A highly stable ternary complex of iron(III) with 5-(4-nitrophenylazo) salicylic acid (NPAS) and Eosin (Es) in acidic medium at room temperature gave a maximum absorption at 545 nm with a molar absorptivity 2.81×104 L mol−1 cm−1. A spectrophotometric method using these ligands was developed and optimized in terms of pH, stability of the complex, amount of reagent required, sensitivity, linearity, and the effect of various foreign ions was studied. The linear range for iron(III) determination is 0.18–6.0 mg L−1. The method was sensitive, accurate, and all the reagents were stable under the working conditions. Moreover, the method was easy to perform for the determination of iron in pharmaceutical and water samples.

A highly sensitive method is proposed to determine copper(II) ions by forming a stable complex through their
interaction with 4-(2′-benzothiazolylazo)-salicylic acid (BTAS) at room temperature and pH of about 5.0. The
complex gave a maximum absorption at λ = 485 nm with a molar absorptivity coefficient of 2.35⋅104
l/(mol⋅cm). The linear range for the copper determination is 0.63–5.04 mg/l. The method can be applied to
determine copper ions in different biological specimens like some drugs and water samples.

Cisplatin (CP) is considered as a major antineoplastic drug against a broad spectrum of malignancies. CP acts on cancer cells by releasing free radicals which at the same time damage liver and kidney cells. In this study, we aimed to investigate the hepatotoxicity of CP which it may be mediated by oxidative stress and to establish whether some antioxidants, Vit C, DPPD and L-cystiene, may provide protection against CP hepatotoxicity. 40 rats were divided into 5 groups. G1 work as control, G2 injected with CP alone, G3 was injected with CP & Vit C, G4 was injected with CP& DPPD, G5 was injected with CP & L-cystiene. CP-induced oxidative stress was indicated by ↑ LPO and O2- in hepatic tissue and plasma. Also, CP induced decline of SOD, CAT, GST and GGT and a ↓ level of GSH, Vit C and Vit E in hepatic tissue and plasma. Treatment with Vit C, DPPD and L-cysteine in combination with CP restored LPO and O2-, the activities of SOD, GST, CAT and GGT and the content of GSH, Vit C and Vit E to control levels

Cisplatin (CP) is considered as a major antineoplastic drug against a broad spectrum of malignancies. CP acts on cancer cells by releasing free radicals which at the same time damage liver and kidney cells. In this study, we aimed to investigate the hepatotoxicity of CP which it may be mediated by oxidative stress and to establish whether some antioxidants, Vit C, DPPD and L-cystiene, may provide protection against CP hepatotoxicity. 40 rats were divided into 5 groups. G1 work as control, G2 injected with CP alone, G3 was injected with CP & Vit C, G4 was injected with CP& DPPD, G5 was injected with CP & L-cystiene. CP-induced oxidative stress was indicated by ↑ LPO and O2- in hepatic tissue and plasma. Also, CP induced decline of SOD, CAT, GST and GGT and a ↓ level of GSH, Vit C and Vit E in hepatic tissue and plasma. Treatment with Vit C, DPPD and L-cysteine in combination with CP restored LPO and O2-, the activities of SOD, GST, CAT and GGT and the content of GSH, Vit C and Vit E to control levels

Cisplatin (CP) is considered as a major antineoplastic drug against a broad spectrum of malignancies. CP acts on cancer cells by releasing free radicals which at the same time damage liver and kidney cells. In this study, we aimed to investigate the hepatotoxicity of CP which it may be mediated by oxidative stress and to establish whether some antioxidants, Vit C, DPPD and L-cystiene, may provide protection against CP hepatotoxicity. 40 rats were divided into 5 groups. G1 work as control, G2 injected with CP alone, G3 was injected with CP & Vit C, G4 was injected with CP& DPPD, G5 was injected with CP & L-cystiene. CP-induced oxidative stress was indicated by ↑ LPO and O2- in hepatic tissue and plasma. Also, CP induced decline of SOD, CAT, GST and GGT and a ↓ level of GSH, Vit C and Vit E in hepatic tissue and plasma. Treatment with Vit C, DPPD and L-cysteine in combination with CP restored LPO and O2-, the activities of SOD, GST, CAT and GGT and the content of GSH, Vit C and Vit E to control levels