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n order to elucidate the present status of filariasis in Assiut Governorate, humans dogs, equines, camels and cattle were surveyed. No Wuchereria bancrofti microfilariae could be detected in 400 human blood samples collected from the endemic areas between 10 p.m. and 2 a.m. On the other hand, five clinical cases of elephantiasis were recorded, which were amicrofilaraemic. Dirofilaria repens non sheathed microfilaria was recorded for the first time in Egypt from the peripheral blood of four out 30 (13.3%) dogs. Onchocerca cervicalis microfilaria was detected in the umblical skin in one out of 30 (3.3%) donkeys. The non sheathed Onchocerca reticulata and the sheathed Setaria equina microfilariae were found in the peripheral blood of three (6%) and eight (16%) out of 50 donkeys respectively. Dipetalonema evansi microfilariae was recovered in peripheral blood of one out of 15 (6.6%) camels. No microfilariae could be detected in the umbilical skins of 130 cows and buffaloes. The role of the above mentioned microfilariae in producing human morbidity is discussed

n order to elucidate the present status of filariasis in Assiut Governorate, humans dogs, equines, camels and cattle were surveyed. No Wuchereria bancrofti microfilariae could be detected in 400 human blood samples collected from the endemic areas between 10 p.m. and 2 a.m. On the other hand, five clinical cases of elephantiasis were recorded, which were amicrofilaraemic. Dirofilaria repens non sheathed microfilaria was recorded for the first time in Egypt from the peripheral blood of four out 30 (13.3%) dogs. Onchocerca cervicalis microfilaria was detected in the umblical skin in one out of 30 (3.3%) donkeys. The non sheathed Onchocerca reticulata and the sheathed Setaria equina microfilariae were found in the peripheral blood of three (6%) and eight (16%) out of 50 donkeys respectively. Dipetalonema evansi microfilariae was recovered in peripheral blood of one out of 15 (6.6%) camels. No microfilariae could be detected in the umbilical skins of 130 cows and buffaloes. The role of the above mentioned microfilariae in producing human morbidity is discussed

n order to elucidate the present status of filariasis in Assiut Governorate, humans dogs, equines, camels and cattle were surveyed. No Wuchereria bancrofti microfilariae could be detected in 400 human blood samples collected from the endemic areas between 10 p.m. and 2 a.m. On the other hand, five clinical cases of elephantiasis were recorded, which were amicrofilaraemic. Dirofilaria repens non sheathed microfilaria was recorded for the first time in Egypt from the peripheral blood of four out 30 (13.3%) dogs. Onchocerca cervicalis microfilaria was detected in the umblical skin in one out of 30 (3.3%) donkeys. The non sheathed Onchocerca reticulata and the sheathed Setaria equina microfilariae were found in the peripheral blood of three (6%) and eight (16%) out of 50 donkeys respectively. Dipetalonema evansi microfilariae was recovered in peripheral blood of one out of 15 (6.6%) camels. No microfilariae could be detected in the umbilical skins of 130 cows and buffaloes. The role of the above mentioned microfilariae in producing human morbidity is discussed

n order to elucidate the present status of filariasis in Assiut Governorate, humans dogs, equines, camels and cattle were surveyed. No Wuchereria bancrofti microfilariae could be detected in 400 human blood samples collected from the endemic areas between 10 p.m. and 2 a.m. On the other hand, five clinical cases of elephantiasis were recorded, which were amicrofilaraemic. Dirofilaria repens non sheathed microfilaria was recorded for the first time in Egypt from the peripheral blood of four out 30 (13.3%) dogs. Onchocerca cervicalis microfilaria was detected in the umblical skin in one out of 30 (3.3%) donkeys. The non sheathed Onchocerca reticulata and the sheathed Setaria equina microfilariae were found in the peripheral blood of three (6%) and eight (16%) out of 50 donkeys respectively. Dipetalonema evansi microfilariae was recovered in peripheral blood of one out of 15 (6.6%) camels. No microfilariae could be detected in the umbilical skins of 130 cows and buffaloes. The role of the above mentioned microfilariae in producing human morbidity is discussed

