A detailed Clinico-pathological profile of clinically diseased cattle and buffaloes with Theileriosis located in Al-Ghaniem region, Assiut Governorate, was aimed. Theileria annulata was confirmed by the presence of T. annulata piroplasms in blood smears and/or lymph smears followed by polymerase chain reaction (PCR). During the period of investigation (April 2015 to August 2018), out of the clinically inspected cattle (n= 300) and buffaloes (n= 100), 80 (26.67%) and 15 (15 %) cases were clinically suspected to have Theileriosis, respectively. The positive cases were molecularly identified (PCR). The general observed signs were anorexia, fever, swelling of superficial lymph nodes. Ocular lesions were white cloudiness were more obvious in the center of cornea rather than the borders (yellowish colored corneoscleral opacity surrounded by hyperemic band). A watery discharge from the eyes. Serous ocular discharge (watery lacrimation) was remarkable, however in severe cases the ocular discharges was accumulated in the medial canthus. Some newly born calves of less than one month exposed to ocular symptoms mainly protruding of eye ball with ictric conjunctiva. The clinical examination of conjunctivae of the clinically suspected cases with Theileriosis indicated that icteric appearance of conjunctivae in some cases. Three cases showed petechiated conjunctivae. In our study some animals showed up--word visible bulging of temporal fossa. Visible protrusion of hemorrhagic conjunctiva with apparently exophthalmia (ocular edema) were observed. Bloody diarrhea and tarry like diarrhea, change in feeding behavior or habit like depraved appetite by eating mud ,soil were noticed. On the other side, the most prominent necropsy features the recently succumbed animals: Gross changes in various organs including heart lungs, trachea, stomach, liver, spleen, kidneys superficial lymph nodes, mesenteric lymph nodes, small and large intestine. All mucous membranes and conjunctivae, peritoneum and abdominal fatty tissues were icteric. On external observation. Jaundice, petechial hemorrhages involving mucosal and serosal surfaces of many organs as well as body fat. In the thoracic cavity, the most prominent autopsy findings were obviously extra edematous swelling of all lobes of the lung, hydrothorax and the lung was distended, discolored, solid in texture, and filled with exudate by palpation, The liver was friable, yellowish, and larger than normal, with the gall bladder being markedly distended with dark olive-green or brownish green bile. The abomasum was the most severely affected organ in the alimentary canal, it contains numerous ulcers about 3 mm. in diameter .a few linear ulcers were present on the leaves. There were prominent hemorrhagic ulcers and petechial hemorrhages were seen in the abomasum of the most cases. There were remarkable enlargement of spleen (splenomegaly) were also recorded. The kidneys were congested or dark brown in color and their perirenal fat were yellowish in color. The heart had petechial and hemorrhages on the outer and inner surface of the auricles.
Key word: Cattle, Buffaloes, Theileriosis, polymerase chain reaction, autopsy finding

Toxoplasma gondii is intracellular protozoan parasite that are distributed worldwide and of major economic importance in the livestock industry especially sheep and goats. Sheep and goats are thought to be biological indicators of environmental contamination with T. gondii oocysts. In addition, in developing countries such as Saudi Arabia, where sheep and goat meat is commonly consumed, T. gondii infection in small ruminants may also affect public health risks. So that we estimate the prevalence of T. gondii infections in small ruminants, by using indirect enzyme-linked immunosorbent assay (ELISA) to detect the antibodies to assess the seroprevalence in 130 sheep and 130 goats from Dammam city. A total of 130 sheep were sampled, of which 35 (26.9%) were positive for T. gondii, out of 130 tested goats 31 (23.8%) were positive for T. gondii. Our study also recorded 27 out 52 from the aborted ewes (51.9%) and 22 out 45 from the aborted does (48.8%) were seropositive for anti-T. gondii antibody. Significant differences (p value < 0.0001) were observed among previously aborted females when evaluated as risk factors for T. gondii infection in both ewes and does. In addition, the results revealed that the age in sheep more than 3 years give 31.1% but in goat 23%. Significant differences (p value < 0.0001) were observed among previously ages, sex and the farming system, from the results of this study showed a broad distribution for protozoan parasite (Toxoplasma gondii) in examined sheep and goat flocks. By using ELISA Toxoplasma gondii antibody test kit that provides a rapid, simple, sensitive and specific method for diagnosis of Toxoplasmosis.

