Education is critical to effective, safe life of preschool age and supervisors with health promotion. The purpose of the study is to evaluate the effect of the instructional scheme upon caregiver and/or Supervisors of nursery school knowledge and skills related to health promotion. Fifty caregiver and/or Supervisors in nursery school who receive instruction about health promotion were included in the study. total of 50 caregivers were randomly selected from social region in Assuit .two tools were designed to collect knowledge, and observational check list sheet to measure caregivers practice. The first tool was A structional interview questionnaire to assess the caregiver and/or Supervisors of nursery school knowledge and second tool is observational checklist to performance of health promotion to determine the baseline data and educational needs .. lt was found that there was a great improvement of the studied sample in relation to knowledge and practice related to health promotion .Continuous education and motivation are needed for the maintenance of safe life to the preschool children in nursery school with supervisors about health promotion in the majority of nursery school of Assuit governorate e. The majority of caregiver and/or Supervisors (92%) had poor level of knowledge, Also (86%) had poor level of practice toward health promotion use play, hygiene, rest, exercise feeding. Immunization, prevent of communicable diseases and accident prevention. There are Statistical significant relationship has been found between caregivers knowledge and practice before and after application of training program. Written policy were recommended for each nursery school is suspected. This besides Adequate training program should be designed to increase the caregivers'knowledge