Setaria equina crude antigen was used for the first time in diagnosis of human bancroftian filariasis by using ELISA. The adult worms of Setaria equina were collected washed grounded in cold PBS, using homogenizer, and centrifuged. The supernatant was used as the adult antigen. Fractionation of the whole S.equina crude antigen was done by polyacrylamide gel electrophoresis. Three bands were obtained at 25.4 KD, 27.5 KD and 29.9 KD. The prepared whole S.equina crude antigen was used in ELLSA test for diagnosis of humans bancroftian filariasis and S. equina infection in donkey as well. The sensitivity and specificity of the test in equines were 100% and 80% respectively, while in human bancroftian filariasis the sensitivity and specificity, was 71% and 71.43% respectively. ELISA proved to be reliable and sensitive test and could be used in sero diagnosis of W. bancrofti and S.equina infection in both humans and equines as well. This test proved to overcome the problem arising from sampling the patients at the midnight, furthermore antigen prepared from S.equina adult worm was more economic than the imported other antigens prepared from D.immitis and others.

Setaria equina crude antigen was used for the first time in diagnosis of human bancroftian filariasis by using ELISA. The adult worms of Setaria equina were collected washed grounded in cold PBS, using homogenizer, and centrifuged. The supernatant was used as the adult antigen. Fractionation of the whole S.equina crude antigen was done by polyacrylamide gel electrophoresis. Three bands were obtained at 25.4 KD, 27.5 KD and 29.9 KD. The prepared whole S.equina crude antigen was used in ELLSA test for diagnosis of humans bancroftian filariasis and S. equina infection in donkey as well. The sensitivity and specificity of the test in equines were 100% and 80% respectively, while in human bancroftian filariasis the sensitivity and specificity, was 71% and 71.43% respectively. ELISA proved to be reliable and sensitive test and could be used in sero diagnosis of W. bancrofti and S.equina infection in both humans and equines as well. This test proved to overcome the problem arising from sampling the patients at the midnight, furthermore antigen prepared from S.equina adult worm was more economic than the imported other antigens prepared from D.immitis and others.

Setaria equina crude antigen was used for the first time in diagnosis of human bancroftian filariasis by using ELISA. The adult worms of Setaria equina were collected washed grounded in cold PBS, using homogenizer, and centrifuged. The supernatant was used as the adult antigen. Fractionation of the whole S.equina crude antigen was done by polyacrylamide gel electrophoresis. Three bands were obtained at 25.4 KD, 27.5 KD and 29.9 KD. The prepared whole S.equina crude antigen was used in ELLSA test for diagnosis of humans bancroftian filariasis and S. equina infection in donkey as well. The sensitivity and specificity of the test in equines were 100% and 80% respectively, while in human bancroftian filariasis the sensitivity and specificity, was 71% and 71.43% respectively. ELISA proved to be reliable and sensitive test and could be used in sero diagnosis of W. bancrofti and S.equina infection in both humans and equines as well. This test proved to overcome the problem arising from sampling the patients at the midnight, furthermore antigen prepared from S.equina adult worm was more economic than the imported other antigens prepared from D.immitis and others.

Setaria equina crude antigen was used for the first time in diagnosis of human bancroftian filariasis by using ELISA. The adult worms of Setaria equina were collected washed grounded in cold PBS, using homogenizer, and centrifuged. The supernatant was used as the adult antigen. Fractionation of the whole S.equina crude antigen was done by polyacrylamide gel electrophoresis. Three bands were obtained at 25.4 KD, 27.5 KD and 29.9 KD. The prepared whole S.equina crude antigen was used in ELLSA test for diagnosis of humans bancroftian filariasis and S. equina infection in donkey as well. The sensitivity and specificity of the test in equines were 100% and 80% respectively, while in human bancroftian filariasis the sensitivity and specificity, was 71% and 71.43% respectively. ELISA proved to be reliable and sensitive test and could be used in sero diagnosis of W. bancrofti and S.equina infection in both humans and equines as well. This test proved to overcome the problem arising from sampling the patients at the midnight, furthermore antigen prepared from S.equina adult worm was more economic than the imported other antigens prepared from D.immitis and others.