A total of 506 isolates of mesophilic, thermophilic and thermotolerant fungi isolated from the poultry feed ingredients included soybean meals, ground maize, cottonseed cake, wheat bran and fish meal, on glucose‐CZAPEK's agar, LITTMAN oxgall agar at 28 °C and yeast starch agar (YPSs) at 45 °C, were screened for their ability to produce hydrolytic protease enzyme on solid media. Most of the fungal isolates were able to produce such enzymes but with variable capabilities. The highest proteolytic activity was exhibited by some isolates of Penicillium chrysogenum, Aspergillus flavus, Thermoascus thermophilus and Rhizopus chizopodifarmis. Of all fungal isolates screened for proteolytic activity, Penicillium chrysogenum and Thermoascus thermophilus produced the highest amounts of proteases. These two isolates were used to study the effect of some environmental and nutritional factors on their proteolytic …

CiNii 論文 - Bradykinin potentiating fraction isolated from venom of Buthus occitanus promotes spermatogenesis in premature mice CiNii 国立情報学研究所 学術情報ナビゲータ[サイニィ ] 日本の論文をさがす 大学図書館の本をさがす 日本の博士論文をさがす 新規登録 ログイン English 検索 すべて 本文あり すべて 本文あり 閉じる タイトル 著者名 著者ID 著者所属 刊行物名 ISSN 巻号 ページ 出版者 参考文献 出版年 年から 年まで 検索 検索 検索 [10/4更新]2022年4月1日からの CiNii ArticlesのCiNii Researchへの統合について Bradykinin potentiating fraction isolated from venom of Buthus occitanus promotes spermatogenesis in premature mice NASSAR AY 被引用 文献: 1件 著者 NASSAR AY 収録刊行物 Rec. Advan. Toxinol. Res. Rec. Advan. Toxinol. Res. 2, 119-135, 1992 被引用文献: 1件中 1-1件を 表示 1 A Scorpion Venom Peptide Fraction Induced Prostaglandin Biosynthesis in Guinea Pig Kidneys : Incorporation of ^<14>C-Linoleic …

The study examines the anti-ulcer activity of Cu(I)-(nicotinic acid)₂Cl [CuCl(HNA)₂]. A dose of 8 mg (23 μmol) of complex/kg body mass was suspended in 0.25% Tween-80 in saline solution and administered intragastrically to male Wistar albino rats which had developed gastric ulcers as a result of pyloric ligation (Shay-rat model). Another group of animals received 5 mg (25 μmol)/kg body mass of the copper-glycinate complex Cu(II)(glycinate)₂ [Cu(II)(Gly)₂]. Both protected as shown by reduction in the ulcer index, inhibition of gastric perforation and death. Significant increases in gastric juice volume and superoxide dismutase (SOD) activity in the gastric mucosa and blood plasma were found with both copper complexes, while the gastric juice prostaglandin E₂ (PGE₂) content was significantly decreased in the Cu(II)(Gly)₂-treated group, it was significantly increased in the gastric mucosa of the CuCl(HNA)₂-treated …

[en] The buthus occitanus, scorpion venom contains a strong bradykinin potentiating factor (BPF) that augments bradykinin effects through enhancing its release. Based on the cytoprotective ability of BPF, the present work investigates it as a radioprotectant. Sublethal whole-body y-irradiation at 1.5 Gy was used. Bone marrow cells suspension (BM cells) alone or in combination with BPF was utilized. Three to four weeks-aged male Guinea pigs were grouped into two major groups. The first was non-irradiated control that was divided into subgroups treated ip with BM cells (2.5 xl06 cells), one dose of BPF (lug/gb wt), BM cells+ BPF, one week spaced two doses of BPF, BM cells+ 2 doses of BPF, one week spaced three doses of BPF or BM cells+ 3 doses of BPF. Second major group (irradiated group) at 1.5 Gy that, subdivided and treated similarly. 5 animals from each group were killed at 7, 14 and 21 days from the initiation of treatment (3 h after irradiation). The subgroups of non-irradiated animals showed an increase in spleen wt and colony formation, thymus population, and globulins content particularly in those subgroups that stayed for the later time periods (14 and 21 days) and that treated with combined BM cells+ BPF or that groups that were treated with two or three BPF doses. Irradiation caused dramatic destruction in thymus and the spleen reflected on reduction of the lower globulins content. Treatment with BM cells, BM cells+ double doses of BPF or triple doses of BPF caused complete recovery in all measured indices, the best result was observed in those of subgroups treated with BM cells+ double doses of BPF or treated with triple …

