Our research aimed to evaluate the difference in water sorption, water solubility and push out bond strength between three bioceramic sealers (CeraSeal, NeoSEALER Flo, Well Root ST) with (AH plus).

Materials and methods

For water sorption and solubility, we prepared thirty-two discs and incubated until complete setting. According to the type of sealer, they were divided into four groups (n=8). The percentage of water sorption was calculated utilizing this formula: percentage of water sorption= (weight gained-initial weight /initial weight) x100. Percentage of water solubility = (water solubility / Dry weight) x100. For push out bond strength, instrumentation and irrigation were performed for forty single rooted teeth. Four groups were created according to sealer used. Cross-sectional cuts were made perpendicular to the root’s long axis for each specimen. The peak load observed was divided by the surface area to get the bond strength using the formula (A=(3.14 h (r1+r2)).

Results

For solubility, Ah plus showed lower solubility after 1 and 3 days but Ceraseal showed lowest solubility after 7 days. For water sorption, Neosealer Flo showed lowest water sorption after 1 day but AH plus showed lowest water sorption after 3 and 7 days. For push out bond strength, for coronal section, Ah Plus and Well-Root ST showed a significant highest push out bond strength in comparison with Ceraseal and Neosealer Flo.

Conclusion

The bioceramic sealers had high water solubility and water sorption which allowed the release of biologically relevant ions. All the sealers bond strength meets the required standards.

The aim of this study was to assess and draw comparisons between the antibacterial properties of various bioceramic sealers.

Materials and methods

The samples were categorized according to the sealer type that was utilized. Group 1: Well Root ST, group 2: Ceraseal, group 3: Neosealer Flo, group 4: AH plus. Manufacturer's instructions were followed when preparing the sealers and placed in sterile plastic cylinders with a diameter and depth of 5 mm. Following that, specimens were incubated at 37°C and 100% humidity for 7 days. The bacterial suspension was made with a standardized density of 0.5 on the MacFarland (1.5x108/ml). The suspensions were diluted 10,000 times after mixing for six, fifteen, and sixty minutes, and then a triplicate amount of 0.01 from each diluted suspension was placed onto the pre-prepared BHI agar plates. (Difco Lab., Detroit, MI, USA). The number of colonies forming unit for each sealer was calculated in different time throughout the experiment.

Results

For the effect on Enterococcus faecalis at 6 minutes, Neosealer Flo showed lowest bacterial count, at 15 minutes AH plus showed lowest bacterial count and 60 minutes Ceraseal showed lowest bacterial count. For the streptococcus mutans at 6 minutes, Neosealer flo showed lowest bacterial count, at 15 min AH plus and Well root ST showed lowest bacterial count and at 60 min well Root ST showed lowest bacterial count.

Conclusion

All sealers showed antibacterial effect, where increasing the time resulted in decreasing Log10 CFU.

This study seeks to assess the cytotoxic impact of various bioceramic sealers, namely Well-Root ST, CeraSeal, and NeoSEALER Flo, in comparison to AH Plus sealer. The evaluation encompasses both the fresh and set states of these sealers, employing the MTT assay on human periodontal ligament fibroblasts.

Materials and methods

Following the manufacturers' instructions, the four tested sealers were blended in a sterile environment and then placed into standardized plastic rings. Extracts derived from the tested sealers were applied to human periodontal ligament fibroblasts. The freshly mixed sealers were examined immediately after mixing, with their extracts prepared at that moment. On the other hand, the remaining specimens, designated as set specimens, were incubated in a humidified environment with 5% CO2 and 95% air at 37º C for 24 hours before extraction to create extracts of the tested sealers. The extracted material was then diluted with DMEM to achieve twelve distinct concentrations of each extract 50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78%, 0.4%, 0.2%, 0.1%, 0.05%, and 0.025%. The cytotoxicity of all root canal sealers was evaluated using the MTT assay, followed by the calculation of cell viability percentages.

Results

AH Plus showed the highest toxicity followed by the NeoSEALER Flo then Well-Root ST and the least toxicity was CeraSeal.

Conclusion

The assessed root canal sealers exhibited differing levels of cytotoxicity, and the rise in cell viability percentages was contingent on the concentration.