Two hundred and forty random samples of milk and some dairy products; Domiati, Kareish cheese and cream were collected from Assiut city, Egypt. The collected samples were examined for the incidence of Acinetobacter species using Leeds Acinetobacter selective medium. The obtained results revealed that Acinetobacter species was isolated from 15% of the raw milk samples, 3.3% of the Domiati cheese samples, 13.3% of the Kareish cheese samples and 13.3% of the cream samples, while couldn’t be detected in the pasteurized milk samples. The isolated species were Acinetobacter baumannii, Acinetobacter haemolyticus, Acinetobacter calcoaceticus and Acinetobacter junii. Acinetobacter baumannii was detected using blaOXA-23-like, blaOXA-51-like, and class 1 integrase genes. The characterization of the Acinetobacter species for the production of lipolytic enzyme was studied. Out of the 27 isolated Acinetobacter species, 23 possessed lipolytic activity. The public health hazard of this microorganism in milk and some dairy products was discussed.

Two hundred and forty random samples of milk and some dairy products; Domiati, Kareish cheese and cream were collected from Assiut city, Egypt. The collected samples were examined for the incidence of Acinetobacter species using Leeds Acinetobacter selective medium. The obtained results revealed that Acinetobacter species was isolated from 15% of the raw milk samples, 3.3% of the Domiati cheese samples, 13.3% of the Kareish cheese samples and 13.3% of the cream samples, while couldn’t be detected in the pasteurized milk samples. The isolated species were Acinetobacter baumannii, Acinetobacter haemolyticus, Acinetobacter calcoaceticus and Acinetobacter junii. Acinetobacter baumannii was detected using blaOXA-23-like, blaOXA-51-like, and class 1 integrase genes. The characterization of the Acinetobacter species for the production of lipolytic enzyme was studied. Out of the 27 isolated Acinetobacter species, 23 possessed lipolytic activity. The public health hazard of this microorganism in milk and some dairy products was discussed.

This study aimed to determine the incidence of E. coli in raw milk and cheese, in addition to isolate and identify Enterotoxigenic E. coli. The existence of heat stable toxin (STh) and heat labile toxin (LT) genes were determined in the isolated strains. This study included 350 samples of raw milk and cheese samples. Different media were used to isolate E. coli and different biochemical tests were used for identification. E. coli was detected in 61.4% of samples. They were tested for the presence of STh and LT genes by PCR. ETEC was detected in 3.7% of E. coli isolates. Only one strain from milk of street samples that found to harbor STh gene. Seven strains were detected in Kareish cheese including two strains harbor LT gene and five strains harbor STh gene.

This study aimed to determine the incidence of E. coli in raw milk and cheese, in addition to isolate and identify Enterotoxigenic E. coli. The existence of heat stable toxin (STh) and heat labile toxin (LT) genes were determined in the isolated strains. This study included 350 samples of raw milk and cheese samples. Different media were used to isolate E. coli and different biochemical tests were used for identification. E. coli was detected in 61.4% of samples. They were tested for the presence of STh and LT genes by PCR. ETEC was detected in 3.7% of E. coli isolates. Only one strain from milk of street samples that found to harbor STh gene. Seven strains were detected in Kareish cheese including two strains harbor LT gene and five strains harbor STh gene.

This study aimed to determine the incidence of E. coli in raw milk and cheese, in addition to isolate and identify Enterotoxigenic E. coli. The existence of heat stable toxin (STh) and heat labile toxin (LT) genes were determined in the isolated strains. This study included 350 samples of raw milk and cheese samples. Different media were used to isolate E. coli and different biochemical tests were used for identification. E. coli was detected in 61.4% of samples. They were tested for the presence of STh and LT genes by PCR. ETEC was detected in 3.7% of E. coli isolates. Only one strain from milk of street samples that found to harbor STh gene. Seven strains were detected in Kareish cheese including two strains harbor LT gene and five strains harbor STh gene.

