Adults of Rhadinorhynchus niloticus, a member of the Rhadinorhynchidae family, were isolated from the intestines of wild Nile perch (Lates niloticus (Linnaeus, 1758); Perciformes: Latidae) caught from the River Nile and its tributaries in Assiut City, Egypt. The parasite was found freely in the intestinal lumen with a prevalence of 10.71%, and the burden varied from one to five parasites per fish. The mean intensity and abundance were 2.16 ± 0.47 (95% CI: 1.33 to 3.17) and 0.23 ± 0.08 (95% CI: 0.11 to 0.43), respectively. The parasite was described using light and scanning electron microscopy. Molecular species identification as well as phylogenetic relationship analysis of the isolated parasite were achieved by sequencing and comparisons of the mitochondrial cytochrome oxidase C subunit I (COI) and nuclear 18S rRNA genes. The sequences were deposited in GenBank under the accession numbers PP859185 and MZ727194. Furthermore, phylogenetic analysis demonstrated that the parasites emerged from a separate branch belonging to the Rhadinorhynchidae family, which was clearly distinguished from other genospecies.

Amirthalingamia macracantha (Joyeux and Baer, 1935) larvae, a member of the Gryporhynchidae family (Cestoda: Cyclophyllidea), are commonly found in a variety of African fish species, including Nile tilapia (Oreochromis niloticus, Linnaeus, 1758). Nonetheless, little is known regarding their existence in native Egyptian fish species. Two hundred O. niloticus were examined in the current investigation, using standard parasitological techniques to detect the presence of these larvae in different fish organs. According to this study’s findings, A. macracantha larvae were found in 35% of the microscopically examined samples as red nodules, attaching to the serosa of both the anterior and posterior sections of the intestine and stomach, respectively. The mean abundance of the infection was 3.5 ± 0.05 (CI 95% 3.4–3.5), and its mean intensity was 10 ± 1.7 (CI 95% 8.2–11.7). The existence of two rows of twenty rostellar hooks of varying lengths is the main characteristic of the parasite. The upper row consisted of four large hooks and six smaller ones, while the lower row contained smaller hooks of consistent length. According to histopathological analysis, the larvae with a hook-and-spiny rostellum were affixed to the stomach serosa of O. niloticus. Additionally, there was a notable presence of degranulated mast cells and lymphocytes of varying sizes within the stomach’s lamina propria, submucosa, and blood vessels, as well as a loss of gastric epithelium, fibrosis, and enlargement of submucosal lymph vessels. This study offers a succinct description of this larval stage, representing the first reported occurrence of its presence in Egyptian O. niloticus.

Bovine papular stomatitis (BPS) is a widespread epitheliotropic viral disease affects ruminants of all ages and considered a serious risk to public health. In Assiut governorate, there is little information on BPS despite their financial losses. Thus, the goal of this study was to confirm diagnosis on a molecular base, describe the clinical findings of the disease, and study the relationship between some factors that may play a role in the spread of the disease. The present investigation was conducted on 39 cattle and 11 buffaloes that belonged to different villages in Assiut governorate, Egypt. Whole blood and oral lesions swabs samples were taken for laboratory testing. Semi-nested polymerase chain reaction (semi-nested PCR) had been used for detection of BPS virus (BPSV). The viral DNA was detected in 32 cattle and 8 buffaloes. The result of clinical examination indicated that the clinical signs of BPS were oral (ulcers in gum, palate, papillae & tongue), commissure, muzzle, and nostrils lesions with one or more of other clinical signs such as fever, diarrhea, enlarged superficial lymph nodes, corneal opacity, respiratory distress and skin lesions in studied animals. Studying the effect of some factors on the spread of the disease revealed that the prevalence was significantly higher in middle areas of the governorate than in other areas. To lower the prevalence of BPSV, it is recommended to avoid eating hard hay and reeds that cause small abrasion in oral cavity and aid increasing frequency of BPSV infection.

