Purpose This study was carried out to assess the prevalence of Trypanosoma evansi infection in naturally diseased Dromedary camels in Dammam, Eastern region of Saudi Arabia. The detection of Trypanosoma evansi was performed using the parasitological, serological, and molecular diagnosis and a comparison between such methods were analyzed. In addition, an evaluation of the therapeutic efficacy of selected antitrypanosomal drugs, cymelarsan and quinapyrmine (aquin-1.5), was trialed for the treatment of diagnosed infected cases.
Methods A total 350 randomly selected camels were evaluated using thin blood smear (TBS), RoTat1.2 PCR and CATT/T. evansi techniques.
Results The total prevalence was 6.9%, 7.7%, and 32.8% by TBS, RoTat1.2 PCR and CATT/T. evansi techniques, respectively. Although PCR detect T. evansi in more samples than TBS, the agreement was good (K = 0.9). Among the CATT/T. evansi results, PCR detected T. evansi in 12 and 15 CATT positive and negative camels, respectively, with the low agreement (Kappa = 0.1). The use of cymelarsan and quinapyramine sulfate in the treatment of naturally infected cases demonstrated a very efficient therapeutic response.
Conclusion It was found that
1. Comparing the CATT/T. evansi and PCR results, the
positivity of CATT was higher than PCR detection, while the agreement was poor (K = 0.1).
2. Cymelarsan and aquin-1.5 proved to be effective in the treatment of naturally infected camels, but cymelarsan presented with higher effectiveness (100%) than aquintreated camels (83.3%). a
3. The use of cymelarsan and CATT is recommended for disease treatment and control.
Keywords Trypanosoma evansi · RoTat 1.2VSG PCR · CATT/T. evansi · Cymelarsan
 

Background: Sudden death is defined as an unexpected death occurring with no observed antecedent clinical signs.
Aim: The current study was performed to notice the tangible causes of sudden death among 51 out of 340 she-camels on a private farm in the eastern region of El Khafgi, Saudi Arabia.
Methods: A retrospective cohort study design was conducted to investigate the sudden death of camels through
microscopic examination of fecal matter to identify the gastrointestinal parasites, analysis of whole blood thin films to diagnose blood parasites, blood culturing to recognize bacterial infection as Pasteurella multicida, and macroscopic postmortem examination to identify the gastrointestinal adult worm. The quantity and composition of feed were also analyzed. Afterward, a commercial multiscreen Ag-ELISA kit technique determined the toxins of Clostridium perfringens (C. perfringens).
Results: The results revealed that the incidence rate of sudden death was 15%. The sudden death occurred due to C. perfringens enterotoxins detected in the rumen, intestinal content, and intestinal wall. The enterotoxins and Alpha toxins were noticed, but the other toxin types, including Beta and Epsilon, could not be detected. All C. perfringens toxins were discovered to be negative in fecal matter. A significant association was reported between sudden death, she-camel age, and feeding habits as risk factors (p = 0.020 and 0.028, respectively). Risk factor assessment by relative risk (RR) revealed that the odds of RR of sudden death occurring among she-camels over two years were higher than those under two years (2.24 CI 95%, 1.093–4.591). Furthermore, the odds RR of sudden death occurring due to exposure of she-camels to a concentrated ration of 18% were higher twice than those not exposed (2.346 CI 95%, 1.039–5.296).
Conclusion: Clostridium perfringens enterotoxaemia should be listed as a cause of sudden death in camels. The alteration in diet with 18% concentration feed changes the intestinal environment, leading to C. perfringens proliferating and yielding potent toxins. More observations and interferences like regular immunization are recommended to reduce the disease and increase the farmers' awareness of the importance of risk factors.
Keywords: Camels, Clostridium perfringens, Enterotoxins, Risk factors, Sudden death.
 

The bulbus arteriosus of goldfish, Carassius auratus, possesses unique structural features. The wall of the bulbus arteriosus is exceptionally thick, with an inner surface characterized by longitudinally arranged finger-like ridges, resulting in an uneven luminal appearance. These ridges are covered by endocardium and encased in an amorphous extracellular matrix. The inner surface of the bulbus arteriosus also contains rodlet cells at different developmental stages, often clustered beneath the endothelium lining the bulbar lumen. Ruptured rodlet cells release their contents via a holocrine secretion process. The high abundance of rodlet cells in the bulbus arteriosus suggests that this is the site of origin for these cells. Within the middle layer of the bulbus arteriosus, smooth muscle cells, branched telocytes (TCs), and collagen bundles coexist. TCs and their telopodes form complex connections within a dense collagen matrix, extending to rodlet cells and macrophages. Moreover, the endothelium makes direct contact with telopodes. The endocardial cells within the bulbus arteriosus display irregular, stellate shapes and numerous cell processes that establish direct contact with TCs. TEM reveals that they contain moderately dense bodies and membrane-bound vacuoles, suggesting a secretory activity. TCs exhibit robust secretory activity, evident from their telopodes containing numerous secretory vesicles. Furthermore, TCs release excretory vesicles containing bioactive molecules into the extracellular matrix, which strengthens evidence for telocytes as promising candidates for cellular therapies and regeneration in various heart pathologies.

