Setaria equina crude antigen was used for the first time in diagnosis of human bancroftian filariasis by using ELISA. The adult worms of Setaria equina were collected washed grounded in cold PBS, using homogenizer, and centrifuged. The supernatant was used as the adult antigen. Fractionation of the whole S.equina crude antigen was done by polyacrylamide gel electrophoresis. Three bands were obtained at 25.4 KD, 27.5 KD and 29.9 KD. The prepared whole S.equina crude antigen was used in ELLSA test for diagnosis of humans bancroftian filariasis and S. equina infection in donkey as well. The sensitivity and specificity of the test in equines were 100% and 80% respectively, while in human bancroftian filariasis the sensitivity and specificity, was 71% and 71.43% respectively. ELISA proved to be reliable and sensitive test and could be used in sero diagnosis of W. bancrofti and S.equina infection in both humans and equines as well. This test proved to overcome the problem arising from sampling the patients at the midnight, furthermore antigen prepared from S.equina adult worm was more economic than the imported other antigens prepared from D.immitis and others.

Setaria equina crude antigen was used for the first time in diagnosis of human bancroftian filariasis by using ELISA. The adult worms of Setaria equina were collected washed grounded in cold PBS, using homogenizer, and centrifuged. The supernatant was used as the adult antigen. Fractionation of the whole S.equina crude antigen was done by polyacrylamide gel electrophoresis. Three bands were obtained at 25.4 KD, 27.5 KD and 29.9 KD. The prepared whole S.equina crude antigen was used in ELLSA test for diagnosis of humans bancroftian filariasis and S. equina infection in donkey as well. The sensitivity and specificity of the test in equines were 100% and 80% respectively, while in human bancroftian filariasis the sensitivity and specificity, was 71% and 71.43% respectively. ELISA proved to be reliable and sensitive test and could be used in sero diagnosis of W. bancrofti and S.equina infection in both humans and equines as well. This test proved to overcome the problem arising from sampling the patients at the midnight, furthermore antigen prepared from S.equina adult worm was more economic than the imported other antigens prepared from D.immitis and others.

Setaria equina crude antigen was used for the first time in diagnosis of human bancroftian filariasis by using ELISA. The adult worms of Setaria equina were collected washed grounded in cold PBS, using homogenizer, and centrifuged. The supernatant was used as the adult antigen. Fractionation of the whole S.equina crude antigen was done by polyacrylamide gel electrophoresis. Three bands were obtained at 25.4 KD, 27.5 KD and 29.9 KD. The prepared whole S.equina crude antigen was used in ELLSA test for diagnosis of humans bancroftian filariasis and S. equina infection in donkey as well. The sensitivity and specificity of the test in equines were 100% and 80% respectively, while in human bancroftian filariasis the sensitivity and specificity, was 71% and 71.43% respectively. ELISA proved to be reliable and sensitive test and could be used in sero diagnosis of W. bancrofti and S.equina infection in both humans and equines as well. This test proved to overcome the problem arising from sampling the patients at the midnight, furthermore antigen prepared from S.equina adult worm was more economic than the imported other antigens prepared from D.immitis and others.

ABSTRACT Crude antigen prepared from adult Gigantobilharzia worms was used - for the first time - to immunize Balb/c mice against challenge infection with Schistosoma mansoni. Results of immunization were estimated by appearance of eggs in stool, antibodies in serum by Indirect Haemagglutination Test, counting the number of eggs and adults in liver and intestinal veins and tissues, estimation of the number and size of granulomas in the liver as well as description of adults deformities by ordinary and scanning electron microscopes. Obvious reduction in all these parameters was recorded in immunized mice. Further studies were recommended through the use of purified bird schistosomes antigens.

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