Background Regarding their distinct physico-chemical and bioactivity characteristics, silver nanoparticles ‘AgNPs’ are
extensively utilized in numerous scientifc purposes.
Results Within this current investigation, for the frst time, we evaluated how the extracellular extract of the
isolate MAK223 generated exceptionally fxed AgNPs. The isolate was genetically identifed as Aspergillus templicola
OR480102. The generated AgNPs’ physico-chemical characteristics were assessed using ultraviolet-vis spectroscopy,
transmission electron microscopy (TEM), and Fourier transform infrared spectrometry (FT-IR). The maximum
absorption in the UV-vis spectrum was obtained at 420 nm, matching the silver nanoparticles’ surface plasmon
absorbance. A. templicola OR480102 produced uniformly dispersed AgNPs between 5 and 25 nm with a mean
dimension of 17.78537 ± 1.36 nm using TEM. FT-IR analysis identifed functional groups (e.g., -OH, C = O) in the
fungal fltrate that mediate AgNP synthesis and capping. To verify AgNPs stability, the dynamic light scattering
(DLS) approach is employed. Optimal conditions for AgNPs synthesis were 10 days of incubation, one mM silver
nitrate concentration, pH 11, and elevated temperatures. AgNPs demonstrated efcacy against clinically relevant
pathogens: S. typhimurium ‘ATCC 14028’, B. subtilis ‘ATCC 6633’, S. aureus ‘ATCC 25923’, and E. coli ‘ATCC 29213’ were
used in the study. Also, using AgNPs derived from the fltrate of A. templicola OR480102 shows signifcant potential
as a novel therapeutic approach against breast cancer cells ‘MCF-7’. The scratch assay of ‘MCF-7’ cells demonstrates
the suppressive impact of AgNPs for these cell lines during proliferation by promoting apoptosis and reducing cell
migration.
Conclusion Based on physico-chemical characteristics of AgNPs’ and their antimicrobial and anticancer activities,
it cleared that the selected strain Aspergillus templicola OR480102 is a promising producer of stable AgNPs’ with
signifcant bioactivities which could be applicable in different felds.