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Occurrence of xanthomonas axonopodis pv. phaseoli, the causal agent of common bacterial blight disease, on seeds of common bean (Phaseolus vulgaris L.) in upper Egypt

Research Abstract
Common bean seed lots collected from different seed dealers and Malawii agriculture station were screened for the presence of Xanthomonas axonopodis pv . phaseoli . In the laboratory the pathogen was isolated following the routine laboratory assay method, i.e . direct plating method using yeast extract–dextr- ose–calcium carbonate agar medium (YDC). Yellow, convex, mucoid colonies of Xanthomonas were con- sistently isolated on YDC from seed samples. The presumptive pathogen was confirmed by isolation on semiselective medium, such as mTBM and MD5A. Further, the pathogen was confirmed by biochemical, physiological and, finally, the pathogenicity test s. Five samples out of seven were positive for Xanthomonas . The isolates were found to cause common blight of 3-week-old common bean plants by 7 d after inoculation. Bacteria with the same characteristics as those inoculated were re-isolated from the infected plants.
Research Authors

MH Abd-Alla, SR Bashandy, S Schnell
Research Journal
Folia microbiologica
Research Member
Research Pages
47-52,
Research Rank
1
Research Vol
55 (1)
Research Year
2010