The quantitative and qualitative output of numerous economic crops around the globe is severely limited by
plant-parasitic nematodes (PPNs). The use of synthetic nematicides remarkably causes environmental pollution;
the best alternative for pollution prevention is organic inputs. This study focused on testing nine types of carrots
to determine which carrots are resistant to and susceptible to Meloidogyne javanica. Five weed species were
studied for their effects on Meloidogyne javanica egg masses and second-stage juveniles (J2s). These include
Dipsacus sylvestris (Ds), Euphorbia prostrata (Ep), Malvastrum tricuspidatum (Mt), Lepidium didymum (Ld), Portulaca
oleracea (Po) and Sonchus asper (Sa). In vitro-tested weeds were further utilized against M. javanica in management
(in vivo) experiments with highly susceptible cultivars. The gall index of carrot roots revealed that the
cultivar Golden Rosy was resistant, whereas the cultivar Red Core was highly susceptible to the nematode. In
vitro tests revealed that extracts of certain weeds effectively stopped the second-stage juveniles of M. javanica.
However, only bioactive compounds released from Ld and Sa caused the highest mortality rates (86.8 % and
82.4 %), with LC50 values of 0.016 % and 0.022 %, respectively, and the highest egg hatching inhibition rates
(80.0 % and 78.4 %, respectively) of J2s from M. javanica at a 100 % concentration. This study also used virtual
screening to explore how the chemicals from Meloidogyne javanica, Lemna duckweed (Ld), and Spirodela polyrhiza
(Sa) interact with each other. We used a homology-built receptor protein model for molecular docking. The
phytocompound 4,4-dimethyl-androst-5-ene-3-ol had the best af􀁀nity for binding to key amino acid residues,
with a docking score of 7.5 kcal/mol. The lowest binding af􀁀nity, 􀀀3.9 kcal/mol, was found for 1,8,11-heptadecatriene
(Z,Z) when it contacted the receptor. Among the 33 compounds found in the plants that were tested, 4,4-
dimethyl-androst-5-ene-3-ol and stigmast-5-ene-3-ol were the most effective at preventing M. javanica from
growing in multiple models. The use of leaf powder (5 g) of Ld along with chopped leaves of Ds, Ep, Mt, Po, and
Sa weeds (30 g each) on M. javanica in vivo revealed that all of the treatments had a signi􀁀cant nematicidal effect
on the nematode population, although to different degrees, and helped the carrot plants grow. These 􀁀ndings
support the potential of the above weeds as nematode control agents, suggesting their viability over synthetic
nematicides. This study can help with long-term nematode control plans where phytocompounds, mostly
stigmast-5-ene-3-ol and 4,4-dimethyl-androst-5-ene-3-ol, are used as eco-friendly bionematicides.