Investigation of an alternative source of the nonrenewable energy is attracting the world attention nowadays. The fruits and leaves biomass of Calotropis procera were exposed to sequential pretreatment processes. First, a successive pretreatment with an organic solvent (hexane/methanol) yielded biocrude and plant spent residues fractions. The plant spent residues fractions were exposed to successive acid/alkali (1% H₂SO₄/2% NaOH) pretreatment to yield spent residues of fruits (SRF) and leaves (SRL). The amount of cellulose content of the sequential pretreated SRF and SRL were 87.3 and 83.4%, respectively. The hydrolysis process of 10 g of the sequential pretreated SRF and SRL by Sternzym cellulase produced 80.2 and 50.4 g/L of total reduced sugars, respectively. The reduced sugars were then fermented by Saccharomyces cerevisiae (MN901244) for bioethanol production. The HPLC analysis showed 38.9 and 23.8 g/L of bioethanol yields from sequential pretreated SRF and SRL, respectively. The GC/MS analysis of methanol and hexane biocrude fractions revealed the presence of 20 and 12% of fatty acids, respectively, which would be promising amounts for biodiesel production after esterification. So, the present study suggested a low-cost nonedible plant biomass (Calotropis procera) as a biorefinery for production of renewable bioethanol.