Severe viral disease was observed on potato crops growing in Assiut governorate, Upper Egypt. Serological and Molecular identification proved the causal pathogen of this disease is Potato virus Y. comparison of nucleotide and amino sequences of coat protein genes of PVY isolate from Assiut (Upper Egypt) and PVY worldwide isolates revealed that PVY-Assiut shared the highest identity with PVY-NTN isolates and this identity was up to 99% at nucleotide level. Phylogenetic analysis confirmed this close relationship among PVY-Assiut and PVY-NTN strain. These data provided a proof that PVY-Assiut is a PVY-NTN strain. PVY-NTN occurrence has been reported before in North of Egypt but this is the first time to be reported in South of Egypt (Upper Egypt). These data proved the wide spread of this virus all over Egypt and indicated to this strain is replacing the old PVY stain especially PVY-O, PVY-N and presents a serious threat to potato production in Egypt. This study proposes that using nucleotide and amino acid sequences of coat protein gene is a potential tool for identification of PVY strains.

Severe viral disease was observed on potato crops growing in Assiut governorate, Upper Egypt. Serological and Molecular identification proved the causal pathogen of this disease is Potato virus Y. comparison of nucleotide and amino sequences of coat protein genes of PVY isolate from Assiut (Upper Egypt) and PVY worldwide isolates revealed that PVY-Assiut shared the highest identity with PVY-NTN isolates and this identity was up to 99% at nucleotide level. Phylogenetic analysis confirmed this close relationship among PVY-Assiut and PVY-NTN strain. These data provided a proof that PVY-Assiut is a PVY-NTN strain. PVY-NTN occurrence has been reported before in North of Egypt but this is the first time to be reported in South of Egypt (Upper Egypt). These data proved the wide spread of this virus all over Egypt and indicated to this strain is replacing the old PVY stain especially PVY-O, PVY-N and presents a serious threat to potato production in Egypt. This study proposes that using nucleotide and amino acid sequences of coat protein gene is a potential tool for identification of PVY strains.

Severe viral-like symptoms were observed on potato plants growing in Assiut governorate (Upper Egypt) during 2010, 2011 and 2012 growing seasons. Typical viral-like particles were observed under Electron microscopy exanimation. These parti- cles were either rod shape about 750 nm in length or bacilliform about 130 nm in length, and resemble Potato virusY (PVY) and Alfalfa mosaic virus (AMV) particles, respectively. Several biological, serological and molecular methods were applied to identify these particles. Serological tests proved that the causal viruses responsible for these symptoms were AMV and PVY. RNA extracted from infected potato as well as healthy potato plants was tested in Reverse Transcription Polymerase Chain Reaction (RT-PCR) using specific primers to amplifycoat protein gene of AMV or PYV. RT-PCR results proved that the symptomatic potato plants are being infected with AMV or PVY, either separately or simultaneously (mixed infection). To the best of our knowledge, this is the first molecular identification of AMV and PVY infecting potato in Assiut governorate, Upper Egypt.

Severe viral-like symptoms were observed on potato plants growing in Assiut governorate (Upper Egypt) during 2010, 2011 and 2012 growing seasons. Typical viral-like particles were observed under Electron microscopy exanimation. These parti- cles were either rod shape about 750 nm in length or bacilliform about 130 nm in length, and resemble Potato virusY (PVY) and Alfalfa mosaic virus (AMV) particles, respectively. Several biological, serological and molecular methods were applied to identify these particles. Serological tests proved that the causal viruses responsible for these symptoms were AMV and PVY. RNA extracted from infected potato as well as healthy potato plants was tested in Reverse Transcription Polymerase Chain Reaction (RT-PCR) using specific primers to amplifycoat protein gene of AMV or PYV. RT-PCR results proved that the symptomatic potato plants are being infected with AMV or PVY, either separately or simultaneously (mixed infection). To the best of our knowledge, this is the first molecular identification of AMV and PVY infecting potato in Assiut governorate, Upper Egypt.

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