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The last decades have been witness of an ever-growing interests for the synthesis and application of metal–organic frameworks (MOFs). However, most of the current synthetic procedures produce MOFs in powder state. In this work, hybrid foams were fabricated via in situ synthesis of leaf-like zeolitic imidazolate frameworks (ZIF-L) into nanocellulose at room temperature using water as solvent, followed by a gelatin matrix incorporation and freeze-drying. The foams are ultralight weight and are highly porous with densities ranging from 19.18 to 37.4 kg·m−3. The shapeability, hierarchical porosity, and low density of the formed foams offer promising potentials for applications such as CO2 sorption. The dispersion of ZIF-L into the cellulose network increases the material accessibility and may open new venues for further MOFs processing.

This work presents a second-to-none method for Taxol isolation from the Endophytic fungus Cladosporium
sphaerospermum (AUMC 6896) and the Entomopathogenic fungus Metarizium anisopliae (AUMC 5130). The
extracts were analyzed by high performance liquid chromatography (HPLC) and Liquid chromatography coupled
to tandem mass spectrometry (LC-MS/MS) using positive electrospray ionization (ESI) in the multiple reaction
monitoring (MRM) mode. This is rapid, consistent, reproducible, accurate, and sensitive for quantifying Taxol
across multiple samples. The yield of crude Taxol product obtained from Potato Dextrose broth (PDB) medium
inoculated with Cladosporium sphaerospermu and Metarizium anisopliae was found to be 3.732, and
0.0023 μg L−1 respectively. The yield can be improved by adding ammonium acetate or salicylic acid to the
culture broth. Addition of ammonium acetate (AA) (20 mg L−1
) to culture media resulted in an increase of Taxol
yield to 30.365 and 27.289 μg L−1 respectively. Production of Taxol was 29.844 and 67.254 μg L−1 for the two
fungus species when ammonium acetate was substituted by 90 mg L−1 salicylic acid (SA). Adding both AA
(20 mg L−1
) and SA (90 mg L−1
) to the culture media resulted in an increase of the Taxol yield to 4.054 and
116.373 μg L−1 respectively.
Our proposed analytical method offers very fast (3 min) quantitation of Taxol in comparison with other
published methods. These findings represent a new bioprospecting of the endophytic fungi that may serve as a
potential material for the production of Taxol for anticancer treatment.

This work presents a second-to-none method for Taxol isolation from the Endophytic fungus Cladosporium
sphaerospermum (AUMC 6896) and the Entomopathogenic fungus Metarizium anisopliae (AUMC 5130). The
extracts were analyzed by high performance liquid chromatography (HPLC) and Liquid chromatography coupled
to tandem mass spectrometry (LC-MS/MS) using positive electrospray ionization (ESI) in the multiple reaction
monitoring (MRM) mode. This is rapid, consistent, reproducible, accurate, and sensitive for quantifying Taxol
across multiple samples. The yield of crude Taxol product obtained from Potato Dextrose broth (PDB) medium
inoculated with Cladosporium sphaerospermu and Metarizium anisopliae was found to be 3.732, and
0.0023 μg L−1 respectively. The yield can be improved by adding ammonium acetate or salicylic acid to the
culture broth. Addition of ammonium acetate (AA) (20 mg L−1
) to culture media resulted in an increase of Taxol
yield to 30.365 and 27.289 μg L−1 respectively. Production of Taxol was 29.844 and 67.254 μg L−1 for the two
fungus species when ammonium acetate was substituted by 90 mg L−1 salicylic acid (SA). Adding both AA
(20 mg L−1
) and SA (90 mg L−1
) to the culture media resulted in an increase of the Taxol yield to 4.054 and
116.373 μg L−1 respectively.
Our proposed analytical method offers very fast (3 min) quantitation of Taxol in comparison with other
published methods. These findings represent a new bioprospecting of the endophytic fungi that may serve as a
potential material for the production of Taxol for anticancer treatment.

This work presents a second-to-none method for Taxol isolation from the Endophytic fungus Cladosporium
sphaerospermum (AUMC 6896) and the Entomopathogenic fungus Metarizium anisopliae (AUMC 5130). The
extracts were analyzed by high performance liquid chromatography (HPLC) and Liquid chromatography coupled
to tandem mass spectrometry (LC-MS/MS) using positive electrospray ionization (ESI) in the multiple reaction
monitoring (MRM) mode. This is rapid, consistent, reproducible, accurate, and sensitive for quantifying Taxol
across multiple samples. The yield of crude Taxol product obtained from Potato Dextrose broth (PDB) medium
inoculated with Cladosporium sphaerospermu and Metarizium anisopliae was found to be 3.732, and
0.0023 μg L−1 respectively. The yield can be improved by adding ammonium acetate or salicylic acid to the
culture broth. Addition of ammonium acetate (AA) (20 mg L−1
) to culture media resulted in an increase of Taxol
yield to 30.365 and 27.289 μg L−1 respectively. Production of Taxol was 29.844 and 67.254 μg L−1 for the two
fungus species when ammonium acetate was substituted by 90 mg L−1 salicylic acid (SA). Adding both AA
(20 mg L−1
) and SA (90 mg L−1
) to the culture media resulted in an increase of the Taxol yield to 4.054 and
116.373 μg L−1 respectively.
Our proposed analytical method offers very fast (3 min) quantitation of Taxol in comparison with other
published methods. These findings represent a new bioprospecting of the endophytic fungi that may serve as a
potential material for the production of Taxol for anticancer treatment.