Bovine ephemeral fever (BEF) is a dangerous febrile viral disease that affects cattle and causes large financial losses. The aims of this study were to study clinical findings of BEF virus (BEFV) infection, identify certain risk factors and evaluate infected cattle’s mineral changes. One hundred and seven cattle were used in this investigation. Whole blood and serum samples were collected for laboratory analysis. Reverse transcriptase polymerase chain reaction (RT-PCR) had been employed for BEFV diagnosis. The observed clinical findings of BEFV infection in cattle were fever, respiratory manifestation (serous nasal discharge & cough), lacrimation, lameness, enlarged of superficial lymph nodes, drop of milk production, ruminal stasis, and recumbency. Of the studied diseased cattle, 43 (43%) of 100 whole blood samples showed molecularly positive results for G1 gene. The infection rate of BEFV had no significant variation by locality, age, sex, breed and season in the studied diseased cattle. Serum calcium level of confirmed BEFV positive samples was significantly lower (p<0.001) than serum calcium level in clinically healthy ones. Serum phosphorus and magnesium levels in BEFV infected cattle did not differ significantly (P<0.05) from that of clinically healthy ones. The importance of establishing efficient prevention and control strategies in Egypt must be emphasized in order to reduce the prevalence of BEFV infection in cattle.

Cisplatin is among the most frequently utilized drugs for addressing malignant tumors, yet it can lead to organ harm, especially hepatotoxicity and nephrotoxicity. Furthermore, the anticoagulant rivaroxaban could poten­ tially cause injury to the liver and kidneys. This research aimed to examine the protective benefits of nebivolol, known for its pleiotropic and tissue-protective characteristics, against the harmful effects of rivaroxaban and cisplatin on the liver and kidneys. Male rats received cisplatin and/or rivaroxaban, and we evaluated hepato­ toxicity and nephrotoxicity by measuring serum concentrations of AST, ALT, LDH, albumin, bilirubin, creatinine, and blood urea. We also measured MDA, GSH, GPx, NO, TNF-α, and IL-6 in kidney and liver homogenates. Histopathological analysis was performed on liver and kidney tissue sections, and immunohistochemical detection of caspase 3 in liver tissue and NF-κB in kidney tissue was conducted. Our findings demonstrated that nebivolol supported the preservation of the liver and kidney structure and function by reducing the biochemical and pathological alterations caused by cisplatin and rivaroxaban. Nebivolol decreased the elevations in MDA, TNF-α, and IL-6 levels while maintaining GSH, GPx, and NO levels in liver and kidney tissues. Moreover, nebivolol lowered the levels of caspase-3 in the liver and NF-κB in the kidneys. In conclusion, our study indicates that nebivolol protects the liver and kidneys from the detrimental effects of cisplatin and rivaroxaban. 
 

Fibromyalgia syndrome (FMS) is a chronic, multidimensional musculoskeletal condition distinguished by severe nociceptive dysfunction, persistent fatigue, sleep disruptions, cognitive deficits, and emotional instability. Although valproic acid (VPA) has been used to treat epilepsy and bipolar disorder, its efficacy in altering neuropathic pain pathways remains unclear. In this investigation, we assessed the neuromodulatory characteristics of VPA (300 mg/kg, intraperitoneally) in an established FMS rat model, with a focus on neuroinflammation, oxidative stress, and glial activation. Behavioral evaluations for thermal hyperalgesia (paw withdrawal latency, PWL) and mechanical allodynia (paw withdrawal threshold, PWT) were performed at baseline (day 0), after induction (day 5), and at various intervals following VPA administration. Neurochemical evaluations demonstrated that VPA markedly diminished FMS-induced elevations in malondialdehyde (MDA), nitric oxide (NO), and pro-inflammatory cytokines such as tumour necrosis factor-α (TNF-α), interleukin (IL)-1β, and interleukin (IL)-6, while restoring antioxidant defences, such as glutathione (GSH) and superoxide dismutase (SOD). Histopathological examination demonstrated reduced neuronal degeneration and decreased immunoreactivity of glial fibrillary acidic protein (GFAP). These findings indicate that VPA reduces FMS-related pain and neuroinflammatory characteristics through antioxidative and glial-modulating mechanisms, indicating its potential for therapeutic repurposing in neuropathic pain syndromes 
 

