Abstract: Insulin therapy is a cornerstone of treatment in type 1 diabetes among children with the right self-insulin administration. The use of video showing in pediatric training is considered a powerful and attractive technic. This study aimed to evaluate the effectiveness of videoassisted training on insulin self-administration level among adolescents with type 1 diabetes. Quasi experimental research design was adopted. A study was carried out with 40 children from Children University Hospital. A structured interview schedule and observational checklist on selfadministration of insulin were used to collect the data. Children were trained after the pre-test by video demonstration method and post-test was implemented during the first dose of insulin injection after eight days of training. The study found that more than half (67.5%) of the sample was males with mean age of 14±1.70 years. Only 15% of cases were have adequate total knowledge pre-video intervention, compared to about three quarters (72.5%) of them were have adequate total knowledge post-video intervention and about one quarter (22.5%) of cases were have adequate total practice pre-video intervention, and the majority of cases (85.5) were have adequate total practice post-video intervention. Thus, the video-assisted teaching regarding insulin injection regarding self-administration of insulin for diabetic adolescent was effective in enhancing and improving the Knowledge and practice skills among diabetic adolescent.