ABSTRACT
Cisplatin (CP) is considered as a major antineoplastic drug against a broad spectrum of malignancies. CP acts on cancer cells by releasing free radicals which at the same time damage liver and kidney cells. The tissue specific toxicity of cisplatin to the kidneys is well documented. However, at higher doses less common toxic effects such as hepatotoxicity may arise. Strategies to protect tissues against CP toxicity are of clinical interest. In this study, we aimed to investigate the hepatotoxicity of CP which it may be mediated by oxidative stress and to establish whether some antioxidants, namely vitamin C, N,NDiphenyl- X-phenylenediamine (DPPD) and L-cystiene, may provide protection against CP hepatotoxicity. Forty adult male albino rats (1 20-1 50 g) were divided into 5 groups (8 rats each). CP was injected once a week (2 mg/kg) for four weeks. The antioxidants DPPD (1 25 mg/kg b.w. ), vitamin C (1 00 mg/kg) and L-cystiene (1 00 mg/kg) were also injected once a week 24 hour prior to CP injection. The control group
was injected with saline. All doses were injected intraperitoneally. Rats of different groups were killed by cervical dislocation, 24 hours after the last injection and blood was collected into a sterilized tube containing EDTA to separate plasma. The livers was taken for histological and biochemical examinations. CP-induced oxidative stress was indicated by increased level of LPO and superoxide anion in hepatic tissue and plasma. Also, CP induced decline of antioxidant enzymes such as SOD, CAT, GST and GGT and a decreased level of GSH, Vit. C and Vit. E in hepatic tissue and plasma. Treatment with Vit. C, DPPD and L-cysteine in combination with CP restored LPO and superoxide anion,
the activities of SOD, GST, CAT and GGT and the content of GSH, Vit. C and Vit. E to about normal control levels. in conclusion treatment with Vit. C, DPPD or L-cysteine in combination with CP may be effective to protect from oxidative hepatic injury that induced by CP treatment.

The present study was planned to investigate the effect of dietary supplementation with copper Chelating complex as immunstimulant of catfish Claris gariepinus. Forty acclimated African sharp tooth catfish, were divided into two groups. The first group (control group G I) received the basic diet, for 30 days. The second group received the basal diet mixed with 60 mg copper albumin chelating complex/kg basal diet; (G II). At the end of 30 days feeding, the fish of each group were, weight, blood film and serum sample were collected and tested. Fish of each group were challenged by local strain of Aeromonas hydrophila, for challenge. The clinical signs, P.M. lesions and mortalities were also monitored for 7 days post challenge. The obtained results were indicated that copper chelating complex improve the growth rate, and enhance the protective effect against challenge with A. hydrophila and potentiate the non-specific immune response through increase the percent of basophile, the lymphocytes and increase the total protein and the globulin in the blood of the treated fish. In conclusion use of copper chelating complex as feed additive for Clariasgariepinus at a dose of 60mg / kg diet has shown to be an activator of non-specificimmune response.

In a previous work we have reported that synthetic copper (I)-nicotinate complex efficiently prevented induced nephrotoxicity by Aflatoxin B1 (AFB1) specifically by promoting phase II detoxificating glutathioneS-transferase activity. This work has been conducted to evaluate the antitoxic effect of the complex on the metabolism of AFB1. Forty five healthy male Wister albino rats were intoxicated (20 µg/kg B.W.) day after day for five weeks. The individually collected urine was assayed for AFB1, AFM1 and AFQ1 contents by HPLC technique. One third of them were co-treated by butylated hdroxytoluene (BHT) (0.05 g/kg B.W.), the second was co-treated by the copper complex (400 µg/kg B.W.) while the last was only intoxicated untreated group. Significant increase in the less toxic AFM1 and AFQ1 metabolites was recorded by any of the co-treating agents. The output of the least toxic AFQ1 fraction was significantly increased by the copper complex as regard to the BHT, (P <0.05). Conclusively, AFM1 and AFQ1 fractions in the urine that were promoted by the two tested agents may be related to their potentiating activity upon the phase I detoxificating CYP 1A2 and 3A4 enzyme families respectively. The significantly increased detoxified AFQ1 metabolite may be preferentially promoted by the copper (I)-nicotinate complex. This beneficial detoxificating effect of the copper complex has been obviously confirmed by observed great reduction in the immunohistochemical detection of vascular endothelial growth factor that was highly initiated by aflatoxicosis. Hence, such a complex could be saftly accepted as a highly potent antitoxic agent against aflatoxicosis