The present study aimed to investigate the effect of two levels of carob powder (10% and 20%) on serum glucose and lipid profile. Forty male Sprague-Dawley rats were divided into two groups. The First group control negative group consisted of (10) rats fed on basal diet. The second main group consisted of (30) rats were injected intraperitoneal with alloxan monohydrate in single dose (170 mg/Kg) body weight. The second main group was divided into three subgroups (10 rats each). Subgroup (1); diabetic group was fed on basal diet only control positive group, Subgroup (2); diabetic group was fed on basal diet plus 10% carob powder and subgroup (3); diabetic group was fed on basal diet plus 20% carob powder for six weeks. At the end of the experimental period, serum samples was collected to measure serum glucose (SG), total cholesterol (TC), triglyceride (TG), high-density lipoproteins (HDL), lowdensity lipoproteins (LDL) and very-low-density lipoproteins (VLDL). The obtained results revealed that feeding rat's 10% and 20% carob powder improved serum glucose and lipid profile parameters in the experimental rats.