Stainless steel tubes and pipes are vital in dairy processing but require frequent cleaning, leading to notable energy consumption and environmental impact. This study delves into the cleanability of wet milk deposits at temperatures of 40°C and 60°C on an exceptionally smooth internal surface achieved through magnetic abrasive finishing (MAF) with a surface roughness of 0.01 μm R_a. We compare this surface with non-MAF tubes having surface roughness values of 0.37 and 3.7 μm R_a. To assess cleaning effectiveness, the study measured milk and protein residue removal after deposition and cleaning processes, employing a cleaning solution flow pattern at Reynolds numbers (Re) of 659–1318. Results indicate that smoother surfaces, particularly those with roughness values of 0.01 and 0.37 μm R_a, significantly enhance cleanability at 40°C. This leads to reductions (p < 0.05) of 34.5% and 22.6% for milk deposits and 27.9% and 22.7% for protein deposits, respectively, compared to tube surfaces with a roughness level of 3.7 μm R_a. These findings underscore the potential of highly smooth surfaces to improve cleanability below protein denaturation temperatures. Furthermore, the MAF tube with a roughness of 0.01 μm R_a exhibited nonsignificant reductions of 15.4% and 6.7% compared to the 0.37 μm R_a surface. The smoothing effect on the cleanability of milk and protein deposits was enhanced compared with the higher temperature condition. By addressing the challenges of routine cleaning, the study highlights MAF as a technology that optimizes surface quality in dairy processing equipment, addressing environmental and energy-related concerns.

Anaerobic digestion (AD) is a promising technology for converting organic waste into renewable energy, but its industrial implementation is often constrained by ammonia inhibition in nitrogen-rich feedstocks, which undermines both process stability and economic viability. Addressing this challenge is crucial for ensuring sustainable, financially resilient waste-to-energy systems. We hypothesized that the strategic addition of bamboo biochar (BBC) could mitigate ammonia stress while promoting a more robust microbial community, thereby enhancing both environmental and economic performance. To test this, batch experiments were conducted to determine optimum BBC dosages, followed by semi-continuous trials using 6.25 g/L BBC over four operational phases (Runs1–4), during which NH₄⁺-N was gradually increased from 2000 to 5000 mg/L. The biochar-amended system maintained stable performance under conditions that caused control reactors to fail, with a maximum 1447 % increase in methane production observed during the 4000 mg/L NH₄⁺-N phase. Mechanistic analysis revealed that BBC acted primarily by enriching syntrophic bacteria and hydrogenotrophic methanogens, enabling a stable syntrophic acetate oxidation pathway. Enhancing microbial resilience through biochar addition directly improves financial stability, a critical factor for industrial adoption. The biochar-added system achieved consistent profits of USD 8.08–16.27/m³ reactor/month, underscoring strong business potential in scalable waste-to-energy systems. Optimizing biochar dosing and evaluating full-scale implementation could further advance globally relevant, economically viable circular bioeconomy solutions.

This study explores the interactions between microbial communities, antibiotic resistance, and biogas production in anaerobic digestion systems, focusing on the acidogenic (AP) and methanogenic (MP) phases under varying organic loads, cefazolin (CEZ) exposure, and biochar supplementation. High organic loading (10 g/L glucose) significantly suppressed CEZ-resistant bacteria (CEZ-r) during the AP phase. However, their abundance markedly rebounded in MP, rising from 0.30 % to 36.28 % in control, indicating phase-specific dynamics. CEZ residues increased CEZ-r by 2.49 % and 9.30 % at 0 and 5 g/L glucose during AP. Although AP suppressed CEZ-r to 0.23 % in the CEZ-added reactor at 10 g/L glucose, MP rebounded CEZ-r to 8.30 %. In addition, CEZ exposure reduced methane yields by up to 28.14 %, likely due to the suppression of Methanosaetaceae and impaired acetic acid conversion. In contrast, biochar addition effectively reduced CEZ-r abundance to below 1.00 % at moderate to high organic loads and alleviated CEZ-induced inhibition on methane production. Biochar also enhanced Methanosaetaceae abundance (up to +6.55 %) compared to the control and promoted more efficient substrate utilization, possibly by facilitating direct interspecies electron transfer. These findings emphasize the role of organic load and digestion phase in shaping antibiotic resistance and system performance. Furthermore, biochar addition effectively mitigates the negative impacts of antibiotic residues, stabilizes microbial communities, and enhances biogas production.