Additionally, adrenaline and noradrenaline-producing cells, mesothelial cells, and smooth-muscle-like cells have also been described in the PVS.

The current research was conducted to evaluate chemically and organoleptically some imported canned tuna sold in local supermarkets in Assiut governorate. The number of collected samples randomly was 90 canned tuna. The organoleptic assessment was done using 9- point hedonic scale ranging from dislike extremely (1) to like extremely (9). Proximate composition analysis was carried out for drained samples of canned tuna to determine moisture, crude protein, crude lipid and ash following the methodologies of the Association of Official Analytical Chemists. The results revealed that canned tuna samples contained high percentage of crude protein that ranged from 22.50% to 28.10%, with a mean value of 25.23±0.14. The moisture percentage of the examined samples varied from 61.35% to 73.55%, with a mean value of 66.24±0.26%, while total fat values varied from 0.86% to 16.68%, with a mean value of 7.32±0.40%. Ash percentage of the examined samples ranged from 0.33% to 3.18%, with a mean value of 1.47±0.07%. While total cholesterol content values of the examined samples varied from 34.62 to 51.48, with a mean value of 39.61±0.40 mg/100gm. Total carbohydrate content, caloric value, calcium content, phosphorus content, free fatty acids content (%) and fatty acid composition (%) of canned tuna samples were detected in all examined samples. From the obtained results, it is evident that all the examined canned tuna were accepted organoleptically and were considered safe for human consumption.