Keywords: Toxoplasma gondii; Sheep; Goats; Saudi Arabia; Elisa

Background and Aim: Ginger (Zingiber officinale) has great potential as a growth promoter and immunostimulant in ruminant nutrition. This study assessed the impact of ginger powder supplementation on Ossimi rams’ rumen fermentation, biochemical parameters, and antioxidant levels. Materials and Methods: Fifteen Ossimi rams, aged 10 ± 1.3 months and weighing 30 ± 1.5 kg. Rams were randomly divided into three experimental groups: The control group (G1) received standard feed, while ginger powder (5 g and 7 g/kg body weight [BW] for G2 and G3, respectively) mixed in water was administered to groups G2 and G3 before their standard feed. Results: The control group recorded higher dry matter (DM) intake values (p < 0.05) than the ginger-treated groups. The ginger-treated groups showed superiority (p < 0.05) in weight gain and feed conversion compared to the control group. The digestion coefficients of DM, crude protein, and crude fiber were significantly (p < 0.05) increased by a high dose (7 g/Kg BW) of ginger supplementation, whereas organic matter, ether extract, and nitrogen-free extract digestibility remained unchanged. Compared to the control group, the rams given 5 g of ginger had significantly less (p < 0.05) total protein and globulin in their serum, but the rams given 7 g of ginger had significantly more (p < 0.05) of these proteins. In the ginger groups, these levels were significantly (p < 0.01) lower than those in the control group for serum creatinine, uric acid, urea, total lipids, triglycerides, total cholesterol, glucose, serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and gamma-glutamyl transferase. Rams given ginger had significant growth hormone, insulin-like growth factor-1, total superoxide dismutase, GSH-Px, TAC, immunoglobulin (Ig) A, and IgG enhancement (p < 0.01), and a decrease (p < 0.01) in malondialdehyde concentration compared to the control group. Significant increases in total short-chain volatile fatty acids, acetic, propionic, and isovaleric acids (p < 0.05), and significant decreases in NH3N and protozoa (p < 0.01). Conclusion: Ginger powder (5 g and 7 g) can improve growth, immune responses, antioxidant status, and ruminal parameters in rams. Further study is needed to evaluate the effect of ginger on different types of animals (cow, buffalo, and goat) to develop new feed additives

The present study was conducted to assess the quality of imported frozen liver sold in Assiut markets, Egypt. A total of 100 samples were randomly collected over a 2 months period (January to March, 2020) from poultry slaughter shops, supermarkets and frozen meat markets. The liver samples were evaluated for chemical indicators of spoilage (pH, TVBN "Total Volatile base nitrogen", and TBA "Thiobarbituric acid") and some of the nutritional aspects (percentage of moisture, protein, fat, ash, and carbohydrates, as well as, gross energy (Kcal/100g) and cholesterol content (mg/100g), beside levels of iron (mg/100g). The obtained mean values of pH, TVBN (mg/100 g) and TBA (mg/kg) of the examined samples were 6.38 ± 0.01, 25.76 ± 0.44, and 0.65 ± 0.03, respectively. Of the examined samples, 92 % showed pH value exceeded the permissible limits of Egyptian standards; however, 88 % showed TVBN content within the set limit. The mean values of moisture, protein, fat, ash and carbohydrates (%) were 70.54 ± 0.11, 21.64 ± 0.08, 3.58± 0.12, 1.50 ± 0.018, and 2.74 ± 0.11, respectively. The calculated gross energy mean value was of 133.09 ± 1.06 Kcal/100 g. Additionally, the cholesterol and iron content mean values were 130.85 ± 2.17 mg/100 g and 16.07 ± 0.24 mg/100 g, respectively in the examined imported frozen liver samples. In conclusion, imported frozen liver sold in markets of Assiut city Egypt is of fair quality; it should be consumed sporadically and with care of TBARS, cholesterol and iron potential hazards.
 

Lemongrass (Cymbopogon citratus) essential oil (LEO) and concentrations (1 and 1.5 %) of its nanoemulsion (LGNE) were included in beef burger formula to investigate their effect on shelf life, microbial, chemical indices, and technological properties. The product was frozen at -18±3ºC and examined at intervals (0, 48hrs, 4 weeks, then at a month interval for up to 4 months). GC analysis of LEO revealed that citric acid, verbenol, β-pinene, á-Myrcene and ethyl acetate formulate preponderance of the oil. FTIR, PDI, TEM and cytotoxicity were used for nanoemulsion characterization. The sensory attributes study disclosed that the raw oil drastically impacted the sensory criteria “colour and odour”; though samples with LGNE showed better acceptability. Samples with LEO showed the lowest TBC (P<0.05). Both LEO and LGNE showed great antibacterial effect against TCC. TYMC exhibited a numeral decrease in the count (P>0.05) except for the second month of storage (P<0.05). LGNE showed significant antioxidant efficacy nevertheless LEO samples showed higher TBARs values. TVBN was significant lower in LGNE samples especially in the last 2 months of storage. LGNE controlled the increase in samples pH compared to the control (P<0.05). WHC and cooking yield % showed improvement in the treatment’s samples. As well, diameter loss showed numeral decrease in treatments (P>0.05). In conclusion, LGNE generally improves the sensory and cooking properties of burger, over the LGO or the control samples.