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Free radicles surly attack different tissues of the body causing serious cell damage and various inflammatory conditions. As a result, there is an urgent need for discovery of new and safe antioxidants of natural sources. DPPH• radicle scavenging assay is an accurate and precise spectrophotometric method used for determination of antioxidant activity, this depends on the measurement of degree of DPPH• color change from violet to yellow spectrophoto metrically. When this technique was applied on the total and different extracts in addition to two pure flavonoids isolated from the leaves of Caryota mitis Lour., the result revealed that the plant exhibited strong antioxidant activity relative to that of standard ascorbic acid which open the field for industrial formulation of new and safe antioxidant product.

Free radicles surly attack different tissues of the body causing serious cell damage and various inflammatory conditions. As a result, there is an urgent need for discovery of new and safe antioxidants of natural sources. DPPH• radicle scavenging assay is an accurate and precise spectrophotometric method used for determination of antioxidant activity, this depends on the measurement of degree of DPPH• color change from violet to yellow spectrophoto metrically. When this technique was applied on the total and different extracts in addition to two pure flavonoids isolated from the leaves of Caryota mitis Lour., the result revealed that the plant exhibited strong antioxidant activity relative to that of standard ascorbic acid which open the field for industrial formulation of new and safe antioxidant product.

Free radicles surly attack different tissues of the body causing serious cell damage and various inflammatory conditions. As a result, there is an urgent need for discovery of new and safe antioxidants of natural sources. DPPH• radicle scavenging assay is an accurate and precise spectrophotometric method used for determination of antioxidant activity, this depends on the measurement of degree of DPPH• color change from violet to yellow spectrophoto metrically. When this technique was applied on the total and different extracts in addition to two pure flavonoids isolated from the leaves of Caryota mitis Lour., the result revealed that the plant exhibited strong antioxidant activity relative to that of standard ascorbic acid which open the field for industrial formulation of new and safe antioxidant product.

Phytochemical analysis and chromatographic fractionation of the total ethanolic extract of Caryota mitis Lour. resulted in isolation and identification of ten compounds, two of them are firstly reported in the family Arecaceae; kaempferol-3-O-rutinoside (8) and chlorogenic acid methyl ester (10), six compounds are firstly reported in the plant; β-amyrin (1), β-sitosterol (2), β-sitosterol-3-O-β-D glucoside (3), kaempferol (4), quercetin (5), kaempferol-3-O-β-D-glucopyranoside (6) and two compounds previously isolated from the plant; quercetin-3-O-β-D-glucopyranoside (7), quercetin-3-O-rutinoside (9). The structures were identified and confirmed through different spectroscopic methods including 1H-NMR, 13C-NMR, EI-MS and UV spectroscopy, in addition to comparison with authentic samples. Antimicrobial assay of the different extracts and fractions revealed strong antibacterial activities on Staph. aureus more than E. coli; in addition to moderate antifungal activity of n-butanol and aqueous fractions against Candida albicans.

Phytochemical analysis and chromatographic fractionation of the total ethanolic extract of Caryota mitis Lour. resulted in isolation and identification of ten compounds, two of them are firstly reported in the family Arecaceae; kaempferol-3-O-rutinoside (8) and chlorogenic acid methyl ester (10), six compounds are firstly reported in the plant; β-amyrin (1), β-sitosterol (2), β-sitosterol-3-O-β-D glucoside (3), kaempferol (4), quercetin (5), kaempferol-3-O-β-D-glucopyranoside (6) and two compounds previously isolated from the plant; quercetin-3-O-β-D-glucopyranoside (7), quercetin-3-O-rutinoside (9). The structures were identified and confirmed through different spectroscopic methods including 1H-NMR, 13C-NMR, EI-MS and UV spectroscopy, in addition to comparison with authentic samples. Antimicrobial assay of the different extracts and fractions revealed strong antibacterial activities on Staph. aureus more than E. coli; in addition to moderate antifungal activity of n-butanol and aqueous fractions against Candida albicans.

Phytochemical analysis and chromatographic fractionation of the total ethanolic extract of Caryota mitis Lour. resulted in isolation and identification of ten compounds, two of them are firstly reported in the family Arecaceae; kaempferol-3-O-rutinoside (8) and chlorogenic acid methyl ester (10), six compounds are firstly reported in the plant; β-amyrin (1), β-sitosterol (2), β-sitosterol-3-O-β-D glucoside (3), kaempferol (4), quercetin (5), kaempferol-3-O-β-D-glucopyranoside (6) and two compounds previously isolated from the plant; quercetin-3-O-β-D-glucopyranoside (7), quercetin-3-O-rutinoside (9). The structures were identified and confirmed through different spectroscopic methods including 1H-NMR, 13C-NMR, EI-MS and UV spectroscopy, in addition to comparison with authentic samples. Antimicrobial assay of the different extracts and fractions revealed strong antibacterial activities on Staph. aureus more than E. coli; in addition to moderate antifungal activity of n-butanol and aqueous fractions against Candida albicans.

Caryota mitis Lour. palm belongs to Family: Arecaceae. Pyridine and piperidine alkaloids are the most bioactive secondary metabolites reported in this Family. The dried leaves of Caryota mitis Lour. were subjected to defatting using n-hexane. The alkaloids were extracted from the defatted powder (marc) by acid base method. Eight pyridine/ piperidine alkaloids were detected by LC-MS and reported for the first time in the genus Caryota. The alkaloid fraction as well as other plant extract fractions showed antibacterial and antifungal activities (MIC) against selected strains.