The aim of this study was to assess an educational training program for nurses working in MCH centers in Assiut regarding infection control. The study was conducted in all maternal and child health centers (MCH) in Assiut City. The total number of the study sample in all MCH centers was72 nurses. There are two tools used for data collection: an interview questiOlmaire and an observation checklist. The interview questionnaire was used before and after the program to measure the exact level of knowledge that nurses have about infection such as (definition of sterilization, proper isolation, good infection control, types of sterilization, equipment that may be sterilized in autoclave (6 questions).!t also assesses nurses knowledge about universal precaution related to blood and body fluids (16 questions) and their knowledge about hand washing (3 questions). The second tool (practice) is an observation checklist that was developed for assessing nurses' performance. Data were collected during the period from the beginning of January 2003 to October 2003. Each center takes two months. Results of this study indicated that nearly half (48.6%) of the sample were (48.6%) in the age group 20-30 years ,45.8% were over 30 years. All nurses lacked knowledge related to epidemiology only 40.2% of the nurses under study had sufficient knowledge regarding epidemiology before exposure to the program. The percentage increased to 88.9% after the administration of the program. Also the majority of nurses had adequately prepared on hand washing techniques on the post test with a statistical significant difference of