This study aimed to determine the incidence of E. coli in raw milk and cheese, in addition to isolate and identify Enterotoxigenic E. coli. The existence of heat stable toxin (STh) and heat labile toxin (LT) genes were determined in the isolated strains. This study included 350 samples of raw milk and cheese samples. Different media were used to isolate E. coli and different biochemical tests were used for identification. E. coli was detected in 61.4% of samples. They were tested for the presence of STh and LT genes by PCR. ETEC was detected in 3.7% of E. coli isolates. Only one strain from milk of street samples that found to harbor STh gene. Seven strains were detected in Kareish cheese including two strains harbor LT gene and five strains harbor STh gene.

This study aimed to determine the incidence of E. coli in raw milk and cheese, in addition to isolate and identify Enterotoxigenic E. coli. The existence of heat stable toxin (STh) and heat labile toxin (LT) genes were determined in the isolated strains. This study included 350 samples of raw milk and cheese samples. Different media were used to isolate E. coli and different biochemical tests were used for identification. E. coli was detected in 61.4% of samples. They were tested for the presence of STh and LT genes by PCR. ETEC was detected in 3.7% of E. coli isolates. Only one strain from milk of street samples that found to harbor STh gene. Seven strains were detected in Kareish cheese including two strains harbor LT gene and five strains harbor STh gene.

The study aims to evaluate the potential reproductive toxicity induced by mancozeb fungicide in male rabbits and to examine the ameliorative effect of glutathione (GSH), a non-enzymatic antioxidant, against mancozeb reproductive toxicity. Mancozeb is a member of the dithiocarbamates group currently in use in the management of fungal diseases of plants. To achieve these aims, mature male White New-Zealand rabbits of 4–5 months old were randomly assigned to four groups of 9 animals each: control, mancozeb only, mancozeb and GSH, and GSH only. This study discovered a significant reduction in serum FSH, LH, testosterone and testicular LDH, ACP, and ALP levels in the groups of mancozeb-treated rabbits compared with control. The mancozeb-treated groups also showed significant losses in sperm viability, along with a significant increase in the number of abnormal sperms. Finally, an upregulation in

The study aims to evaluate the potential reproductive toxicity induced by mancozeb fungicide in male rabbits and to examine the ameliorative effect of glutathione (GSH), a non-enzymatic antioxidant, against mancozeb reproductive toxicity. Mancozeb is a member of the dithiocarbamates group currently in use in the management of fungal diseases of plants. To achieve these aims, mature male White New-Zealand rabbits of 4–5 months old were randomly assigned to four groups of 9 animals each: control, mancozeb only, mancozeb and GSH, and GSH only. This study discovered a significant reduction in serum FSH, LH, testosterone and testicular LDH, ACP, and ALP levels in the groups of mancozeb-treated rabbits compared with control. The mancozeb-treated groups also showed significant losses in sperm viability, along with a significant increase in the number of abnormal sperms. Finally, an upregulation in

In the present study, Mycobacterium avium sub sp. paratuberculosis (MAP) infection was detected by serological and molecular methods in herds of dairy cattle. Both milk and blood samples of 88 suspected affected dairy cattle with paratuberculosis were collected from ten dairy farms from different Governorates in Egypt. California Mastitis Test (CMT) was performed on milk samples. Indirect Enzyme Linked Immuno Sorbent Assay (ELISA) was used for detection of MAP antibodies in milk and serum samples. Polymerase Chain Reaction (PCR) was utilized for molecular identification of MAP from milk samples. 7 (7.95%), 33 (37.5%), 26 (29.55%) and 22 (25%) of 88 milk samples were CMT (++), CMT (+), suspicious and negative, respectively. According to ELISA results of milk samples, 24 (27.27%) and 64 (72.73%) were positive and negative, respectively. On the basis of the ELISA results of serum samples, it was found that 26 (29.55%), 1 (1.14%) and 61 (69.32%) were positive, suspicious and negative, respectively. Concerning PCR of milk samples, MAP DNA was detected in 21 (23.86%) samples.