Fish's skin serves a variety of functions that are essential for survival, including communication, respiration, sensory perception, excretion, ion regulation, and heat regulation. This research aimed to examine the snout skin of koi fish (Cyprinus carpio) to determine its structural characteristics. Using light microscopy, the histochemical elements of the skin were examined in a total of 20 adult, healthy koi fish. The skin of was made up of three layers: epidermis, dermis, and hypodermis. The epidermis consisted of epidermal cells, club cells, mucous goblet cells, rodlet cells, eosinophilic granular cells, serous goblet cells, and melanocytes. large amounts mucous of cells which reacted positive to Alcian blue (AB) and Periodic Acid–Schiff (PAS), few club cells which reacted positive to bromophenol blue and light green. many eosinophilic granular cells (EGCs) reacted positive to PAS and light green. Moreover, many taste …

The aim of this study was to detect illegal adulteration of beef meat products with meat from other species. Samples (n= 120) of industrial and handmade beef products were randomly collected from retail outlets in Assiut city, Egypt: raw beef burger, oriental beef sausage, beef kofta, and beef luncheon (30 samples each). Samples were analysed using agar gel immunodiffusion (AGID) and modified AGID (MAGID) assays. Results showed that 17.5% of examined products were adulterated with chicken meat. MAGID detected that 14.1% of samples were adulterated with donkey meat, whereas all AGID results were negative. Human tissue was detected in 8.3%(AGID) and 10%(MAGID) of examined samples. Histological examination was then used to detect foreign tissue, and all categories of products were found to be adulterated, and some of them-contaminated with human blood cells. Polymerase chain reaction analysis confirmed that MAGID was more accurate and sensitive than AGID, especially for false negative AGID results. Consumers are advised not to consume too much of the studied meat products to avoid exposure to adulterated or contaminated products that might constitute a health hazard.

Adult cartilage comes in three different types: hyaline cartilage, elastic cartilage, and fibrocartilage. In several forms of cartilage, chondrocytes are described as a one-cell population. Chondrocytes are the manufacturers of the surrounding ECM and collagen type II fibers in hyaline cartilage besides the elastic fibers in elastic cartilage. Whereas the white adipocytes mainly compose the white adipose tissue and they are specialized in production, storage and mobilization of triglycerides. Early studies explored a unique type of chondrocyte in mouse, rat, and rabbit auricular cartilage having morphology similar to white adipocytes and identified it as "adipochondrocyte". The objective of the current study was to explore the differences between chondrocyte, adipocyte and adipochondrocyte morphologies in white New Zealand rabbits and to ascertain if adipochondrocyte is more comparable to chondrocyte or adipocyte morphology. The auricles, articular cartilage of carpal joint, and pre-renal white fat of adult male white rabbits were harvested and processed for histological examination with light and transmission electron microscopy. The adipochondrocytes appeared as hypertrophic white adipocyte-like chondrocytes occupied the auricular cartilage plate of the white New Zealand rabbits, similar to the characteristic "signet ring" appearance of the white adipocytes in pre-renal white fat. The adipochondrocytes were housed in lacunae within an ECM similar to chondrocytes of articular cartilage. The TEM examination had illuminated that the adipochondrocyte cytoplasm contained large lipid globule that flattened the eccentric nucleus and sparse …

Background The liver was identified as a primary target organ for the chemo-radiological effects of uranyl acetate (UA). Although the anti-oxidant and anti-apoptotic properties of gallic acid (GA) make it a promising phytochemical to resist its hazards, there is no available data in this area of research.

Methods To address this issue, eighteen rats were randomly and equally divided into three groups. One group was received carboxymethyl cellulose (vehicle of GA) and kept as a control. The UA group was injected intraperitoneally with UA at a single dose of 5 mg/kg body weight. The third group (GA+UA group) was treated with GA orally at a dose of 100 mg/kg body weight for 14 days before UA exposure. UA was injected on the 15th day of the experiment in either the UA group or the GA+UA group. The biochemical, histological, and immunohistochemical findings in the GA+UA group were compared to both control and UA groups.

Results The results showed that UA exposure led to a range of adverse effects. These included elevated plasma levels of aspartate aminotransferase, lactate dehydrogenase, total protein, globulin, glucose, total cholesterol, triglycerides, low-density lipoprotein cholesterol, and very-low-density lipoprotein and decreased plasma levels of high-density lipoprotein cholesterol. The exposure also disrupted the redox balance, evident through decreased plasma total antioxidant capacity and hepatic nitric oxide, superoxide dismutase, reduced glutathione, glutathione-S-transferase, glutathione reductase, and glutathione peroxidase and increased hepatic oxidized glutathione and malondialdehyde. Plasma levels of albumin and alanine aminotransferase did not significantly change in all groups. Histopathological analysis revealed damage to liver tissue, characterized by deteriorations in tissue structure, excessive collagen accumulation, and depletion of glycogen. Furthermore, UA exposure up-regulated the immuno-expression of cleaved caspase-3 and down-regulated the immuno-expression of nuclear factor-erythroid-2-related factor 2 in hepatic tissues, indicating an induction of apoptosis and oxidative stress response. However, the pre-treatment with GA proved to be effective in mitigating these negative effects induced by UA exposure, except for the disturbances in the lipid profile.