Communication is the lifeblood of every relationship, while communication climate is the emotional tone of the relationship. Employees can work well within their organizations if there is a relationship and good communication between the organization represented by the management as a supervisor and the employees as subordinates. In order to run organization smoothly and well coordinated, a communication aspect plays role in the organizational life, where every individual in the organization can convey personal interest and it’s solution will be collectively solved. Aim of the Study: This study was conducted with the aim of identifying the communication climate as perceived by nurses and employees, and determining the type of communication climate at Main Assiut University Hospital. This study was carried out by using descriptive study design. The study sample consisted of 266 nurses classified into, 30 bachelor degree nurses, 215 diploma degree nurses and 21 institute nurse, and 115 employees working in different administrative units. Results: Shows that highest mean scores were found among nurses in provisionalism, description, and empathy respectively. While, among employees were found in description, problem-oriented, and empathy respectively with statistically significant differences among the studied subjects as regard to supportive communication climate factors (P0.000). There was a highly statistically significant difference between age of nurses and supportive communication climate scale (P 0.007). Conclusions: In Defensive communication climate: More than one third of nurses were in supportive level (36.8%). While, nearly half of employees were neutral to supportive (47.0%) with a highly statistically significant differences (P 0.000). In Supportive communication climate: Nearly one third of nurses were in supportive to neutral (31.6%). While, nearly half of employees were supportive (46.1%). Recommendations: Based on the forgoing conclusions, the following recommendations are proposed: Encouragement of and planning for participative decision making, teamwork, in-service training program and open communication are recommended to be present in the work units. [Fatma Rushdy Mohamed, Samah Mohamed Abdalla, and Nahed Shawkat Abo-elmaged.