The present study examines the possibility of increasing the levels of some bioactive compounds
(a-carotene, phycopiliprotein and total lipids) in Spirulina sp. isolated from Wadi El Natron lake (Egypt)
and cultivated in modified Zarrouk's medium under standard growth condition and abiotic stress
including change in culturing condition as (pH, temperature, light intensity), nutrient limitation (-N, -P, -S)
and salinity. The yield production of a-carotene was enhanced with 0.9M NaCl, phosphorus deficiency
and at pH 6. Total lipids were also enhanced in all conditions under tested especially with 0.9M NaCl,
light intensity 1 4.52 Xmol photon.m-2.s-1 . Phycobiliproteins fractions (phycocyanin, allophycocyanine and
phycoerethrine) were elevated with 0.3, 0.6M NaCl as well as phosphorus, nitrogen and sulphur
deficiency. Antioxidant enzymatic activities of Spirulina sp. were elevated with all studied abiotic stress
factor.

The present study examines the possibility of increasing the levels of some bioactive compounds
(a-carotene, phycopiliprotein and total lipids) in Spirulina sp. isolated from Wadi El Natron lake (Egypt)
and cultivated in modified Zarrouk's medium under standard growth condition and abiotic stress
including change in culturing condition as (pH, temperature, light intensity), nutrient limitation (-N, -P, -S)
and salinity. The yield production of a-carotene was enhanced with 0.9M NaCl, phosphorus deficiency
and at pH 6. Total lipids were also enhanced in all conditions under tested especially with 0.9M NaCl,
light intensity 1 4.52 Xmol photon.m-2.s-1 . Phycobiliproteins fractions (phycocyanin, allophycocyanine and
phycoerethrine) were elevated with 0.3, 0.6M NaCl as well as phosphorus, nitrogen and sulphur
deficiency. Antioxidant enzymatic activities of Spirulina sp. were elevated with all studied abiotic stress
factor.

The present study examines the possibility of increasing the levels of some bioactive compounds
(a-carotene, phycopiliprotein and total lipids) in Spirulina sp. isolated from Wadi El Natron lake (Egypt)
and cultivated in modified Zarrouk's medium under standard growth condition and abiotic stress
including change in culturing condition as (pH, temperature, light intensity), nutrient limitation (-N, -P, -S)
and salinity. The yield production of a-carotene was enhanced with 0.9M NaCl, phosphorus deficiency
and at pH 6. Total lipids were also enhanced in all conditions under tested especially with 0.9M NaCl,
light intensity 1 4.52 Xmol photon.m-2.s-1 . Phycobiliproteins fractions (phycocyanin, allophycocyanine and
phycoerethrine) were elevated with 0.3, 0.6M NaCl as well as phosphorus, nitrogen and sulphur
deficiency. Antioxidant enzymatic activities of Spirulina sp. were elevated with all studied abiotic stress
factor.

Reaction of 4chlorocoumarin3carbonitrile with ethyl thioglycolate and ethyl glycinate hydrochloride
leads to a series of title products. Hydrazinolysis of amino thienocoumarin carboxylate afforded the hydrazi
no derivative which underwent various reactions to build new heterocyclic rings containing thienocoumarin
moiety. Chloro acetylation of aminoester compound afforded the chloro acetyl amino which underwent nu
cleophilic substitution reactions various amines. The following treatment with formaldehyde under Mannich
conditions afforded the corresponding imidazo derivatives. Reaction of chloroacetylamino with potassium
thiocyanate yielded ethylpyrimidothieno coumarin sulfanylacetate which was used as a versatile precursor for
synthesis of other heterocycles. On the other hand, reaction of chloro coumarin carbonitrile with hydrazine
gave the aminopyrazolocoumaine which reacted with bifunctionally compounds to give the substituted py
rimido derivatives. Diazotization and coupling of aminopyrazole with ethylcyanoacetate yielded ethylami
notriazinopyrazolocoumarine carboxylate. Several of the compounds obtained demonstrated considerable
antifungal and antibacterial activity in the in vitro test systems.

Reaction of 4chlorocoumarin3carbonitrile with ethyl thioglycolate and ethyl glycinate hydrochloride
leads to a series of title products. Hydrazinolysis of amino thienocoumarin carboxylate afforded the hydrazi
no derivative which underwent various reactions to build new heterocyclic rings containing thienocoumarin
moiety. Chloro acetylation of aminoester compound afforded the chloro acetyl amino which underwent nu
cleophilic substitution reactions various amines. The following treatment with formaldehyde under Mannich
conditions afforded the corresponding imidazo derivatives. Reaction of chloroacetylamino with potassium
thiocyanate yielded ethylpyrimidothieno coumarin sulfanylacetate which was used as a versatile precursor for
synthesis of other heterocycles. On the other hand, reaction of chloro coumarin carbonitrile with hydrazine
gave the aminopyrazolocoumaine which reacted with bifunctionally compounds to give the substituted py
rimido derivatives. Diazotization and coupling of aminopyrazole with ethylcyanoacetate yielded ethylami
notriazinopyrazolocoumarine carboxylate. Several of the compounds obtained demonstrated considerable
antifungal and antibacterial activity in the in vitro test systems.

Reaction of 4chlorocoumarin3carbonitrile with ethyl thioglycolate and ethyl glycinate hydrochloride
leads to a series of title products. Hydrazinolysis of amino thienocoumarin carboxylate afforded the hydrazi
no derivative which underwent various reactions to build new heterocyclic rings containing thienocoumarin
moiety. Chloro acetylation of aminoester compound afforded the chloro acetyl amino which underwent nu
cleophilic substitution reactions various amines. The following treatment with formaldehyde under Mannich
conditions afforded the corresponding imidazo derivatives. Reaction of chloroacetylamino with potassium
thiocyanate yielded ethylpyrimidothieno coumarin sulfanylacetate which was used as a versatile precursor for
synthesis of other heterocycles. On the other hand, reaction of chloro coumarin carbonitrile with hydrazine
gave the aminopyrazolocoumaine which reacted with bifunctionally compounds to give the substituted py
rimido derivatives. Diazotization and coupling of aminopyrazole with ethylcyanoacetate yielded ethylami
notriazinopyrazolocoumarine carboxylate. Several of the compounds obtained demonstrated considerable
antifungal and antibacterial activity in the in vitro test systems.

Reaction of 4chlorocoumarin3carbonitrile with ethyl thioglycolate and ethyl glycinate hydrochloride
leads to a series of title products. Hydrazinolysis of amino thienocoumarin carboxylate afforded the hydrazi
no derivative which underwent various reactions to build new heterocyclic rings containing thienocoumarin
moiety. Chloro acetylation of aminoester compound afforded the chloro acetyl amino which underwent nu
cleophilic substitution reactions various amines. The following treatment with formaldehyde under Mannich
conditions afforded the corresponding imidazo derivatives. Reaction of chloroacetylamino with potassium
thiocyanate yielded ethylpyrimidothieno coumarin sulfanylacetate which was used as a versatile precursor for
synthesis of other heterocycles. On the other hand, reaction of chloro coumarin carbonitrile with hydrazine
gave the aminopyrazolocoumaine which reacted with bifunctionally compounds to give the substituted py
rimido derivatives. Diazotization and coupling of aminopyrazole with ethylcyanoacetate yielded ethylami
notriazinopyrazolocoumarine carboxylate. Several of the compounds obtained demonstrated considerable
antifungal and antibacterial activity in the in vitro test systems.