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Evaluation of bromadiolone combined with ciprofloxacin, vitamin D, aspirin, and cinnamon as an apoptosis-mediated rodenticide strategy

Research Abstract

This manuscript explores whether co-formulation of the second-generation anticoagulant bromadiolone with additives such as ciprofloxacin, vitamin D, aspirin, and cinnamon can enhance rodenticidal efficacy at reduced doses, while assessing hepatic pathomorphology, oxidative stress, coagulation, DNA damage, and apoptosis in wild rats. The study is both timely and relevant, addressing ecological and public health concerns by investigating mechanistic pathways including the p53–p21–caspase axis, lipid peroxidation, comet assay, prothrombin time, and serum enzyme levels. A key strength lies in its novel strategy of combining bromadiolone with pharmacological and food-derived compounds, offering practical implications for minimizing environmental impact. The multidimensional dataset spanning biochemical, molecular, genotoxic, coagulation, and histopathological endpoints provides strong mechanistic depth. Findings suggest that certain additives, particularly cinnamon and aspirin, potentiate oxidative stress and apoptosis, correlating with increased mortality and liver damage. Overall, the integration with existing literature on vitamin K antagonism, cholecalciferol-induced hypercalcemia, NSAID-mediated apoptosis, and coumarin derivatives highlights the study’s mechanistic grounding and translational relevance.

Research Authors
Heba Allah Ahmed Mahmoud Ahmed, Magdy Wilson, Alshaimaa AI Alghriany, Mona M Atia
Research Date
Research Department
Research File
Research Journal
Scientific Reports
Research Publisher
Nature Publishing Group UK
Research Vol
15
Research Website
https://doi.org/10.1038/s41598-025-28468-7
Research Year
2025