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Graviola Extract versus Adipose-Derived Mesenchymal Stem Cells as Therapeutics in Repairing Liver Damage Caused by 2-Amino-3-Methylimidazo [4, 5-f] quinoline

Research Abstract

The mutagenic and carcinogenic heterocyclic amine, 2-amino-3methylimidazo[4,5-f]quinoline (IQ), is produced while cooking protein-rich foods. Mesenchymalstemcells(MSCs)(ascell-basedtherapy)andAnnonamuricata(graviola) (asanaturalproduct)bothpossesspreventivecapacitiesagainst freeradical toxicity in varioustissues.ThisstudyaimstocomparethetherapeuticpropertiesandeffectsofADMSCsandgraviolaonIQ-induced liver toxicityandDNAdamage inrats. Sixtyadult maleratsweredividedintofourgroups:normalunexposedcontrol, IQ, IQ+graviola, and IQ+AD-MSCs. After 6weeks, the ratswere sacrificed, and liver tissueswere examinedforhistopathological changesusinghematoxylin−eosinstaining. p53protein expressionwasevaluatedbyimmunohistochemistry,andDNAdamagewasmeasuredby using the comet test.Our findings indicated thatAD-MSCtherapy led to themost significantimprovementinDNAdamage,apoptosis,andp53,LPO,AST,andALTlevels causedby IQtoxicity. Additionally, AD-MSCs reducedseverehistological alterations (damageandfibrosis)inlivercellsinducedbyIQ.However, theeffectivenessofgraviolatreatment islimited,severelyrestrictingits useforchroniclivertoxicity. Inconclusion, theinitial stageof IQ-inducedlivertoxicityiscausedbyoxidativestress-inducedDNA damage.Comparedwithgraviola,AD-MSCsexhibitmorepotent therapeuticeffectsagainst IQ-inducedliverdamage.

Research Authors
Doaa Hamada Abd El-Hafeez Thabet, Mona M Atia, Hanem S Abdel-Tawab, Alshaimaa AI Alghriany
Research Date
Research Department
Research Journal
ACS omega
Research Pages
53848-53864
Research Publisher
American Chemical Society
Research Vol
10
Research Website
https://doi.org/10.1021/acsomega.5c03088?urlappend=%3Fref%3DPDF&jav=VoR&rel=cite-as
Research Year
2025